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The map label for this gene is prfA [H]

Identifier: 89898158

GI number: 89898158

Start: 414453

End: 415538

Strand: Direct

Name: prfA [H]

Synonym: CF0351

Alternate gene names: 89898158

Gene position: 414453-415538 (Clockwise)

Preceding gene: 89898157

Following gene: 89898159

Centisome position: 35.54

GC content: 41.8

Gene sequence:

>1086_bases
ATGGAAAAAAAGATTCTAGAGTATTTGAAGCGCCTAGAGGAAGTGGAAGTTAAGATTTCTGATCCGGAAATTTTTAATAA
TCCTAAAGAGTATAGTAGTTTGAGTAGGGAGCACGCACGTCTTACTGAATTAAAAAACGTGTATGATAAGGTTTTAAGAC
ACGAAAAAATTCTCAATGATGATAAACATGCTCTGGCTCAAGAAAAAGATCCTGAGATGATTGCAATGCTAGAAGAGGGT
ATTCAATCAGGAAAATCTGAAATTGAAAAGCTTTATAAAATTTTAGAAAATTTATTGGTGCCACCCGATCCAGATGATGA
TTTAAATGTGATCATGGAATTGCGCGCGGGTACAGGAGGAGATGAGGCGGCCCTGTTTGTGGGGGATTGTGTGCGGATGT
ATCATCTGTATGCTTCTGCTAAAGGATGGAAATATGAAGAGCTTTCAGCTTCTGAATCTGATATCGGCGGATATAAAGAA
TATGTCATGGGTATCTCAGGAACTGGGGTAAAGCGCTTGCTTCAGTATGAAGCGGGAACTCACCGAGTTCAAAGGGTCCC
CGAAACCGAAACTCAAGGACGTGTTCATACGTCTGCGATTACTGTGGCCGTATTGCCAGAACCAGCAGAAGACGATGAAG
AAGTCTTTGTCGATGAGAAAGATTTAAAAATAGACACTTTTAGAGCTTCCGGCGCTGGCGGCCAGCACGTAAACGTTACA
GACTCAGCAGTAAGAATTACTCACTTGCCTACGGGTGTTGTTGTTACTTGTCAGGATGAACGTAGTCAGCATAAAAATAA
AGCCAAAGCTATGCGTATCTTAAAGGCTCGTATACGTGATGCTGAGATGCAAAGACGTCATAAAGAAGCTTCGGCTATGC
GCTCGGCTCAGGTGGGTAGTGGAGATCGATCGGAAAGAATTCGCACCTATAACTTCTCGCAAAATCGCGTGACCGATCAT
AGAATAGGCCTTACTCTGTACAATTTAGATAAAGTTATGGAAGGGGATTTAGACACCATTACTTCAGCATTAGTTAGCCA
CGCTTATCACCAGCTCTTTCAAAATGGAAACGAAGAGAATTCTTAG

Upstream 100 bases:

>100_bases
TGGCGGCTACGAGCCCCCAGAAGTTAAATAATTTGAATATAAGAGCTTCTCGTTCTCAAGTCGAGAAGTTTGTCAGAAAC
TCTCGATTGAGAAGTGGTGC

Downstream 100 bases:

>100_bases
CAAAGCTTCTGCATATTTAAAATATCATGGCGTTGCTTTCTCCGATAGAGAGGCTTCTGATATTTTGATGGATTTTCTTG
GAATAAGTTCTCGGGCGCAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MEKKILEYLKRLEEVEVKISDPEIFNNPKEYSSLSREHARLTELKNVYDKVLRHEKILNDDKHALAQEKDPEMIAMLEEG
IQSGKSEIEKLYKILENLLVPPDPDDDLNVIMELRAGTGGDEAALFVGDCVRMYHLYASAKGWKYEELSASESDIGGYKE
YVMGISGTGVKRLLQYEAGTHRVQRVPETETQGRVHTSAITVAVLPEPAEDDEEVFVDEKDLKIDTFRASGAGGQHVNVT
DSAVRITHLPTGVVVTCQDERSQHKNKAKAMRILKARIRDAEMQRRHKEASAMRSAQVGSGDRSERIRTYNFSQNRVTDH
RIGLTLYNLDKVMEGDLDTITSALVSHAYHQLFQNGNEENS

Sequences:

>Translated_361_residues
MEKKILEYLKRLEEVEVKISDPEIFNNPKEYSSLSREHARLTELKNVYDKVLRHEKILNDDKHALAQEKDPEMIAMLEEG
IQSGKSEIEKLYKILENLLVPPDPDDDLNVIMELRAGTGGDEAALFVGDCVRMYHLYASAKGWKYEELSASESDIGGYKE
YVMGISGTGVKRLLQYEAGTHRVQRVPETETQGRVHTSAITVAVLPEPAEDDEEVFVDEKDLKIDTFRASGAGGQHVNVT
DSAVRITHLPTGVVVTCQDERSQHKNKAKAMRILKARIRDAEMQRRHKEASAMRSAQVGSGDRSERIRTYNFSQNRVTDH
RIGLTLYNLDKVMEGDLDTITSALVSHAYHQLFQNGNEENS
>Mature_361_residues
MEKKILEYLKRLEEVEVKISDPEIFNNPKEYSSLSREHARLTELKNVYDKVLRHEKILNDDKHALAQEKDPEMIAMLEEG
IQSGKSEIEKLYKILENLLVPPDPDDDLNVIMELRAGTGGDEAALFVGDCVRMYHLYASAKGWKYEELSASESDIGGYKE
YVMGISGTGVKRLLQYEAGTHRVQRVPETETQGRVHTSAITVAVLPEPAEDDEEVFVDEKDLKIDTFRASGAGGQHVNVT
DSAVRITHLPTGVVVTCQDERSQHKNKAKAMRILKARIRDAEMQRRHKEASAMRSAQVGSGDRSERIRTYNFSQNRVTDH
RIGLTLYNLDKVMEGDLDTITSALVSHAYHQLFQNGNEENS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=312, Percent_Identity=46.1538461538462, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI34577120, Length=332, Percent_Identity=41.2650602409639, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI166795305, Length=212, Percent_Identity=45.2830188679245, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI1787462, Length=349, Percent_Identity=53.0085959885387, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=36.3905325443787, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=300, Percent_Identity=36.6666666666667, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=320, Percent_Identity=42.5, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI19921226, Length=311, Percent_Identity=44.0514469453376, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40798; Mature: 40798

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKILEYLKRLEEVEVKISDPEIFNNPKEYSSLSREHARLTELKNVYDKVLRHEKILND
CCHHHHHHHHHHHHHEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKHALAQEKDPEMIAMLEEGIQSGKSEIEKLYKILENLLVPPDPDDDLNVIMELRAGTGG
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCCCC
DEAALFVGDCVRMYHLYASAKGWKYEELSASESDIGGYKEYVMGISGTGVKRLLQYEAGT
CCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCH
HRVQRVPETETQGRVHTSAITVAVLPEPAEDDEEVFVDEKDLKIDTFRASGAGGQHVNVT
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCEEEEEECCCCCCCEEECC
DSAVRITHLPTGVVVTCQDERSQHKNKAKAMRILKARIRDAEMQRRHKEASAMRSAQVGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDRSERIRTYNFSQNRVTDHRIGLTLYNLDKVMEGDLDTITSALVSHAYHQLFQNGNEEN
CCCCCCEEEECCCCCCCCHHHHCEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
S
C
>Mature Secondary Structure
MEKKILEYLKRLEEVEVKISDPEIFNNPKEYSSLSREHARLTELKNVYDKVLRHEKILND
CCHHHHHHHHHHHHHEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKHALAQEKDPEMIAMLEEGIQSGKSEIEKLYKILENLLVPPDPDDDLNVIMELRAGTGG
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCCCC
DEAALFVGDCVRMYHLYASAKGWKYEELSASESDIGGYKEYVMGISGTGVKRLLQYEAGT
CCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCH
HRVQRVPETETQGRVHTSAITVAVLPEPAEDDEEVFVDEKDLKIDTFRASGAGGQHVNVT
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCEEEEEECCCCCCCEEECC
DSAVRITHLPTGVVVTCQDERSQHKNKAKAMRILKARIRDAEMQRRHKEASAMRSAQVGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDRSERIRTYNFSQNRVTDHRIGLTLYNLDKVMEGDLDTITSALVSHAYHQLFQNGNEEN
CCCCCCEEEECCCCCCCCHHHHCEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA