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The map label for this gene is potH [H]

Identifier: 91783859

GI number: 91783859

Start: 2775960

End: 2776889

Strand: Reverse

Name: potH [H]

Synonym: Bxe_A1952

Alternate gene names: 91783859

Gene position: 2776889-2775960 (Counterclockwise)

Preceding gene: 91783860

Following gene: 91783858

Centisome position: 56.72

GC content: 58.17

Gene sequence:

>930_bases
ATGAAGCGCTCGTTCAATTCCTTCGCCAACTGGCCGGTGCGGCGCTTCAACCTGACCGGCCGCACGGCGGTGGTGGCCGG
TCCGTTCATCTGGCTGCTGCTGTTTTTTCTCGTGCCGTTCCTGCTGGTCGTCAAGATCAGCTTCGCCGACTTGCAGCTCG
GCATTCCGCCGTACACGGAGTTGACCAACTTCGCGGACGGCGCGATTCACATCACGCTCGACCTGTCGCATTACGCGTTT
CTGCTGACCGACAGCCTCTATTTCGCGACCTACGTGAATTCCGTGGTGGTGGCCGCGATCTCGACGCTGTTGTGTTTGCT
GATCGGCTACCCGATGGCGTATTACATCGCCCGTTCGAATCCGGCCAGCCGCAATCTGCTGATGATGGCCGTGATGCTGC
CGTTCTGGACGTCGTTTCTGATTCGCGTGTATGCGTGGATCGGCATCCTGAAGAACAATGGCTTGCTGAATAACTTCCTG
ATGTCGATCGGGCTGATTCACTCGCCGATCGAGCTGTATCACACGAATACCGCCGTTTATATCGGCATGGTCTATTCGTA
TCTGCCTTTTCTCGTCATGCCGCTGTACGCGCATCTGGTGAAAATGGACATGACGCTGCTGGAGGCCGCCTACGACCTCG
GAGCGCGGCCCTGGAAGGCATTCTGGCAGATCACGTTGCCGCTCTCGAAGAACGGCATCATCGCCGGCTGTTTGCTGGTG
TTCATTCCGGCAGTGGGCGAGTACGTGATTCCCGAATTGCTGGGCGGCGCGAACACGCTGATGATCGGCCGCGTGATGTG
GAACGAGTTCTTCGACAATGCCGATTGGCCGATGGCATCCGCCGTGACGTGCGCGATGGTGTTGCTGCTATTGGTGCCGA
TGGCGCTTTTCCAGTACTCGCAGGCAAAGGCGATGGAGGAAAAGCGCTGA

Upstream 100 bases:

>100_bases
CGAATGTGACGAGCCTCGCGTTGACGGAGATCGATCCGCCCACCTGGGGCGACGAAGTGTACGTGCGCTGGAGCGCGTCG
GCCGGCGTGGTGCTGACGTC

Downstream 100 bases:

>100_bases
TGAAGCCGAACCGGATTTTGCAGTTCATTGCCCTCGGAATCGGGTTTGTGTTTCTGTACGTGCCGATCGTGAGTCTGATT
GTGTATTCGTTCAACGAATC

Product: ABC polyamine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKRSFNSFANWPVRRFNLTGRTAVVAGPFIWLLLFFLVPFLLVVKISFADLQLGIPPYTELTNFADGAIHITLDLSHYAF
LLTDSLYFATYVNSVVVAAISTLLCLLIGYPMAYYIARSNPASRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFL
MSIGLIHSPIELYHTNTAVYIGMVYSYLPFLVMPLYAHLVKMDMTLLEAAYDLGARPWKAFWQITLPLSKNGIIAGCLLV
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYSQAKAMEEKR

Sequences:

>Translated_309_residues
MKRSFNSFANWPVRRFNLTGRTAVVAGPFIWLLLFFLVPFLLVVKISFADLQLGIPPYTELTNFADGAIHITLDLSHYAF
LLTDSLYFATYVNSVVVAAISTLLCLLIGYPMAYYIARSNPASRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFL
MSIGLIHSPIELYHTNTAVYIGMVYSYLPFLVMPLYAHLVKMDMTLLEAAYDLGARPWKAFWQITLPLSKNGIIAGCLLV
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYSQAKAMEEKR
>Mature_309_residues
MKRSFNSFANWPVRRFNLTGRTAVVAGPFIWLLLFFLVPFLLVVKISFADLQLGIPPYTELTNFADGAIHITLDLSHYAF
LLTDSLYFATYVNSVVVAAISTLLCLLIGYPMAYYIARSNPASRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFL
MSIGLIHSPIELYHTNTAVYIGMVYSYLPFLVMPLYAHLVKMDMTLLEAAYDLGARPWKAFWQITLPLSKNGIIAGCLLV
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYSQAKAMEEKR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=288, Percent_Identity=59.375, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=39.426523297491, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=27.5193798449612, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34801; Mature: 34801

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSFNSFANWPVRRFNLTGRTAVVAGPFIWLLLFFLVPFLLVVKISFADLQLGIPPYTE
CCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHH
LTNFADGAIHITLDLSHYAFLLTDSLYFATYVNSVVVAAISTLLCLLIGYPMAYYIARSN
HHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
PASRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMSIGLIHSPIELYHTNTAVY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHEECCHHHH
IGMVYSYLPFLVMPLYAHLVKMDMTLLEAAYDLGARPWKAFWQITLPLSKNGIIAGCLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHH
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QAKAMEEKR
HHHHHHCCC
>Mature Secondary Structure
MKRSFNSFANWPVRRFNLTGRTAVVAGPFIWLLLFFLVPFLLVVKISFADLQLGIPPYTE
CCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHH
LTNFADGAIHITLDLSHYAFLLTDSLYFATYVNSVVVAAISTLLCLLIGYPMAYYIARSN
HHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
PASRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMSIGLIHSPIELYHTNTAVY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHEECCHHHH
IGMVYSYLPFLVMPLYAHLVKMDMTLLEAAYDLGARPWKAFWQITLPLSKNGIIAGCLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHH
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QAKAMEEKR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]