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The map label for this gene is ygfP [H]

Identifier: 91212261

GI number: 91212261

Start: 3204313

End: 3205752

Strand: Direct

Name: ygfP [H]

Synonym: UTI89_C3268

Alternate gene names: 91212261

Gene position: 3204313-3205752 (Clockwise)

Preceding gene: 91212260

Following gene: 91212262

Centisome position: 63.25

GC content: 48.19

Gene sequence:

>1440_bases
TTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA
TTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCA
GTTTTATTGATGTCACCCGTACGGTCGATAACCCGGAAGAAATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTA
CTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAGACGGAAAGCATCAAATTCCTGACACCATTCGCGTGCG
CGACTATCGCGGCAAACTGATAGTACCGGGCTTTATCGATACACATATCCATTATCCGCAAAGTGAAATGGTGGGGGCCT
ATGGTGAACAATTGCTGGAGTGGTTGAATAAACACACCTTCCCTACTGAACGTCGTTATGAGGATTTAGAGTACGCCCGC
GAGATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCACA
ATCCGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAAGTGATGATGGACCGAAACG
CACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATTGAACGCTGGCACAAAAATGGT
CGTCTGTTGTATGCGATTACGCCACGCTTCGCCCCTACCTCATCTCCTGAACAGATGGCGATGGCGCAACGCCTGAAAGA
AGAATATCCGGATACATGGGTACATACCCATCTCTGTGAAAACAAAGATGAAATTGCCTGGGTGAAATCGCTTTATCCTG
ACCATGATGGTTATCTTGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGCGTCTTTGCTCACTGCGTCCATCTC
GAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAG
CGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTCAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACAACTT
TTAACATGCTGCAAACGCTGAACGAAGCCTACAAGGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTT
TACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGA
TTTCGTGGTGATGGAACCCACTGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGT
TCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAA

Upstream 100 bases:

>100_bases
TTGTCGCCACTTCTTTAGGTTTAGGACTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTAT
GTATTAGTTGAAAACCCTAT

Downstream 100 bases:

>100_bases
TAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAG
GGCGCGCTGGATAATTATTT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MCWRVNCDFIKYYPPWWLPTGKRSAWYYLSGRDGLTVKESMMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLL
LIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAR
EMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNG
RLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHL
EEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAF
YLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Sequences:

>Translated_479_residues
MCWRVNCDFIKYYPPWWLPTGKRSAWYYLSGRDGLTVKESMMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLL
LIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAR
EMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNG
RLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHL
EEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAF
YLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN
>Mature_479_residues
MCWRVNCDFIKYYPPWWLPTGKRSAWYYLSGRDGLTVKESMMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLL
LIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAR
EMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNG
RLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHL
EEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAF
YLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=381, Percent_Identity=36.4829396325459, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1789249, Length=439, Percent_Identity=99.3166287015945, Blast_Score=914, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319963, Length=352, Percent_Identity=34.9431818181818, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24643849, Length=399, Percent_Identity=33.3333333333333, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 55102; Mature: 55102

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCWRVNCDFIKYYPPWWLPTGKRSAWYYLSGRDGLTVKESMMSGEHTLKAVRGSFIDVTR
CEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHCCCEEEEE
TVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFID
CCCCHHHHHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCCCEEEEEECCCCEEECCEEC
THIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALV
CCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNG
EECCCCHHHHHHHHHHHHCCEEEEECCCEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCC
RLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLD
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCH
VYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKV
HHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCEEECCCCCEEECCCHHHHHHHHHHHH
KVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNF
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHEEEEEECCCCCCCCHHHHHHCC
LPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN
CCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHEEEEECCCCCEEHEEECCEEEEECC
>Mature Secondary Structure
MCWRVNCDFIKYYPPWWLPTGKRSAWYYLSGRDGLTVKESMMSGEHTLKAVRGSFIDVTR
CEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHCCCEEEEE
TVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFID
CCCCHHHHHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCCCEEEEEECCCCEEECCEEC
THIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALV
CCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNG
EECCCCHHHHHHHHHHHHCCEEEEECCCEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCC
RLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLD
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCH
VYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKV
HHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCEEECCCCCEEECCCHHHHHHHHHHHH
KVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNF
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHEEEEEECCCCCCCCHHHHHHCC
LPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN
CCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHEEEEECCCCCEEHEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]