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The map label for this gene is htrB [H]

Identifier: 91783849

GI number: 91783849

Start: 2762594

End: 2763472

Strand: Reverse

Name: htrB [H]

Synonym: Bxe_A1962

Alternate gene names: 91783849

Gene position: 2763472-2762594 (Counterclockwise)

Preceding gene: 91783850

Following gene: 91783848

Centisome position: 56.45

GC content: 62.57

Gene sequence:

>879_bases
GTGAAAAGTCGAATCCTGATCTGTCTGATGACAGGTCTGCTGCGCGCGTTTGCCTTTCTGCCATACAGCGTTGTCGCGCG
GATCGGCACCGGTATCGGTGCCTTGCTGTACCGGATTCCGAGCACGCGCCGGCGCGTCGTGCATACCAACCTGAAGCTGT
GTTTTCCGGACATGAGCGACGAGGCGCGCGAACGTCTGGCGCGCGCGCACTTCTGTCACGTGATCCGCAGTTATGTCGAG
CGCGGCCCGCAGTGGTTCGGCAATGCGCAGGCGCTGGCTCGCCTCGTCGAGGTGGAGAGCGCGATCGATCTCTCGGATAT
GGCGGGAAAGCCGACCATTTTCGTCGGCTTCCATTTCGTTGGCATCGAAGCGGGCTGCATGATGTATTCGACGCGGCATC
CGGTCGCCTCGCTCTATACGCCCATGTCGAACCTGCTGCTCGACGCGATGGCCAAGCGTCAGCGCGGACGCTTCGGCACG
GAGATGATTCCGCGCAGCGATAGCGTGCGGCGGGCGTTGCGCACGCTACGCGAAGGCAAGCCGGTCATGCTGGCCGCCGA
CATGGATTTCGGCTTGCGGGATTCGGTGTTCGTGCCGTTCTTCGGCGTGCCGGCCTGCACGCTGACCTCCGTCTCGCGGA
TGGCGCGTGCGGCGAATGCGCGGGTGGTGCCGTTCGTGACGGAAGTGCTGCCGGATTATCGCGGCTACAAGCTGAATATC
TTTGAGGCGCTGGGCGATTTTCCGTCCGACGACGTGGCGGTCGACGCGCGGCGTATCACGGCGTTTCTGGAGACGCAGGT
TAGACGGATTCCTGATCAGTATTATTGGGTGCACAGGCGCTTCAAGAATCGGCCGGCGGGCGAGGCGAGTGTTTATTGA

Upstream 100 bases:

>100_bases
GACCCGGTATGACGCGCGCGCAAGTCACTTCGTCCGCTGGCAATTTCGAGGTTGCAGCGGCCGGCGTGTTTGCCATCCAG
CACCCACCGGGTCCCGCCTT

Downstream 100 bases:

>100_bases
TGCTGCCGGTGTCGGACTGCGGCAATGAGCGGGGCGCTGCGCTGGTCCTGGTTTGAGCAGAGGGCGTCGTTAAACGCGTC
GGTGGAAGAATCTGGTGCGC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKSRILICLMTGLLRAFAFLPYSVVARIGTGIGALLYRIPSTRRRVVHTNLKLCFPDMSDEARERLARAHFCHVIRSYVE
RGPQWFGNAQALARLVEVESAIDLSDMAGKPTIFVGFHFVGIEAGCMMYSTRHPVASLYTPMSNLLLDAMAKRQRGRFGT
EMIPRSDSVRRALRTLREGKPVMLAADMDFGLRDSVFVPFFGVPACTLTSVSRMARAANARVVPFVTEVLPDYRGYKLNI
FEALGDFPSDDVAVDARRITAFLETQVRRIPDQYYWVHRRFKNRPAGEASVY

Sequences:

>Translated_292_residues
MKSRILICLMTGLLRAFAFLPYSVVARIGTGIGALLYRIPSTRRRVVHTNLKLCFPDMSDEARERLARAHFCHVIRSYVE
RGPQWFGNAQALARLVEVESAIDLSDMAGKPTIFVGFHFVGIEAGCMMYSTRHPVASLYTPMSNLLLDAMAKRQRGRFGT
EMIPRSDSVRRALRTLREGKPVMLAADMDFGLRDSVFVPFFGVPACTLTSVSRMARAANARVVPFVTEVLPDYRGYKLNI
FEALGDFPSDDVAVDARRITAFLETQVRRIPDQYYWVHRRFKNRPAGEASVY
>Mature_292_residues
MKSRILICLMTGLLRAFAFLPYSVVARIGTGIGALLYRIPSTRRRVVHTNLKLCFPDMSDEARERLARAHFCHVIRSYVE
RGPQWFGNAQALARLVEVESAIDLSDMAGKPTIFVGFHFVGIEAGCMMYSTRHPVASLYTPMSNLLLDAMAKRQRGRFGT
EMIPRSDSVRRALRTLREGKPVMLAADMDFGLRDSVFVPFFGVPACTLTSVSRMARAANARVVPFVTEVLPDYRGYKLNI
FEALGDFPSDDVAVDARRITAFLETQVRRIPDQYYWVHRRFKNRPAGEASVY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=290, Percent_Identity=35.5172413793103, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87082094, Length=284, Percent_Identity=32.3943661971831, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788161, Length=276, Percent_Identity=25.7246376811594, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 32993; Mature: 32993

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRILICLMTGLLRAFAFLPYSVVARIGTGIGALLYRIPSTRRRVVHTNLKLCFPDMSD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHCCCEEECCCCCH
EARERLARAHFCHVIRSYVERGPQWFGNAQALARLVEVESAIDLSDMAGKPTIFVGFHFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCEEEEEEEEE
GIEAGCMMYSTRHPVASLYTPMSNLLLDAMAKRQRGRFGTEMIPRSDSVRRALRTLREGK
CHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
PVMLAADMDFGLRDSVFVPFFGVPACTLTSVSRMARAANARVVPFVTEVLPDYRGYKLNI
CEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEH
FEALGDFPSDDVAVDARRITAFLETQVRRIPDQYYWVHRRFKNRPAGEASVY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKSRILICLMTGLLRAFAFLPYSVVARIGTGIGALLYRIPSTRRRVVHTNLKLCFPDMSD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHCCCEEECCCCCH
EARERLARAHFCHVIRSYVERGPQWFGNAQALARLVEVESAIDLSDMAGKPTIFVGFHFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCEEEEEEEEE
GIEAGCMMYSTRHPVASLYTPMSNLLLDAMAKRQRGRFGTEMIPRSDSVRRALRTLREGK
CHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
PVMLAADMDFGLRDSVFVPFFGVPACTLTSVSRMARAANARVVPFVTEVLPDYRGYKLNI
CEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEH
FEALGDFPSDDVAVDARRITAFLETQVRRIPDQYYWVHRRFKNRPAGEASVY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]