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The map label for this gene is mhpE [C]

Identifier: 91782288

GI number: 91782288

Start: 1014623

End: 1015645

Strand: Direct

Name: mhpE [C]

Synonym: Bxe_A3546

Alternate gene names: 91782288

Gene position: 1014623-1015645 (Clockwise)

Preceding gene: 91782287

Following gene: 91782291

Centisome position: 20.72

GC content: 61.58

Gene sequence:

>1023_bases
ATGAATGAGAAGAGCAGGAAAATTTATGTCTCGGACGTGACCCTGCGTGATGGCATGCACGCCATCCGGCATCAGTACAG
TCTGGCCGATGTCGGGCGTATCGCCCGGGCGCTGGACCAGGCCGGGGTCGACAGTATCGAGGTTGCACACGGCGACGGTC
TGCGAGGCTCATCGTTCAACTACGGCTTTGGTGCCCATACGGATCTCGAGTGGATCGCTGCTGTTGCCGAAGTCGTGGAG
CAGGCGAAGATCGCGACGCTGCTTTTGCCTGGCATCGGCACGATCCATGACCTCAAGGCTGCCTATGACGCTGGCGCGCG
TGTCGTGCGCGTCGCAACGCACTGCACGGAGGCGGATATCTCCAGGCAGCACATCGAATATGCACGCTCTCTCGGGATGG
ATACCGTTGGCTTCCTGATGATGAGCCACATGACGACACCGGAGAACCTGGCTGTCGAGGCACGGAAGATGGAAAGCTAT
GGCGCGACCTGCATCTACGTGGTTGACTCGGGCGGAGCGATGAACATGAACGATATCCGGGACCGCTTTCGCGCGTTGAA
GGAGGCGCTCGATCCGGCGACGCAGACAGGTATCCACGCACATCACAATCTGAGTCTTGGCGTCGCGAATTCCATCGTCG
CTGTGGAAGAAGGCTGCGACCGGGTGGATGCGAGCCTCGCCGGTATGGGCGCGGGAGCGGGCAATGCACCACTGGAAGTG
TTCATCGCAGCAGCGGAACGGCTTGGCTGGAATCACGGGACCGACCTTTACGCCTTGATGGATGCCGCAGACGACATCGT
GCGACCGCTGCAGGACCGACCGGTGCGGGTCGATCGCGAGACGCTAGCGCTTGGCTATGCCGGCGTGTATTCCAGCTTCC
TTCGGCACTCGGAGGTCGCAGCCGACAGGTACGGCCTGAAGGCGGTGGATATTCTCGTCGAGCTGGGTCGCCGCAAGATG
GTGGGCGGGCAGGAGGACATGATCGTCGACGTGGCGCTTGACCTGCTGCGCAACCCTGCCTGA

Upstream 100 bases:

>100_bases
GCTATGCGGGGAACCTGGACATCATGACCAGCGCCGCGCTGGCTACGGCCGAGCGCATCGCGGCACGTCAGCCGGTGACG
GCTTAAGGAGGGAGTGGACC

Downstream 100 bases:

>100_bases
CATGGTGGTCTGCGGGGCATCGCGATGCGCCGGCCTGGTGATGCGGCAGCCAGGAGGCTATGTTGTGTGTGTATAACAGG
GAGGGGCGTGGCGGCTTCAG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 1; 4-hydroxy-2-keto-pentanoic acid aldolase 1; 4-hydroxy-2-oxopentanoate aldolase 1

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNEKSRKIYVSDVTLRDGMHAIRHQYSLADVGRIARALDQAGVDSIEVAHGDGLRGSSFNYGFGAHTDLEWIAAVAEVVE
QAKIATLLLPGIGTIHDLKAAYDAGARVVRVATHCTEADISRQHIEYARSLGMDTVGFLMMSHMTTPENLAVEARKMESY
GATCIYVVDSGGAMNMNDIRDRFRALKEALDPATQTGIHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEV
FIAAAERLGWNHGTDLYALMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVAADRYGLKAVDILVELGRRKM
VGGQEDMIVDVALDLLRNPA

Sequences:

>Translated_340_residues
MNEKSRKIYVSDVTLRDGMHAIRHQYSLADVGRIARALDQAGVDSIEVAHGDGLRGSSFNYGFGAHTDLEWIAAVAEVVE
QAKIATLLLPGIGTIHDLKAAYDAGARVVRVATHCTEADISRQHIEYARSLGMDTVGFLMMSHMTTPENLAVEARKMESY
GATCIYVVDSGGAMNMNDIRDRFRALKEALDPATQTGIHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEV
FIAAAERLGWNHGTDLYALMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVAADRYGLKAVDILVELGRRKM
VGGQEDMIVDVALDLLRNPA
>Mature_340_residues
MNEKSRKIYVSDVTLRDGMHAIRHQYSLADVGRIARALDQAGVDSIEVAHGDGLRGSSFNYGFGAHTDLEWIAAVAEVVE
QAKIATLLLPGIGTIHDLKAAYDAGARVVRVATHCTEADISRQHIEYARSLGMDTVGFLMMSHMTTPENLAVEARKMESY
GATCIYVVDSGGAMNMNDIRDRFRALKEALDPATQTGIHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEV
FIAAAERLGWNHGTDLYALMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVAADRYGLKAVDILVELGRRKM
VGGQEDMIVDVALDLLRNPA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=333, Percent_Identity=85.2852852852853, Blast_Score=565, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA1_BURXL (Q143P7)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_557494.1
- ProteinModelPortal:   Q143P7
- SMR:   Q143P7
- GeneID:   4004911
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A3546
- HOGENOM:   HBG300122
- OMA:   LMALMDA
- ProtClustDB:   PRK08195
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36714; Mature: 36714

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50991 PYR_CT; PS00260 GLUCAGON

Important sites: BINDING 170-170 BINDING 199-199 BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKSRKIYVSDVTLRDGMHAIRHQYSLADVGRIARALDQAGVDSIEVAHGDGLRGSSFN
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
YGFGAHTDLEWIAAVAEVVEQAKIATLLLPGIGTIHDLKAAYDAGARVVRVATHCTEADI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHCCHHHH
SRQHIEYARSLGMDTVGFLMMSHMTTPENLAVEARKMESYGATCIYVVDSGGAMNMNDIR
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
DRFRALKEALDPATQTGIHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEV
HHHHHHHHHHCHHHHCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
FIAAAERLGWNHGTDLYALMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHH
ADRYGLKAVDILVELGRRKMVGGQEDMIVDVALDLLRNPA
HHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEKSRKIYVSDVTLRDGMHAIRHQYSLADVGRIARALDQAGVDSIEVAHGDGLRGSSFN
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
YGFGAHTDLEWIAAVAEVVEQAKIATLLLPGIGTIHDLKAAYDAGARVVRVATHCTEADI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHCCHHHH
SRQHIEYARSLGMDTVGFLMMSHMTTPENLAVEARKMESYGATCIYVVDSGGAMNMNDIR
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
DRFRALKEALDPATQTGIHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEV
HHHHHHHHHHCHHHHCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
FIAAAERLGWNHGTDLYALMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHH
ADRYGLKAVDILVELGRRKMVGGQEDMIVDVALDLLRNPA
HHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA