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The map label for this gene is pgk [H]

Identifier: 91785091

GI number: 91785091

Start: 4123849

End: 4125042

Strand: Reverse

Name: pgk [H]

Synonym: Bxe_A0689

Alternate gene names: 91785091

Gene position: 4125042-4123849 (Counterclockwise)

Preceding gene: 91785092

Following gene: 91785090

Centisome position: 84.26

GC content: 63.82

Gene sequence:

>1194_bases
ATGAACAAGGTATTGCGTCTCTCCGATCTGATCGCCGAAGGCAAGCTATCCGGCAAACGCGTGTTCATCCGCGCCGATCT
GAACGTGCCGCAGGACGATCAAGGCAACATCACCGAAGACACCCGTATCCGCGCTTCCGTGCCGGCCATCAAGGCCGCGC
TCGACGCGGGCGCCGCCGTGATGGTCACGTCGCACCTGGGCCGTCCCACAGAGGGTGATTTCAAGCCTGAAGACTCGCTT
GCGCCGGTCGCGAAACGTCTGGCCGAACTGCTCGGCCGCGAGGTGCCGCTGGTGGCGAACTGGGTCGAAAACGGCGTGAA
CGTCGCGCCGGGCTCGGTCGTGCTGCTGGAAAACTGCCGCGTCAACAAGGGCGAAAAGAAAGATTCCGACGAGCTCGCGC
AGAAAATGGCGAAGCTCTGCGACATCTACGTGAACGACGCATTCGGCACCGCGCACCGCGCCGAAGCCACCACGCACGGT
ATTGCCAGATACGCGCCGGTGGCGTGCGCCGGCCCGCTGCTCGCGGCCGAACTCGAAGCGCTCGGCAAGGCGCTGGGCGC
GCCGAAGCGTCCGCTGGTGGCGATCGTCGCCGGCTCGAAGGTGTCGACCAAGCTGACCATTCTGAAGTCGCTCGCCGACA
AGGTCGATCAACTGATCGTGGGCGGCGGCATTGCCAACACGTTCATGCTGGCCGCGGGCCTGAAGATCGGCAAGTCGCTC
GCCGAAGCCGATCTGGTGAACGAAGCCAAAGCCATCATCGACGCGGCGAAAGCCCGCGGCGCCTCGGTGCCGATCCCGAC
CGACGTGGTCACGGCGAAAGAGTTTTCCCCGACCGCCAAGGCAGAAGTCAAAGCAGTCGCCGACGTTCAGGACGACGACC
TGATCCTCGACATCGGGCCGGAAACGGCCAAAGTGCTCGCCGCGCAACTGGAAAAGGCCGGCACGATCGTGTGGAACGGT
CCGGTCGGCGTGTTCGAATTCGACCAGTTTGGCAACGGCACGAAGACGCTGGCGGAGGCTATCGCCAAATCGTCGGCGTT
CTCGATTGCCGGCGGCGGCGATACGCTTGCTGCGATCGCCAAGTACGGCATCCACGACAAGGTCAGCTATATCTCGACGG
GCGGCGGCGCTTTCCTCGAATTCCTCGAAGGCAAGAAGCTGCCGGCGGTCGAAGTTCTGGAATCGCGGGCTTAA

Upstream 100 bases:

>100_bases
TCAGTGGAACCGCTGCCGTGCCTGAGCGCTGGAGTTCACCGCGCCCGAGTGCCGCGCGCCGTCCGCCACCGCCTTCTCAT
CAACTCGCACACACACGCCC

Downstream 100 bases:

>100_bases
CGTGGCGACGCGCTCCCCAACGGCAAAGTCTGCGAATGCGCGTGGCGGCAAGCCGCGCAACAGCGCAGCGGCATCGGCAG
GGGAGCGCGCGGCGCGCTCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKVLRLSDLIAEGKLSGKRVFIRADLNVPQDDQGNITEDTRIRASVPAIKAALDAGAAVMVTSHLGRPTEGDFKPEDSL
APVAKRLAELLGREVPLVANWVENGVNVAPGSVVLLENCRVNKGEKKDSDELAQKMAKLCDIYVNDAFGTAHRAEATTHG
IARYAPVACAGPLLAAELEALGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
AEADLVNEAKAIIDAAKARGASVPIPTDVVTAKEFSPTAKAEVKAVADVQDDDLILDIGPETAKVLAAQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLESRA

Sequences:

>Translated_397_residues
MNKVLRLSDLIAEGKLSGKRVFIRADLNVPQDDQGNITEDTRIRASVPAIKAALDAGAAVMVTSHLGRPTEGDFKPEDSL
APVAKRLAELLGREVPLVANWVENGVNVAPGSVVLLENCRVNKGEKKDSDELAQKMAKLCDIYVNDAFGTAHRAEATTHG
IARYAPVACAGPLLAAELEALGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
AEADLVNEAKAIIDAAKARGASVPIPTDVVTAKEFSPTAKAEVKAVADVQDDDLILDIGPETAKVLAAQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLESRA
>Mature_397_residues
MNKVLRLSDLIAEGKLSGKRVFIRADLNVPQDDQGNITEDTRIRASVPAIKAALDAGAAVMVTSHLGRPTEGDFKPEDSL
APVAKRLAELLGREVPLVANWVENGVNVAPGSVVLLENCRVNKGEKKDSDELAQKMAKLCDIYVNDAFGTAHRAEATTHG
IARYAPVACAGPLLAAELEALGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
AEADLVNEAKAIIDAAKARGASVPIPTDVVTAKEFSPTAKAEVKAVADVQDDDLILDIGPETAKVLAAQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLESRA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=406, Percent_Identity=42.3645320197044, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=40.8114558472554, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=62.4365482233503, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=46.1916461916462, Blast_Score=309, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=43.8725490196078, Blast_Score=286, Evalue=4e-78,
Organism=Drosophila melanogaster, GI17136394, Length=406, Percent_Identity=41.1330049261084, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24581203, Length=408, Percent_Identity=39.4607843137255, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41275; Mature: 41275

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLRLSDLIAEGKLSGKRVFIRADLNVPQDDQGNITEDTRIRASVPAIKAALDAGAAV
CCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCEE
MVTSHLGRPTEGDFKPEDSLAPVAKRLAELLGREVPLVANWVENGVNVAPGSVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKDSDELAQKMAKLCDIYVNDAFGTAHRAEATTHGIARYAPVACAGPLLAAELEA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEADLVNEAKAIIDAAKARGASVPIPTDVVTAKEFSPTAKAEVKAVADVQDDDLILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHEECCCCCCEEEEECC
ETAKVLAAQLEKAGTIVWNGPVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIA
HHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHH
KYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLESRA
HHCCCCCHHEEECCCHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure
MNKVLRLSDLIAEGKLSGKRVFIRADLNVPQDDQGNITEDTRIRASVPAIKAALDAGAAV
CCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCEE
MVTSHLGRPTEGDFKPEDSLAPVAKRLAELLGREVPLVANWVENGVNVAPGSVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKDSDELAQKMAKLCDIYVNDAFGTAHRAEATTHGIARYAPVACAGPLLAAELEA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEADLVNEAKAIIDAAKARGASVPIPTDVVTAKEFSPTAKAEVKAVADVQDDDLILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHEECCCCCCEEEEECC
ETAKVLAAQLEKAGTIVWNGPVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIA
HHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHH
KYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLESRA
HHCCCCCHHEEECCCHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]