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The map label for this gene is pykA [H]

Identifier: 91785090

GI number: 91785090

Start: 4121955

End: 4123391

Strand: Reverse

Name: pykA [H]

Synonym: Bxe_A0690

Alternate gene names: 91785090

Gene position: 4123391-4121955 (Counterclockwise)

Preceding gene: 91785091

Following gene: 91785089

Centisome position: 84.22

GC content: 64.58

Gene sequence:

>1437_bases
ATGCATCGCGCCACCAAGATTGTCGCCACCATCGGACCGGCTTCCAATACGCCGGAAATCCTGCTGCAAATGATTCAGGC
AGGGTTGGACGTGGTGCGGCTCAATTTTTCGCACGGCACCGCCGACGACCACCGCCAGCGCGCCGGATTCGTGCGCGAGG
CGGCCCGCCAGGCCGGCCGCGAAGTCGCCATCATGGCGGACCTGCAAGGCCCGAAGATCCGGGTCGGCAAATTTGAAAAC
GGCAAGATCATGCTGGTCGCCGGCGAGCCGTTCGTGCTCGACGCCGACTGCGAACTCGGCAACGAGCAACGCGCCGGCCT
CGACTACAAAGACCTGCCGCGCGACCTGAAACCGGGCGACGTGCTGTTGCTTAACGACGGCCTGATCGTGCTGAACGTCT
CGCGCGTGATCGGCAACGAGATCCACACGGTCGTCAAGATCGGCGGCGAACTGTCGAACAACAAGGGCATCAACCGCCAG
GGCGGCGGCCTGTCCGCGCCGGCGCTGACCGCCAAGGACATGGAAGATATTCGCACCGCCATGTCGCTCGGCGTCGATTA
TGTCGCGGTATCGTTTCCGAAGAACGCGACCGACATGGAAATGGCCCGCCAGCTGGCGAACATTGCCGGCGCGCCGTTCG
GCGTCAAGCCGAAGATGATCGCCAAGATCGAGCGCGCCGAAGCGATTCCGGCGCTGCAAGGCATTCTCGACGCGTCGGAC
GGCATCATGGTCGCGCGCGGCGATCTGGCCGTGGAAGTGGGGAATGCCGCAGTTCCGGCGCTGCAAAAGCGCATGATTCG
TATGGCGCGCGAATCGAACAAGTTCGTGATCACCGCCACGCAGATGATGGAGTCGATGATCCACGCGCCGGTGCCGACCC
GCGCCGAAGTGTCGGACGTCGCCAACGCCGTGCTGGACGGCACGGACGCGGTGATGCTGTCGGCGGAATCGGCGGCGGGC
AAGTATCCGGTGCAGACCATCGAGACGATGGCGGCGATCTGTATCGAGGCGGAGAAGTCGGAGCAGTCGGAGCTGGACAA
GGATTTCCTCGACCGCACGTTCACGCGGATCGACCAGTCGATCGCAATGGGCGCGCTCTTCACGGCGTTCCATCTCGGCG
CGAAGGCGATCGTGGCGTTGACGGAATCCGGCTCGACCGCGCTGTGGATGTCGCGTCACTGGACTCACGTGCCGATCTTC
GCGCTCACGCCGCGCGTCGGCAGTGAACGGGCAATGGCGCTGTATCGCAACGTGACGTCGCTGCATCTCGATACCAACAC
CGACCGCGACACGGCGTTGCAGCAGGCGTTGGAGACCGTGGTGAGCAAGGGCTACGCGTCGCGCGGCGACATGGTGGTGC
TGACGGTCGGCGAGCCGATGGGGCAGGCCGGCGGCACGAATACGCTGAAGATCGTACGGGTCGGCGAGCCGTATTGA

Upstream 100 bases:

>100_bases
TTCAACCGGCGCGCAGCCCCCGGCAGCATCCTTCGGTGCGCAGCCTCCGCATCCGACTCGCAGCGCTCTTTCCAGCGATC
TCACCCAGACGAGGAGACTC

Downstream 100 bases:

>100_bases
TCGTGCTGCGTGGCAACGGAGGAAGGCCTTCAGCGCCTCCTCCGTTGCCACGCACACGGCTCGATCGGTACCCGGCTGAT
TGACCGAAGCAGGCTGCCAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHRATKIVATIGPASNTPEILLQMIQAGLDVVRLNFSHGTADDHRQRAGFVREAARQAGREVAIMADLQGPKIRVGKFEN
GKIMLVAGEPFVLDADCELGNEQRAGLDYKDLPRDLKPGDVLLLNDGLIVLNVSRVIGNEIHTVVKIGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGVDYVAVSFPKNATDMEMARQLANIAGAPFGVKPKMIAKIERAEAIPALQGILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKFVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVQTIETMAAICIEAEKSEQSELDKDFLDRTFTRIDQSIAMGALFTAFHLGAKAIVALTESGSTALWMSRHWTHVPIF
ALTPRVGSERAMALYRNVTSLHLDTNTDRDTALQQALETVVSKGYASRGDMVVLTVGEPMGQAGGTNTLKIVRVGEPY

Sequences:

>Translated_478_residues
MHRATKIVATIGPASNTPEILLQMIQAGLDVVRLNFSHGTADDHRQRAGFVREAARQAGREVAIMADLQGPKIRVGKFEN
GKIMLVAGEPFVLDADCELGNEQRAGLDYKDLPRDLKPGDVLLLNDGLIVLNVSRVIGNEIHTVVKIGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGVDYVAVSFPKNATDMEMARQLANIAGAPFGVKPKMIAKIERAEAIPALQGILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKFVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVQTIETMAAICIEAEKSEQSELDKDFLDRTFTRIDQSIAMGALFTAFHLGAKAIVALTESGSTALWMSRHWTHVPIF
ALTPRVGSERAMALYRNVTSLHLDTNTDRDTALQQALETVVSKGYASRGDMVVLTVGEPMGQAGGTNTLKIVRVGEPY
>Mature_478_residues
MHRATKIVATIGPASNTPEILLQMIQAGLDVVRLNFSHGTADDHRQRAGFVREAARQAGREVAIMADLQGPKIRVGKFEN
GKIMLVAGEPFVLDADCELGNEQRAGLDYKDLPRDLKPGDVLLLNDGLIVLNVSRVIGNEIHTVVKIGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGVDYVAVSFPKNATDMEMARQLANIAGAPFGVKPKMIAKIERAEAIPALQGILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKFVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVQTIETMAAICIEAEKSEQSELDKDFLDRTFTRIDQSIAMGALFTAFHLGAKAIVALTESGSTALWMSRHWTHVPIF
ALTPRVGSERAMALYRNVTSLHLDTNTDRDTALQQALETVVSKGYASRGDMVVLTVGEPMGQAGGTNTLKIVRVGEPY

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=37.5, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=37.5, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.105476673428, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.105476673428, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=36.105476673428, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.426116838488, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.426116838488, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.8912133891213, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.8912133891213, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=32.1100917431193, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.673640167364, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=39.2781316348195, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.4754098360656, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=37.5770020533881, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=37.5770020533881, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=37.5770020533881, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=37.5770020533881, Blast_Score=254, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=33.8809034907598, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=34.826883910387, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=37.6016260162602, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=37.6016260162602, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=38.3132530120482, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.2883435582822, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24646914, Length=252, Percent_Identity=37.6984126984127, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51267; Mature: 51267

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRATKIVATIGPASNTPEILLQMIQAGLDVVRLNFSHGTADDHRQRAGFVREAARQAGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFENGKIMLVAGEPFVLDADCELGNEQRAGLDYKDLPRDLKPGD
EEEEEEECCCCEEEEEEECCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCC
VLLLNDGLIVLNVSRVIGNEIHTVVKIGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGVDYVAVSFPKNATDMEMARQLANIAGAPFGVKPKMIAKIERAEAIPALQGILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKFVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAESAAGKYPVQTIETMAAICIEAEKSEQSELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IAMGALFTAFHLGAKAIVALTESGSTALWMSRHWTHVPIFALTPRVGSERAMALYRNVTS
HHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCEE
LHLDTNTDRDTALQQALETVVSKGYASRGDMVVLTVGEPMGQAGGTNTLKIVRVGEPY
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MHRATKIVATIGPASNTPEILLQMIQAGLDVVRLNFSHGTADDHRQRAGFVREAARQAGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFENGKIMLVAGEPFVLDADCELGNEQRAGLDYKDLPRDLKPGD
EEEEEEECCCCEEEEEEECCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCC
VLLLNDGLIVLNVSRVIGNEIHTVVKIGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGVDYVAVSFPKNATDMEMARQLANIAGAPFGVKPKMIAKIERAEAIPALQGILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKFVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAESAAGKYPVQTIETMAAICIEAEKSEQSELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IAMGALFTAFHLGAKAIVALTESGSTALWMSRHWTHVPIFALTPRVGSERAMALYRNVTS
HHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCEE
LHLDTNTDRDTALQQALETVVSKGYASRGDMVVLTVGEPMGQAGGTNTLKIVRVGEPY
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]