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The map label for this gene is gmd [H]

Identifier: 91781981

GI number: 91781981

Start: 673991

End: 675109

Strand: Direct

Name: gmd [H]

Synonym: Bxe_A3863

Alternate gene names: 91781981

Gene position: 673991-675109 (Clockwise)

Preceding gene: 91781980

Following gene: 91781982

Centisome position: 13.77

GC content: 62.65

Gene sequence:

>1119_bases
ATGAAACGTAAGGTCGCGCTGATCACCGGCATCACAGGACAGGACGGGTCGTATCTGGCGGAGTTGCTGCTCGCCAAGGA
TTACGACGTACACGGCATCAAACGCAGGTCATCCCTGTTCAATACAGACCGGATCGATCACCTGTACCGCGATCCTCACG
ATCCGGACCAGCGGCTCTTTCTGCATCACGCGGATCTGACCGACTCGACCAGCATTCTGCGCGTGATCCAGCGTGTCGAG
CCCGACGAGATCTACAACCTCGCCGCGCAGAGCCACGTCGCGGTGTCGTTCGAGGAGCCGGAATACACCGCGAACGCCGA
CGGCCTCGGCGCGTTGCGGATTCTCGAAGCGATCCGGATTCTCGGGCTGCAGGAAAAGACGCGCTTCTATCAGGCGTCCA
CCTCGGAGCTTTACGGCCTCGTGCAGCAGGTGCCGCAGTCGGAGACCACGCCGTTCTATCCGCGCAGCCCGTATGCCGTC
GCCAAGCTGTTCGCGTACTGGACCACGGTCAATTATCGCGAGGCTTACGGTCTCTATGCGTGCAACGGGATTCTGTTCAA
TCACGAATCGCCGGTGCGCGGCGAGACGTTCGTGACGCGCAAGATTACGCGCGCAGTCGCGCGTATCGCGGTGGGCATGC
AGAAGACCCTGTATCTCGGCAATCTGTCGGCCTTGCGCGACTGGGGCCACGCGCGCGATTACGTGGAGATGCAATGGCGC
ATGCTCCAGCAGGAGCAGCCGGAGGATTACGTGATCGCCACCGGCGTGCAGTACAGCGTGCGCCAGTTCGTGCAGCACGC
GGCCGCCGAGCTCGGCGTCACCGTGCGCTTCGAAGGCACCGGCGTGGATGAAATCGGCATTGTCGAGAAAGTGGAAGGGC
GCGAGATCAAGATGTCGCCGGGCGACGTGATCGTGCGTGTCGATCCGCGCTACTTCCGGCCCGCCGAAGTCGAGACGCTG
CTCGGCGATCCGTCCAAGGCGCATGCGAAGCTCGGCTGGCAGCCGACCACGTCGTTCGCTTCACTGGTCAAGGAAATGGT
GCGCGCGGACTATCAGATTGCCCGACGTGACGCGCTCGTCACGCTGGCCGGCTTCACGGCGCTGGAACATCACGAGTAA

Upstream 100 bases:

>100_bases
CAGCATCGCCGGTTGACATGGCGGCGGATAGCTGATGGTCAGACGAGGTTCAACGAGGTTGCGGCATCAGGCTTGCAAAG
GTTAACGAGGAGAATGCTCC

Downstream 100 bases:

>100_bases
CCTGCGATGAACAAACAAGCACGCATTTTCGTCGCGGGCCACCGGGGCATGGTCGGCTCCGCGCTCGTCAGGCGCCTTGC
CGCCGACGGGTATCAGAACG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKRKVALITGITGQDGSYLAELLLAKDYDVHGIKRRSSLFNTDRIDHLYRDPHDPDQRLFLHHADLTDSTSILRVIQRVE
PDEIYNLAAQSHVAVSFEEPEYTANADGLGALRILEAIRILGLQEKTRFYQASTSELYGLVQQVPQSETTPFYPRSPYAV
AKLFAYWTTVNYREAYGLYACNGILFNHESPVRGETFVTRKITRAVARIAVGMQKTLYLGNLSALRDWGHARDYVEMQWR
MLQQEQPEDYVIATGVQYSVRQFVQHAAAELGVTVRFEGTGVDEIGIVEKVEGREIKMSPGDVIVRVDPRYFRPAEVETL
LGDPSKAHAKLGWQPTTSFASLVKEMVRADYQIARRDALVTLAGFTALEHHE

Sequences:

>Translated_372_residues
MKRKVALITGITGQDGSYLAELLLAKDYDVHGIKRRSSLFNTDRIDHLYRDPHDPDQRLFLHHADLTDSTSILRVIQRVE
PDEIYNLAAQSHVAVSFEEPEYTANADGLGALRILEAIRILGLQEKTRFYQASTSELYGLVQQVPQSETTPFYPRSPYAV
AKLFAYWTTVNYREAYGLYACNGILFNHESPVRGETFVTRKITRAVARIAVGMQKTLYLGNLSALRDWGHARDYVEMQWR
MLQQEQPEDYVIATGVQYSVRQFVQHAAAELGVTVRFEGTGVDEIGIVEKVEGREIKMSPGDVIVRVDPRYFRPAEVETL
LGDPSKAHAKLGWQPTTSFASLVKEMVRADYQIARRDALVTLAGFTALEHHE
>Mature_372_residues
MKRKVALITGITGQDGSYLAELLLAKDYDVHGIKRRSSLFNTDRIDHLYRDPHDPDQRLFLHHADLTDSTSILRVIQRVE
PDEIYNLAAQSHVAVSFEEPEYTANADGLGALRILEAIRILGLQEKTRFYQASTSELYGLVQQVPQSETTPFYPRSPYAV
AKLFAYWTTVNYREAYGLYACNGILFNHESPVRGETFVTRKITRAVARIAVGMQKTLYLGNLSALRDWGHARDYVEMQWR
MLQQEQPEDYVIATGVQYSVRQFVQHAAAELGVTVRFEGTGVDEIGIVEKVEGREIKMSPGDVIVRVDPRYFRPAEVETL
LGDPSKAHAKLGWQPTTSFASLVKEMVRADYQIARRDALVTLAGFTALEHHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=356, Percent_Identity=57.3033707865169, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1788366, Length=363, Percent_Identity=69.4214876033058, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI48994969, Length=368, Percent_Identity=25.5434782608696, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=23.2686980609418, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=360, Percent_Identity=61.6666666666667, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539424, Length=359, Percent_Identity=60.4456824512535, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901790, Length=359, Percent_Identity=60.4456824512535, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17539422, Length=359, Percent_Identity=60.4456824512535, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901788, Length=359, Percent_Identity=60.4456824512535, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901786, Length=359, Percent_Identity=60.4456824512535, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24158427, Length=357, Percent_Identity=57.9831932773109, Blast_Score=438, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 42136; Mature: 42136

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVALITGITGQDGSYLAELLLAKDYDVHGIKRRSSLFNTDRIDHLYRDPHDPDQRLF
CCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEE
LHHADLTDSTSILRVIQRVEPDEIYNLAAQSHVAVSFEEPEYTANADGLGALRILEAIRI
EEECCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LGLQEKTRFYQASTSELYGLVQQVPQSETTPFYPRSPYAVAKLFAYWTTVNYREAYGLYA
HCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCEEE
CNGILFNHESPVRGETFVTRKITRAVARIAVGMQKTLYLGNLSALRDWGHARDYVEMQWR
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHH
MLQQEQPEDYVIATGVQYSVRQFVQHAAAELGVTVRFEGTGVDEIGIVEKVEGREIKMSP
HHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCEEEECC
GDVIVRVDPRYFRPAEVETLLGDPSKAHAKLGWQPTTSFASLVKEMVRADYQIARRDALV
CCEEEEECCCCCCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEE
TLAGFTALEHHE
EEECHHHHCCCC
>Mature Secondary Structure
MKRKVALITGITGQDGSYLAELLLAKDYDVHGIKRRSSLFNTDRIDHLYRDPHDPDQRLF
CCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEE
LHHADLTDSTSILRVIQRVEPDEIYNLAAQSHVAVSFEEPEYTANADGLGALRILEAIRI
EEECCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LGLQEKTRFYQASTSELYGLVQQVPQSETTPFYPRSPYAVAKLFAYWTTVNYREAYGLYA
HCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCEEE
CNGILFNHESPVRGETFVTRKITRAVARIAVGMQKTLYLGNLSALRDWGHARDYVEMQWR
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHH
MLQQEQPEDYVIATGVQYSVRQFVQHAAAELGVTVRFEGTGVDEIGIVEKVEGREIKMSP
HHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCEEEECC
GDVIVRVDPRYFRPAEVETLLGDPSKAHAKLGWQPTTSFASLVKEMVRADYQIARRDALV
CCEEEEECCCCCCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEE
TLAGFTALEHHE
EEECHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]