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The map label for this gene is potI [H]

Identifier: 91783858

GI number: 91783858

Start: 2775142

End: 2775960

Strand: Reverse

Name: potI [H]

Synonym: Bxe_A1953

Alternate gene names: 91783858

Gene position: 2775960-2775142 (Counterclockwise)

Preceding gene: 91783859

Following gene: 91783854

Centisome position: 56.7

GC content: 58.12

Gene sequence:

>819_bases
ATGAAGCCGAACCGGATTTTGCAGTTCATTGCCCTCGGAATCGGGTTTGTGTTTCTGTACGTGCCGATCGTGAGTCTGAT
TGTGTATTCGTTCAACGAATCGCAACTGGTCACGGTGTGGACGCGTTTTTCGACACGCTGGTATGCGGCGTTGCTACAGG
ATGACGAACTGATCGCGGCGGCGTGGTTGTCGTTGCGAGTGGCGTTGTTGACGGCGTTTGCGTCGGTGATCATCGGCACG
TGGGCCGGGTTCGTGCTCGCGCGCATGGGACGGTTTCGCGGCTTCACGTTGTATACGGGGATGATCAACGCGCCGTTGGT
GATTCCCGAGGTGATTCAGGGCATTTCGCTGCTGCTCCTGTTCATCGAAATGGCCAAGTGGTTGGGATGGCCGGCCGGAC
GCGGCATTTTCACGATCTGGATCGGGCACGTGATGCTGTGCATCTCTTATGTGGCGATCATTGTGCAGTCGAGGGTGAAG
GAACTGCATCCTTCGCTCGAAGAGGCGGCGCTGGATCTTGGGGCTACGCCGTTGCGGGTGTTTTTCTTCATCACGTTGCC
GCTGATTTCGCAGGCGCTTGTGTCGGGCTGGTTGTTGTCGTTTACGTTGTCGATCGACGATCTGGTGCTGTCGGCGTTTT
TGTCCGGGCCCGGTTCGACCACGCTGCCGCTGGTCGTTTTCTCGCGCGTTCGTCTGGGGCTGAATCCGGAGATGAACGCT
TTGGCCACGCTGTTTATCGCGGTGGTCACGGTTGGGGTCGTGGTCGCCAACTACTTCATGCAGCGGGCGGAGAGAAAACG
GGCCGTGATGGCGGTTTAG

Upstream 100 bases:

>100_bases
CGATGGCATCCGCCGTGACGTGCGCGATGGTGTTGCTGCTATTGGTGCCGATGGCGCTTTTCCAGTACTCGCAGGCAAAG
GCGATGGAGGAAAAGCGCTG

Downstream 100 bases:

>100_bases
GCAATGGGTGGAAGCGGTTGGGCGTGTTGCACGGACCCAGCCGGCCACCCTGAAAAACCTCAAACCTTATACCCAATCCG
AACGCCGCCCCAATGCCGCC

Product: ABC polyamine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKPNRILQFIALGIGFVFLYVPIVSLIVYSFNESQLVTVWTRFSTRWYAALLQDDELIAAAWLSLRVALLTAFASVIIGT
WAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFIEMAKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVK
ELHPSLEEAALDLGATPLRVFFFITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIAVVTVGVVVANYFMQRAERKRAVMAV

Sequences:

>Translated_272_residues
MKPNRILQFIALGIGFVFLYVPIVSLIVYSFNESQLVTVWTRFSTRWYAALLQDDELIAAAWLSLRVALLTAFASVIIGT
WAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFIEMAKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVK
ELHPSLEEAALDLGATPLRVFFFITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIAVVTVGVVVANYFMQRAERKRAVMAV
>Mature_272_residues
MKPNRILQFIALGIGFVFLYVPIVSLIVYSFNESQLVTVWTRFSTRWYAALLQDDELIAAAWLSLRVALLTAFASVIIGT
WAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFIEMAKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVK
ELHPSLEEAALDLGATPLRVFFFITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIAVVTVGVVVANYFMQRAERKRAVMAV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=260, Percent_Identity=56.9230769230769, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=37.6569037656904, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788764, Length=228, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30100; Mature: 30100

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNRILQFIALGIGFVFLYVPIVSLIVYSFNESQLVTVWTRFSTRWYAALLQDDELIAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCHHHHH
AWLSLRVALLTAFASVIIGTWAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHHH
FIEMAKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVKELHPSLEEAALDLGATPLRV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
FFFITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIAVVTVGVVVANYFMQRAERKRAVMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPNRILQFIALGIGFVFLYVPIVSLIVYSFNESQLVTVWTRFSTRWYAALLQDDELIAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCHHHHH
AWLSLRVALLTAFASVIIGTWAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHHH
FIEMAKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVKELHPSLEEAALDLGATPLRV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
FFFITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIAVVTVGVVVANYFMQRAERKRAVMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]