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The map label for this gene is vanB [H]

Identifier: 91783703

GI number: 91783703

Start: 2597886

End: 2598863

Strand: Direct

Name: vanB [H]

Synonym: Bxe_A2111

Alternate gene names: 91783703

Gene position: 2597886-2598863 (Clockwise)

Preceding gene: 91783702

Following gene: 91783707

Centisome position: 53.06

GC content: 63.29

Gene sequence:

>978_bases
ATGGAAAGAACCACCTTTAACGTGCGCGTAGACAGCGTCCGTGACGAAGCGCACGGCGTGCGCTCGTTCAGCGTGTCGCG
TCTGGACGGCCAGCCGTTCGACCACTACGAACCCGGCGCACATATCGACGTTACCGGACCTTCCGGCGTCACGCGGCAAT
ACTCGTTGTGCGGCGATCCGGATCACCGTGAAACGCATCTGTTTGCAGTGAAGCGTGAAGACGCCTCGCGCGGCGGTTCG
CGTTCGCTGCATGACGAAGTCACGGTTGGCAGCCAATTGACCATCGGCGCACCGCGCAATCTGTTCCAACTCGCGCCGGG
CGCCGAAGAACATGTTCTGATCGCCGCCGGTATCGGCGTAACGCCACTCCTGAGCATGGCGTACCGCTTGCTCAAACAGC
GGCAACCCTTCGTTCTGCACTATTTCGCGCGCAGTGCCGAACACGCGGCGTTCTTGCCGCTGCTCTCACGCGCGCCGTTC
AGTGAATACGTGAAGCTGCATTTCGGCGTCGAGCGCGATCGGCTCGATGCGGTACTCGGCGATTGTCTCGCGGATGCGCG
CGACGGCACGCACATTTACACGTGCGGGCCGTCGGCTTTCATGGAACGCGTGGTGGCGATCGGCGAAACGCGTGTCCAGC
CGCAAGCGATTCACCTCGAACGATTCGCGGCCGAGCCGGCTTCGACCACGAGCGCCGAATCCGCCACGCTCGATACCTTC
GACGTGCGAATTGCAAGCAGCGGCGCGACCGTGCGTGTGGACAGGAACACGACTATCGTCGCGGCGCTCGCGTCGATCGG
CATCGAAGTGGATACGTCGTGCGGCGAAGGTGTGTGCGGCACGTGCATGGTCGACGTGGTGAGCGGCACGCCCGAGCATC
GCGACCATTGCCTGAGCAAAGCGGAACGCGCGAGCGGCAAGGTGATCTGCTGCTGCATCTCGCGCGCGGCATCGCCGGTT
CTGGTGCTGGATCTGTAA

Upstream 100 bases:

>100_bases
ATTTGCCTGATTTGGCGTGGTTTCTTATCCTTGATGCACTTGCTGCACAACCGCATGCGATCCGACGCATGCGCCGTCCG
CTCATTCTGCCGGCCCTATC

Downstream 100 bases:

>100_bases
AGACCGGCCAGGGTGCTGCAATCGCAGCACCTTTCCCAAACGCCGGCTGTTACCGGTCGTCGTCGCGAATTGACGACGCA
TGCCGGCACAGCGCGCGCAC

Product: ferredoxin-oxidoreductase FAD/NAD(P)- binding, ring hydroxylating dioxygenase reductase subunit

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MERTTFNVRVDSVRDEAHGVRSFSVSRLDGQPFDHYEPGAHIDVTGPSGVTRQYSLCGDPDHRETHLFAVKREDASRGGS
RSLHDEVTVGSQLTIGAPRNLFQLAPGAEEHVLIAAGIGVTPLLSMAYRLLKQRQPFVLHYFARSAEHAAFLPLLSRAPF
SEYVKLHFGVERDRLDAVLGDCLADARDGTHIYTCGPSAFMERVVAIGETRVQPQAIHLERFAAEPASTTSAESATLDTF
DVRIASSGATVRVDRNTTIVAALASIGIEVDTSCGEGVCGTCMVDVVSGTPEHRDHCLSKAERASGKVICCCISRAASPV
LVLDL

Sequences:

>Translated_325_residues
MERTTFNVRVDSVRDEAHGVRSFSVSRLDGQPFDHYEPGAHIDVTGPSGVTRQYSLCGDPDHRETHLFAVKREDASRGGS
RSLHDEVTVGSQLTIGAPRNLFQLAPGAEEHVLIAAGIGVTPLLSMAYRLLKQRQPFVLHYFARSAEHAAFLPLLSRAPF
SEYVKLHFGVERDRLDAVLGDCLADARDGTHIYTCGPSAFMERVVAIGETRVQPQAIHLERFAAEPASTTSAESATLDTF
DVRIASSGATVRVDRNTTIVAALASIGIEVDTSCGEGVCGTCMVDVVSGTPEHRDHCLSKAERASGKVICCCISRAASPV
LVLDL
>Mature_325_residues
MERTTFNVRVDSVRDEAHGVRSFSVSRLDGQPFDHYEPGAHIDVTGPSGVTRQYSLCGDPDHRETHLFAVKREDASRGGS
RSLHDEVTVGSQLTIGAPRNLFQLAPGAEEHVLIAAGIGVTPLLSMAYRLLKQRQPFVLHYFARSAEHAAFLPLLSRAPF
SEYVKLHFGVERDRLDAVLGDCLADARDGTHIYTCGPSAFMERVVAIGETRVQPQAIHLERFAAEPASTTSAESATLDTF
DVRIASSGATVRVDRNTTIVAALASIGIEVDTSCGEGVCGTCMVDVVSGTPEHRDHCLSKAERASGKVICCCISRAASPV
LVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=325, Percent_Identity=37.8461538461538, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787098, Length=314, Percent_Identity=28.6624203821656, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787658, Length=325, Percent_Identity=25.8461538461538, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35056; Mature: 35056

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTTFNVRVDSVRDEAHGVRSFSVSRLDGQPFDHYEPGAHIDVTGPSGVTRQYSLCGDP
CCCEEEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCEEEECCCCC
DHRETHLFAVKREDASRGGSRSLHDEVTVGSQLTIGAPRNLFQLAPGAEEHVLIAAGIGV
CCCCEEEEEEECCCCCCCCCCCCCHHEECCCEEEECCCCCCEECCCCCCCCEEEEECCCH
TPLLSMAYRLLKQRQPFVLHYFARSAEHAAFLPLLSRAPFSEYVKLHFGVERDRLDAVLG
HHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHH
DCLADARDGTHIYTCGPSAFMERVVAIGETRVQPQAIHLERFAAEPASTTSAESATLDTF
HHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEE
DVRIASSGATVRVDRNTTIVAALASIGIEVDTSCGEGVCGTCMVDVVSGTPEHRDHCLSK
EEEEECCCCEEEEECCCEEEEEHHHCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
AERASGKVICCCISRAASPVLVLDL
HHHCCCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MERTTFNVRVDSVRDEAHGVRSFSVSRLDGQPFDHYEPGAHIDVTGPSGVTRQYSLCGDP
CCCEEEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCEEEECCCCC
DHRETHLFAVKREDASRGGSRSLHDEVTVGSQLTIGAPRNLFQLAPGAEEHVLIAAGIGV
CCCCEEEEEEECCCCCCCCCCCCCHHEECCCEEEECCCCCCEECCCCCCCCEEEEECCCH
TPLLSMAYRLLKQRQPFVLHYFARSAEHAAFLPLLSRAPFSEYVKLHFGVERDRLDAVLG
HHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHH
DCLADARDGTHIYTCGPSAFMERVVAIGETRVQPQAIHLERFAAEPASTTSAESATLDTF
HHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEE
DVRIASSGATVRVDRNTTIVAALASIGIEVDTSCGEGVCGTCMVDVVSGTPEHRDHCLSK
EEEEECCCCEEEEECCCEEEEEHHHCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
AERASGKVICCCISRAASPVLVLDL
HHHCCCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579070 [H]