Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 91785031

GI number: 91785031

Start: 4052851

End: 4053321

Strand: Reverse

Name: ogt [H]

Synonym: Bxe_A0749

Alternate gene names: 91785031

Gene position: 4053321-4052851 (Counterclockwise)

Preceding gene: 91785032

Following gene: 91785030

Centisome position: 82.79

GC content: 61.57

Gene sequence:

>471_bases
ATGTTCAACGCAGTGATCGATGCACCGTTCGGCAAGGTTGGCATCCGCCTCGAAGGCGAGGCCGTGCGCGAGATCGTCTA
TCTGCCGGATTCCATGCAAAACGTCGCACCCGGCACGCCGTTGGCGAGGCAGGCGGCCGAGCAGATCGAACGATATTTCG
AGCGTGCTTCGGCGAAGTTCGAGTTGCCGCTTGCCTCGGTGGGCACCGCATTTCAGAAGCGCGTCTGGCAGGCCATCAGC
GACATTCCGCCGGGCGTCGTGCTGACCTATGGGCAATTGGCAAGGCAACTCGGCAGCGCGCCGCGCGCGATCGGTCAGGC
GTGCGGTGCGAATTACTTTCCGATTGTGATTCCATGCCATCGGGTGGTGAGTTCGAGCGGCATCGGCGGTTTTGCGAATC
ACGCCGAAGAGGGTTTCTTCCGCAACGTGAAGCGCTGGCTGCTGGCGCATGAAGGCATTCCCTACGCATGA

Upstream 100 bases:

>100_bases
CGGCAATCTATGGGGCACTGGCGTGCGCCGCGCATCTGCGCGATATGCAGGTGCGCCAGCTTGTGAGGCGGAACGTCGCG
CCAGTGAGGAGCAGGCAATC

Downstream 100 bases:

>100_bases
GCGATACCTTGATCGACGACGCGCCCGCCGCGTCACCTCTCTTTGTTGCCAGCTCGGCGTCGATCGACGCATTCTGCGAC
GCGCTGTGGCTCGAACATGG

Product: methylated-DNA-(protein)-cysteine S- methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MFNAVIDAPFGKVGIRLEGEAVREIVYLPDSMQNVAPGTPLARQAAEQIERYFERASAKFELPLASVGTAFQKRVWQAIS
DIPPGVVLTYGQLARQLGSAPRAIGQACGANYFPIVIPCHRVVSSSGIGGFANHAEEGFFRNVKRWLLAHEGIPYA

Sequences:

>Translated_156_residues
MFNAVIDAPFGKVGIRLEGEAVREIVYLPDSMQNVAPGTPLARQAAEQIERYFERASAKFELPLASVGTAFQKRVWQAIS
DIPPGVVLTYGQLARQLGSAPRAIGQACGANYFPIVIPCHRVVSSSGIGGFANHAEEGFFRNVKRWLLAHEGIPYA
>Mature_156_residues
MFNAVIDAPFGKVGIRLEGEAVREIVYLPDSMQNVAPGTPLARQAAEQIERYFERASAKFELPLASVGTAFQKRVWQAIS
DIPPGVVLTYGQLARQLGSAPRAIGQACGANYFPIVIPCHRVVSSSGIGGFANHAEEGFFRNVKRWLLAHEGIPYA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=173, Percent_Identity=32.3699421965318, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1787596, Length=133, Percent_Identity=38.3458646616541, Blast_Score=83, Evalue=9e-18,
Organism=Escherichia coli, GI1788542, Length=95, Percent_Identity=41.0526315789474, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=93, Percent_Identity=35.4838709677419, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16956; Mature: 16956

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNAVIDAPFGKVGIRLEGEAVREIVYLPDSMQNVAPGTPLARQAAEQIERYFERASAKF
CCCCCCCCCCCCEEEEECHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
ELPLASVGTAFQKRVWQAISDIPPGVVLTYGQLARQLGSAPRAIGQACGANYFPIVIPCH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVSSSGIGGFANHAEEGFFRNVKRWLLAHEGIPYA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFNAVIDAPFGKVGIRLEGEAVREIVYLPDSMQNVAPGTPLARQAAEQIERYFERASAKF
CCCCCCCCCCCCEEEEECHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
ELPLASVGTAFQKRVWQAISDIPPGVVLTYGQLARQLGSAPRAIGQACGANYFPIVIPCH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVSSSGIGGFANHAEEGFFRNVKRWLLAHEGIPYA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]