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The map label for this gene is fcl [H]

Identifier: 91781982

GI number: 91781982

Start: 675116

End: 676075

Strand: Direct

Name: fcl [H]

Synonym: Bxe_A3862

Alternate gene names: 91781982

Gene position: 675116-676075 (Clockwise)

Preceding gene: 91781981

Following gene: 91781983

Centisome position: 13.79

GC content: 63.65

Gene sequence:

>960_bases
ATGAACAAACAAGCACGCATTTTCGTCGCGGGCCACCGGGGCATGGTCGGCTCCGCGCTCGTCAGGCGCCTTGCCGCCGA
CGGGTATCAGAACGTGATCACCCGTTCGCGCCAGGAGCTCGACCTGACGGATCAGGGCGCAGTGAACCGCTTCTTCGAAA
GCGAGAGGATCGACGTGGTGCTGCTCGCGGCCGCGCGGGTGGGCGGCATTCTCGCGAACGCCACGCGGCCGGGTGAGTTC
ATCTATGAAAATCTGGTGATCGAAACCAACGTGATTCACGCGGCGTACCGTGCCAGGGTCGAGCGGCTGGTGTTCTTCGG
TTCCTCGTGCATTTATCCGAAGCAATGTCCGCAGCCGATCCGCGAGGAGTATCTGCTGACCTCGCCGCTCGAACCGACCA
ACGACGCCTACGCGATCGCGAAGATCGCCGGCGTCAAGCTGTGCGAAGCCTACAACCGCGAGTACAACACCCAGTACGTC
GCGCTGATGCCGACCAATCTGTATGGCCCGAACGACAACTACGACCTGAACAGCAGCCACGTGTTGCCGGCGCTGCTGCG
CAAGGCGCACGAAGCGAAGCTGAACGGCGACGCCACGTTGTCGGTGTGGGGTTCGGGCACGCCGCGCCGCGAGTTCCTGC
ATGTCGACGATCTCGCCGCGGCCACGCTGTTCGTGCTCGAACACAACGTGACAGAGGGGCTCTTCAACGTCGGCGTGGGC
AAGGACCTGTCGATTCGCGAACTGGCCGAATGCATCTGCAAGGTGGCCGGCTTCGACGGTGAGCTCATGTTCGACGCCTC
GAAACCCGACGGCACGCCGCGCAAGCTGCTCGACGTCTCGCGGCTCGCGCAAATGGGCTGGCAGGCGAGCATTGCGCTGG
AGGACGGTATCGCATCCACGTACCGGGATTTCGTCGAATCACACGCCGGTTCGACGCCCGCTGCCGCCGTCGAGGTTTAG

Upstream 100 bases:

>100_bases
GGTCAAGGAAATGGTGCGCGCGGACTATCAGATTGCCCGACGTGACGCGCTCGTCACGCTGGCCGGCTTCACGGCGCTGG
AACATCACGAGTAACCTGCG

Downstream 100 bases:

>100_bases
CCGCACGAATTGCCGCTTCAGCGCGGGTTGGCAGTCAAGGACCAGCTGGGACGCCGTGCATGCGCACCCACGCGCGATGC
ACGCAGGACGATTTTTCGAG

Product: putative nucleoside-diphosphate-sugar epimerases

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNKQARIFVAGHRGMVGSALVRRLAADGYQNVITRSRQELDLTDQGAVNRFFESERIDVVLLAAARVGGILANATRPGEF
IYENLVIETNVIHAAYRARVERLVFFGSSCIYPKQCPQPIREEYLLTSPLEPTNDAYAIAKIAGVKLCEAYNREYNTQYV
ALMPTNLYGPNDNYDLNSSHVLPALLRKAHEAKLNGDATLSVWGSGTPRREFLHVDDLAAATLFVLEHNVTEGLFNVGVG
KDLSIRELAECICKVAGFDGELMFDASKPDGTPRKLLDVSRLAQMGWQASIALEDGIASTYRDFVESHAGSTPAAAVEV

Sequences:

>Translated_319_residues
MNKQARIFVAGHRGMVGSALVRRLAADGYQNVITRSRQELDLTDQGAVNRFFESERIDVVLLAAARVGGILANATRPGEF
IYENLVIETNVIHAAYRARVERLVFFGSSCIYPKQCPQPIREEYLLTSPLEPTNDAYAIAKIAGVKLCEAYNREYNTQYV
ALMPTNLYGPNDNYDLNSSHVLPALLRKAHEAKLNGDATLSVWGSGTPRREFLHVDDLAAATLFVLEHNVTEGLFNVGVG
KDLSIRELAECICKVAGFDGELMFDASKPDGTPRKLLDVSRLAQMGWQASIALEDGIASTYRDFVESHAGSTPAAAVEV
>Mature_319_residues
MNKQARIFVAGHRGMVGSALVRRLAADGYQNVITRSRQELDLTDQGAVNRFFESERIDVVLLAAARVGGILANATRPGEF
IYENLVIETNVIHAAYRARVERLVFFGSSCIYPKQCPQPIREEYLLTSPLEPTNDAYAIAKIAGVKLCEAYNREYNTQYV
ALMPTNLYGPNDNYDLNSSHVLPALLRKAHEAKLNGDATLSVWGSGTPRREFLHVDDLAAATLFVLEHNVTEGLFNVGVG
KDLSIRELAECICKVAGFDGELMFDASKPDGTPRKLLDVSRLAQMGWQASIALEDGIASTYRDFVESHAGSTPAAAVEV

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=283, Percent_Identity=35.6890459363958, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=23.7951807228916, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=25.6329113924051, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=58.5443037974684, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=22.1574344023324, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554428, Length=310, Percent_Identity=31.6129032258064, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI115532424, Length=277, Percent_Identity=24.187725631769, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922778, Length=315, Percent_Identity=29.2063492063492, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35022; Mature: 35022

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQARIFVAGHRGMVGSALVRRLAADGYQNVITRSRQELDLTDQGAVNRFFESERIDVV
CCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCCHHHCCCCCCHHHHHHHHCCCCCEE
LLAAARVGGILANATRPGEFIYENLVIETNVIHAAYRARVERLVFFGSSCIYPKQCPQPI
EEEHHHHCCEEECCCCCHHHHHHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHH
REEYLLTSPLEPTNDAYAIAKIAGVKLCEAYNREYNTQYVALMPTNLYGPNDNYDLNSSH
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
VLPALLRKAHEAKLNGDATLSVWGSGTPRREFLHVDDLAAATLFVLEHNVTEGLFNVGVG
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHEEEHHHHHHHHHHHHHCCHHHHHHHCCCC
KDLSIRELAECICKVAGFDGELMFDASKPDGTPRKLLDVSRLAQMGWQASIALEDGIAST
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHH
YRDFVESHAGSTPAAAVEV
HHHHHHHHCCCCCCCEECC
>Mature Secondary Structure
MNKQARIFVAGHRGMVGSALVRRLAADGYQNVITRSRQELDLTDQGAVNRFFESERIDVV
CCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCCHHHCCCCCCHHHHHHHHCCCCCEE
LLAAARVGGILANATRPGEFIYENLVIETNVIHAAYRARVERLVFFGSSCIYPKQCPQPI
EEEHHHHCCEEECCCCCHHHHHHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHH
REEYLLTSPLEPTNDAYAIAKIAGVKLCEAYNREYNTQYVALMPTNLYGPNDNYDLNSSH
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
VLPALLRKAHEAKLNGDATLSVWGSGTPRREFLHVDDLAAATLFVLEHNVTEGLFNVGVG
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHEEEHHHHHHHHHHHHHCCHHHHHHHCCCC
KDLSIRELAECICKVAGFDGELMFDASKPDGTPRKLLDVSRLAQMGWQASIALEDGIAST
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHH
YRDFVESHAGSTPAAAVEV
HHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]