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The map label for this gene is aes [H]

Identifier: 91783548

GI number: 91783548

Start: 2409950

End: 2410915

Strand: Direct

Name: aes [H]

Synonym: Bxe_A2267

Alternate gene names: 91783548

Gene position: 2409950-2410915 (Clockwise)

Preceding gene: 91783547

Following gene: 91783551

Centisome position: 49.22

GC content: 66.15

Gene sequence:

>966_bases
ATGCCGCTGAATCCCAAGATCGAGCAGGTGCTCGACATGATCGAGCGGGCCAGGCGCCCGAAGTTCCATGAAATGACCGC
GCAAGACGCGCGGGCCTCGTACGAAAAAAGCGCGCCGATCCTGGAGATCGCCAGCGCGCCGATGTTCGCGATCGAAGATC
TGCGGATCCCGACACGCGACGGCGCGACGATTCGCGCGCGTCTTTACCATCCGGTCGAGCCGGGCTGGGCGGAGCCCGCG
CCGGCGCTGGTGTACTACCACGGCGGCGGCTTCACGGTGGGCAGCGTGAATACGCACGATGCGCTGTGCCGGATGTTCGC
GCGCGACGGCAAGTGCACGGTGTTGTCGGTGGATTACCGACTCGCACCGGAATACAAGTTTCCCACTGCCGTCGAAGACG
CTTTCGACGCATTGACCTGGCTGCACGCGCACGCCGCCGAATACGGCGTGGACGCGAGCCGGATCGCGGTGGGCGGCGAT
AGCGCGGGCGGTACGTTGGCCACGGTCTGCGCGGTGCTCGCACGCGAGCACGGCATCGAACCCGTGCTGCAATTGCTGAT
CTACCCGGGCACGACCGGCCATCAGCAAACCGATTCGCACTCGCGCTTCGCCAACGGGTTTCTGTTGTCGGGCGACACGA
TTCAATGGTTCTTCGAGCACTACGTGCGCGACAGAAGCGACCGTGACGACTGGCGTTTCGCGCCGCTCGACGGCCGGCAC
GGCGCGCCCGATTTCCGCGGCGTTGCGCCCGCATGGATTGCCACAGCCGAATACGATCCCCTGAGCGACGAGGGCGACGC
CTACGCGGAGAAATTGCGCGCGCTGGGCAACCGGGTCACGCTCAAACGCTATCCGGGCATGATCCACGAGTTCTTCAAGA
TGGGTGGCTTCGTGCCGGAGGTCGGCGAGGCGCATGCGGACGCCGCGGCGGCATTGCGCGCGGCGTTCGGGGTCGATGCG
CACTAG

Upstream 100 bases:

>100_bases
AGGGCGCCGGCTAGGCGAAGCGCTCATCGCAACGCCGGCGCCGCCGACACCGCGGCGCTGTTTTTCAAACCTTCGGCGCT
CACGCGCTATCAGACGGACC

Downstream 100 bases:

>100_bases
GCGCGCCTCGCCGGGCGGCGGCCAATGGCCAGCGAGCCGCCGCGCAACCGTCACACCCTCTCGCGTTCGATATTGAAAGC
GAAGCTGCGCTCGAAATCGC

Product: putative esterase/lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MPLNPKIEQVLDMIERARRPKFHEMTAQDARASYEKSAPILEIASAPMFAIEDLRIPTRDGATIRARLYHPVEPGWAEPA
PALVYYHGGGFTVGSVNTHDALCRMFARDGKCTVLSVDYRLAPEYKFPTAVEDAFDALTWLHAHAAEYGVDASRIAVGGD
SAGGTLATVCAVLAREHGIEPVLQLLIYPGTTGHQQTDSHSRFANGFLLSGDTIQWFFEHYVRDRSDRDDWRFAPLDGRH
GAPDFRGVAPAWIATAEYDPLSDEGDAYAEKLRALGNRVTLKRYPGMIHEFFKMGGFVPEVGEAHADAAAALRAAFGVDA
H

Sequences:

>Translated_321_residues
MPLNPKIEQVLDMIERARRPKFHEMTAQDARASYEKSAPILEIASAPMFAIEDLRIPTRDGATIRARLYHPVEPGWAEPA
PALVYYHGGGFTVGSVNTHDALCRMFARDGKCTVLSVDYRLAPEYKFPTAVEDAFDALTWLHAHAAEYGVDASRIAVGGD
SAGGTLATVCAVLAREHGIEPVLQLLIYPGTTGHQQTDSHSRFANGFLLSGDTIQWFFEHYVRDRSDRDDWRFAPLDGRH
GAPDFRGVAPAWIATAEYDPLSDEGDAYAEKLRALGNRVTLKRYPGMIHEFFKMGGFVPEVGEAHADAAAALRAAFGVDA
H
>Mature_320_residues
PLNPKIEQVLDMIERARRPKFHEMTAQDARASYEKSAPILEIASAPMFAIEDLRIPTRDGATIRARLYHPVEPGWAEPAP
ALVYYHGGGFTVGSVNTHDALCRMFARDGKCTVLSVDYRLAPEYKFPTAVEDAFDALTWLHAHAAEYGVDASRIAVGGDS
AGGTLATVCAVLAREHGIEPVLQLLIYPGTTGHQQTDSHSRFANGFLLSGDTIQWFFEHYVRDRSDRDDWRFAPLDGRHG
APDFRGVAPAWIATAEYDPLSDEGDAYAEKLRALGNRVTLKRYPGMIHEFFKMGGFVPEVGEAHADAAAALRAAFGVDAH

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=293, Percent_Identity=29.6928327645051, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI206597554, Length=291, Percent_Identity=28.8659793814433, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157041239, Length=181, Percent_Identity=32.5966850828729, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI21328446, Length=110, Percent_Identity=37.2727272727273, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI61966717, Length=217, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786682, Length=278, Percent_Identity=33.8129496402878, Blast_Score=137, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71996133, Length=306, Percent_Identity=27.4509803921569, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=290, Percent_Identity=26.551724137931, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=122, Percent_Identity=40.1639344262295, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24656084, Length=109, Percent_Identity=38.5321100917431, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656076, Length=109, Percent_Identity=38.5321100917431, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20130169, Length=109, Percent_Identity=38.5321100917431, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 35310; Mature: 35179

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNPKIEQVLDMIERARRPKFHEMTAQDARASYEKSAPILEIASAPMFAIEDLRIPTRD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHCCCCCCCC
GATIRARLYHPVEPGWAEPAPALVYYHGGGFTVGSVNTHDALCRMFARDGKCTVLSVDYR
CCEEEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEECEE
LAPEYKFPTAVEDAFDALTWLHAHAAEYGVDASRIAVGGDSAGGTLATVCAVLAREHGIE
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHHHHHHHHCCHH
PVLQLLIYPGTTGHQQTDSHSRFANGFLLSGDTIQWFFEHYVRDRSDRDDWRFAPLDGRH
HHHHHHHCCCCCCCCCCCCHHHHHCCEEECCCHHHHHHHHHHHCCCCCCCCEECCCCCCC
GAPDFRGVAPAWIATAEYDPLSDEGDAYAEKLRALGNRVTLKRYPGMIHEFFKMGGFVPE
CCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHCCCEEHHHCCCHHHHHHHHCCCCCC
VGEAHADAAAALRAAFGVDAH
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLNPKIEQVLDMIERARRPKFHEMTAQDARASYEKSAPILEIASAPMFAIEDLRIPTRD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHCCCCCCCC
GATIRARLYHPVEPGWAEPAPALVYYHGGGFTVGSVNTHDALCRMFARDGKCTVLSVDYR
CCEEEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEECEE
LAPEYKFPTAVEDAFDALTWLHAHAAEYGVDASRIAVGGDSAGGTLATVCAVLAREHGIE
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHHHHHHHHCCHH
PVLQLLIYPGTTGHQQTDSHSRFANGFLLSGDTIQWFFEHYVRDRSDRDDWRFAPLDGRH
HHHHHHHCCCCCCCCCCCCHHHHHCCEEECCCHHHHHHHHHHHCCCCCCCCEECCCCCCC
GAPDFRGVAPAWIATAEYDPLSDEGDAYAEKLRALGNRVTLKRYPGMIHEFFKMGGFVPE
CCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHCCCEEHHHCCCHHHHHHHHCCCCCC
VGEAHADAAAALRAAFGVDAH
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA