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The map label for this gene is cysW [H]

Identifier: 91783351

GI number: 91783351

Start: 2195109

End: 2196149

Strand: Direct

Name: cysW [H]

Synonym: Bxe_A2468

Alternate gene names: 91783351

Gene position: 2195109-2196149 (Clockwise)

Preceding gene: 91783350

Following gene: 91783352

Centisome position: 44.84

GC content: 64.27

Gene sequence:

>1041_bases
GTGAATGGCGTGCGCGGCGTGAATAACGCCGCGGTGGCCGTGGCGCCGCGCGCACCGTTGAACGTTGCGCGCCGTCCCGA
TCCGGTCACCGAGCCGCCCGTCGTACGCTGGATCCTGACCGGCGTGGCGCTGCTGTTTCTCGCGCTGTTCCTCGTCGTGC
CGCTCGTCGCCGTGTTCTATCAGGCGTTGAACAAGGGACTCGGTTTCTATCTGGAATCGCTTGCCGATCCCGACGCGCTC
TCGGCGATCAAGCTCACCGTGATCACCGCCGCGATCGCCGTGCCGCTGAACCTCGTGTTCGGCCTCGCGGCTTCATGGTG
TATCGCCAAATTCGAATTCCGCGGCAAGGCGCTGCTGACTACGCTGATCGATCTGCCGTTCTCGGTGTCGCCGGTGATCT
CGGGCCTGATCTATGTGCTGATGTTCGGCGCGCAGGGCTGGTTCGGGCCGTGGCTGCAGGATCACAACATCCAGATCATT
TTCGCGGTGCCGGGCATCGTGATGGCGACGATCTTCGTGACGTTCCCGTTTGTCGCGCGTGAACTGATTCCGCTGATGCA
GGCGCAAGGCAACGACGAAGAAGAAGCCGCGCACGTGCTCGGCGCGTCGGGCTGGCAGATCTTCCGCCGCGTCACGCTGC
CCAACGTCAAATGGGGCTTGCTGTACGGCGTGATTCTGTGCAACGCGCGGGCGATGGGCGAGTTCGGCGCAGTGTCGGTG
GTGTCGGGCCATATTCGCGGCCAGACCGACACCATGCCGCTGCACGTCGAAATTCTCTACAACGAATACAACTTCTCGGC
GGCGTTCGCCGTGGCGTCGGTGCTGGCGCTGCTCGCACTCGTGACGCTCGGCCTGAAGCTGCTCGCCGAGCGCCATATGT
CGGCGGAACTGTCGGCCGCGCGGGATGTGCCCGCGTACGCCGGTCCCATCACGCCGCCGGCCAGTGCAGCAGCAACAGCA
GCAACAGCAGCAACAGCAGCAACGGCGACACACGCACCGCATGTATCGCAGCAACACCCGCTCAAGCAAGGAGAGCTGTA
A

Upstream 100 bases:

>100_bases
GCACGAGCCGCGGCGGCGCGGGTCCGGCGCCTGCCGCCAGTGTTGCGGCCGTCGGCGGAGGTGTGCAATGAGCCGCGGCA
CCGCGAATGGAGCGGATGAC

Downstream 100 bases:

>100_bases
TGGGTATCACCGTTCGTAACCTGCAGAAGCGCTTCGGCGATTTCGTCGCGCTCGATAACGTCACGCTCGACTTTCCGCCG
GGTGAACTGGTTGCGCTGCT

Product: ABC sulfate/thiosulfate transporter, innermembrane subunit, CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNGVRGVNNAAVAVAPRAPLNVARRPDPVTEPPVVRWILTGVALLFLALFLVVPLVAVFYQALNKGLGFYLESLADPDAL
SAIKLTVITAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLQDHNIQII
FAVPGIVMATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSV
VSGHIRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELSAARDVPAYAGPITPPASAAATA
ATAATAATATHAPHVSQQHPLKQGEL

Sequences:

>Translated_346_residues
MNGVRGVNNAAVAVAPRAPLNVARRPDPVTEPPVVRWILTGVALLFLALFLVVPLVAVFYQALNKGLGFYLESLADPDAL
SAIKLTVITAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLQDHNIQII
FAVPGIVMATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSV
VSGHIRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELSAARDVPAYAGPITPPASAAATA
ATAATAATATHAPHVSQQHPLKQGEL
>Mature_346_residues
MNGVRGVNNAAVAVAPRAPLNVARRPDPVTEPPVVRWILTGVALLFLALFLVVPLVAVFYQALNKGLGFYLESLADPDAL
SAIKLTVITAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLQDHNIQII
FAVPGIVMATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSV
VSGHIRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELSAARDVPAYAGPITPPASAAATA
ATAATAATATHAPHVSQQHPLKQGEL

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=267, Percent_Identity=53.9325842696629, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=36.8932038834951, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=33.7016574585635, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36791; Mature: 36791

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGVRGVNNAAVAVAPRAPLNVARRPDPVTEPPVVRWILTGVALLFLALFLVVPLVAVFY
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALNKGLGFYLESLADPDALSAIKLTVITAAIAVPLNLVFGLAASWCIAKFEFRGKALLT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLQDHNIQIIFAVPGIVMATIFVTFPFVAR
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
ELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSV
HHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHH
VSGHIRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELSAA
EECCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDVPAYAGPITPPASAAATAATAATAATATHAPHVSQQHPLKQGEL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGVRGVNNAAVAVAPRAPLNVARRPDPVTEPPVVRWILTGVALLFLALFLVVPLVAVFY
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALNKGLGFYLESLADPDALSAIKLTVITAAIAVPLNLVFGLAASWCIAKFEFRGKALLT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLQDHNIQIIFAVPGIVMATIFVTFPFVAR
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
ELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSV
HHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHH
VSGHIRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELSAA
EECCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDVPAYAGPITPPASAAATAATAATAATATHAPHVSQQHPLKQGEL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]