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The map label for this gene is potF [H]

Identifier: 89256859

GI number: 89256859

Start: 1508981

End: 1510162

Strand: Reverse

Name: potF [H]

Synonym: FTL_1582

Alternate gene names: 89256859

Gene position: 1510162-1508981 (Counterclockwise)

Preceding gene: 89256865

Following gene: 89256856

Centisome position: 79.65

GC content: 30.03

Gene sequence:

>1182_bases
ATGAAACGAAAGGTTTTTATTATTGGCCTGCTAGTTAGTTTGTTTAATCTGGCATATTCAAAAGATGAGTCATATATTTG
TGATAATAAAATTCTTAAAAGTCCTATCACTGCTACACAAACTAGAACTCAATTAAATTTTACTAACTGGGCAGACTATA
TATCTCCATATATAGTACCATGTTTTTCAGACTTATCTAATACTAGAGTCAAGTATATCTATACTTCTGATGATAATATG
ACTCGTGCGAAAATTATGACAGGATCATCTGGTTTTGATTTAATTGAGCAAGGTGCATTATATTTAAATAGTGAAATCGC
ATCTAATGCTCTTGTTAAATTAGATAAATCAAAATTACCAAATCTAAAATATCGTAATAAAGTAATTTATGATAAAGTTT
CACAGATAAATGATCCAGGTAATAATTATGCTGTGGTATATAGTTATGGAACTACAGGTTTAGCTTATAACAAACAAGAA
ATAGAGCAAAGACTAGGTAAAGGTGTGGTTCCTAATAGCTGGAAATATGTTTTTGATAAAAAATACCTCAAACAAATAGC
TCCATGTGGAGTTTCGTTACTTGATGAACCTGAGCAAATATTTGGTAATTATTTTTTTTATCACGGAATTGATCCAAATA
CCAATAGCAAGGCTGAATATGAAAAAGCAGCTTTAGATATTATTAAAAATGTCCGCCCATATATTAAATATTTTGATAGT
AATAAATATCAAAATGATTTTACCGCGGGTAATCTTTGTTTAGTTATGGGTTATTCTGGTGATGTAGTTCGATCTGTTGA
AAGAGCTAAGTCAGTAAATCCGGATGTCACACTTGCATATGTTATTCCTAAAGAGGGTACTAATATTTGGTTTGATATGC
TAATGATACCAAAAGGTGCTAAAGATTTAGATAAAGCTTATGCTTTGATGAACTATATTATAGATCCATATGTATCAGCA
CAAAACAGTAATTATTTGTATCAGCCAAATGCTGTTACGCAAAACAAAAAATATCTAGATGATATCTTTGATGATACTAA
TATCAGACCTACTGATGAGATGATTAAGAAAATGTACGTTCTTAATATCCATGATGCCAAAATGCAGAGTTTTATTAGTA
GAATGTGGATGAATGTTAAGTACGGTATAGAATTTACACCAAAGTATTATAAGCCGCAATAA

Upstream 100 bases:

>100_bases
CTAATAATTTGTTCCATTATTTTACTTTTTATTTCTTCAATACCACGCTTTAGTATATACTTAAAGGAATTTTATAACAA
CTAGAGCGTGTGTTACTTTT

Downstream 100 bases:

>100_bases
AATAAGTAGCTTATTGTAGATTAGCCGTACAAACTAAACTTAACTTCTAAAATCAAAATCTCAAAATTTATGAGAGTAAA
TTCAAACTATAATAAAAATC

Product: putrescine-binding periplasmic protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKRKVFIIGLLVSLFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPYIVPCFSDLSNTRVKYIYTSDDNM
TRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLAYNKQE
IEQRLGKGVVPNSWKYVFDKKYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDS
NKYQNDFTAGNLCLVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYIIDPYVSA
QNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ

Sequences:

>Translated_393_residues
MKRKVFIIGLLVSLFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPYIVPCFSDLSNTRVKYIYTSDDNM
TRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLAYNKQE
IEQRLGKGVVPNSWKYVFDKKYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDS
NKYQNDFTAGNLCLVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYIIDPYVSA
QNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ
>Mature_393_residues
MKRKVFIIGLLVSLFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPYIVPCFSDLSNTRVKYIYTSDDNM
TRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLAYNKQE
IEQRLGKGVVPNSWKYVFDKKYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDS
NKYQNDFTAGNLCLVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYIIDPYVSA
QNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=347, Percent_Identity=32.8530259365994, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1787367, Length=315, Percent_Identity=28.2539682539683, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45215; Mature: 45215

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVFIIGLLVSLFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPYIVP
CCCEEEHHHHHHHHHHHHHCCCCCEECCCHHHCCCCCCCCCCEEECCCHHHHHHHHHHHH
CFSDLSNTRVKYIYTSDDNMTRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLP
HHHCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHCCCCEECHHHHCCHHEEECHHHCC
NLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLAYNKQEIEQRLGKGVVPNSWKYVFDK
CCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEECHHHHHHHHCCCCCCCCCHHHHHH
KYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDS
HHHHHHCCCCCHHCCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NKYQNDFTAGNLCLVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGA
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCC
KDLDKAYALMNYIIDPYVSAQNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYV
HHHHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHEE
LNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ
EEECHHHHHHHHHHHHCCEEECEEECCCCCCCC
>Mature Secondary Structure
MKRKVFIIGLLVSLFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPYIVP
CCCEEEHHHHHHHHHHHHHCCCCCEECCCHHHCCCCCCCCCCEEECCCHHHHHHHHHHHH
CFSDLSNTRVKYIYTSDDNMTRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLP
HHHCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHCCCCEECHHHHCCHHEEECHHHCC
NLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLAYNKQEIEQRLGKGVVPNSWKYVFDK
CCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEECHHHHHHHHCCCCCCCCCHHHHHH
KYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDS
HHHHHHCCCCCHHCCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NKYQNDFTAGNLCLVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGA
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCC
KDLDKAYALMNYIIDPYVSAQNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYV
HHHHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHEE
LNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ
EEECHHHHHHHHHHHHCCEEECEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]