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The map label for this gene is purD [H]

Identifier: 89055793

GI number: 89055793

Start: 3356155

End: 3357423

Strand: Direct

Name: purD [H]

Synonym: Jann_3302

Alternate gene names: 89055793

Gene position: 3356155-3357423 (Clockwise)

Preceding gene: 89055789

Following gene: 89055795

Centisome position: 77.73

GC content: 63.75

Gene sequence:

>1269_bases
ATGCATTTGAACATCCTTATCCTTGGCTCCGGAGGGCGCGAACATAGCCTGGCCTGGGCCGTTCTGCAGAACCCCAAATG
CGACCGCCTGATCGTTGCGCCCGGCAATGCGGGTATTGATCAGATCGCGGATTGTGCAAGCCTTGATGTGGATAATCCAA
GCGCTGTCTGTGACTTTGCCGTAGAGAATTTGATCGATTTCGTGATTGTAGGGCCCGAAGCTCCACTGGCTGCGGGCGTT
GTGGATATGTTGAGGAATGCCGGAATACTGGTATTTGGACCGACCCAGGCAGCAGCGCAGCTGGAAGCTTCCAAGACCTT
CACGAAGGAGGTCTGCGATGCGGCCGGCGCTCCGACCGCCGCCTGGACACGCTTCACCGAAGCCGCACCTGCACGCGACT
ACGTCCGCGAAAAGGGCGCGCCCGTCGTGGTCAAGGCCGACGGGCTGGCGGCGGGCAAAGGCGTGGTCGTCGCCATGACC
GACGGCGACGCATTGGCCGCCATCGACCACATGTTCGACGGCGCGTTCGGGGCCGCCGGAGCGGAGGTGGTGATTGAAGA
ATTCATGGAGGGGGAGGAGGCGTCGTTCTTCGTGCTGTGTGACGGGGACACCTGCCTGCCGATTGGCACCGCCCAGGACC
ACAAGCGCGCCTATGACGGCGACGAAGGCCCCAATACCGGCGGCATGGGGGCCTATTCGCCGGCCCCGGTTCTGACCGAT
GCCATTGCGCAGGCGGCCCTTGATCAGATCATCCGCCCCACCATGGCCGAGATGAAGCGGCGCGGCACGCCCTATACGGG
CGTTCTCTACGCCGGGTTGATGATCCACGACGGCAAGCCCCGGCTGGTGGAATACAACGCCCGTTTCGGCGACCCGGAAT
GTCAGGTCCTGATGATGCGCCTCGGCGGGCAGGCGCTGGACCTGATGCTGGCCTGCGCTGAGGGGCGGTTGGCGGATGCC
CAGGTGAACTGGGCCAATGACCACGCGCTCACGGTCGTCATGGCGGCGCAGGGATATCCCGGTGGATATGAAAAAGGCAG
CGCGATCGCGGGCCTGGATGCCCTGCCCGAGACGTCCTCTCAAATGGTATTCCATGCGGGCACAACGGCCCGGGACGGAC
AGATTACGGCGACCGGGGGCCGGGTGCTGAACGTCACAGCGCGCGGTGCGACCCTGCAAGAGGCGCGCGATCAGGCCTAT
GCGATGGCAAGTCATATCGAGTGGCCGGAGGGCTTTTTCCGCACGGATATCGGCTGGCGGGCGTTATAA

Upstream 100 bases:

>100_bases
GAAGCCGTGTGACTGAAGTCGTGCCCAAAGCTCTGGGTTGCGCATGGTTGTGCCGTCCTCAACTGCTCTTTATGCCTACG
GCCAAGATAGCAGAGGGGAC

Downstream 100 bases:

>100_bases
TAATCGACCTACGGCGCGGCGCGGACCTTGAGGCCGGGTCAGTCCAGACCCAAACGACGGGACAAGCCCCACCCTACAGC
CATGTCCCATAATCCGAGAC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MHLNILILGSGGREHSLAWAVLQNPKCDRLIVAPGNAGIDQIADCASLDVDNPSAVCDFAVENLIDFVIVGPEAPLAAGV
VDMLRNAGILVFGPTQAAAQLEASKTFTKEVCDAAGAPTAAWTRFTEAAPARDYVREKGAPVVVKADGLAAGKGVVVAMT
DGDALAAIDHMFDGAFGAAGAEVVIEEFMEGEEASFFVLCDGDTCLPIGTAQDHKRAYDGDEGPNTGGMGAYSPAPVLTD
AIAQAALDQIIRPTMAEMKRRGTPYTGVLYAGLMIHDGKPRLVEYNARFGDPECQVLMMRLGGQALDLMLACAEGRLADA
QVNWANDHALTVVMAAQGYPGGYEKGSAIAGLDALPETSSQMVFHAGTTARDGQITATGGRVLNVTARGATLQEARDQAY
AMASHIEWPEGFFRTDIGWRAL

Sequences:

>Translated_422_residues
MHLNILILGSGGREHSLAWAVLQNPKCDRLIVAPGNAGIDQIADCASLDVDNPSAVCDFAVENLIDFVIVGPEAPLAAGV
VDMLRNAGILVFGPTQAAAQLEASKTFTKEVCDAAGAPTAAWTRFTEAAPARDYVREKGAPVVVKADGLAAGKGVVVAMT
DGDALAAIDHMFDGAFGAAGAEVVIEEFMEGEEASFFVLCDGDTCLPIGTAQDHKRAYDGDEGPNTGGMGAYSPAPVLTD
AIAQAALDQIIRPTMAEMKRRGTPYTGVLYAGLMIHDGKPRLVEYNARFGDPECQVLMMRLGGQALDLMLACAEGRLADA
QVNWANDHALTVVMAAQGYPGGYEKGSAIAGLDALPETSSQMVFHAGTTARDGQITATGGRVLNVTARGATLQEARDQAY
AMASHIEWPEGFFRTDIGWRAL
>Mature_422_residues
MHLNILILGSGGREHSLAWAVLQNPKCDRLIVAPGNAGIDQIADCASLDVDNPSAVCDFAVENLIDFVIVGPEAPLAAGV
VDMLRNAGILVFGPTQAAAQLEASKTFTKEVCDAAGAPTAAWTRFTEAAPARDYVREKGAPVVVKADGLAAGKGVVVAMT
DGDALAAIDHMFDGAFGAAGAEVVIEEFMEGEEASFFVLCDGDTCLPIGTAQDHKRAYDGDEGPNTGGMGAYSPAPVLTD
AIAQAALDQIIRPTMAEMKRRGTPYTGVLYAGLMIHDGKPRLVEYNARFGDPECQVLMMRLGGQALDLMLACAEGRLADA
QVNWANDHALTVVMAAQGYPGGYEKGSAIAGLDALPETSSQMVFHAGTTARDGQITATGGRVLNVTARGATLQEARDQAY
AMASHIEWPEGFFRTDIGWRAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=430, Percent_Identity=45.8139534883721, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI209869995, Length=430, Percent_Identity=45.8139534883721, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI209869993, Length=430, Percent_Identity=45.8139534883721, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI28416899, Length=430, Percent_Identity=45.8139534883721, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=52.7058823529412, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=42.0427553444181, Blast_Score=308, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=40.7239819004525, Blast_Score=303, Evalue=4e-83,
Organism=Drosophila melanogaster, GI62471619, Length=433, Percent_Identity=40.4157043879908, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24582400, Length=433, Percent_Identity=40.4157043879908, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44272; Mature: 44272

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLNILILGSGGREHSLAWAVLQNPKCDRLIVAPGNAGIDQIADCASLDVDNPSAVCDFA
CEEEEEEECCCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHH
VENLIDFVIVGPEAPLAAGVVDMLRNAGILVFGPTQAAAQLEASKTFTKEVCDAAGAPTA
HHHHEEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHH
AWTRFTEAAPARDYVREKGAPVVVKADGLAAGKGVVVAMTDGDALAAIDHMFDGAFGAAG
HHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCH
AEVVIEEFMEGEEASFFVLCDGDTCLPIGTAQDHKRAYDGDEGPNTGGMGAYSPAPVLTD
HHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
AIAQAALDQIIRPTMAEMKRRGTPYTGVLYAGLMIHDGKPRLVEYNARFGDPECQVLMMR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHH
LGGQALDLMLACAEGRLADAQVNWANDHALTVVMAAQGYPGGYEKGSAIAGLDALPETSS
HCCHHHHHHHHHCCCCEECCEEEECCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCC
QMVFHAGTTARDGQITATGGRVLNVTARGATLQEARDQAYAMASHIEWPEGFFRTDIGWR
CEEEECCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
AL
CC
>Mature Secondary Structure
MHLNILILGSGGREHSLAWAVLQNPKCDRLIVAPGNAGIDQIADCASLDVDNPSAVCDFA
CEEEEEEECCCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHH
VENLIDFVIVGPEAPLAAGVVDMLRNAGILVFGPTQAAAQLEASKTFTKEVCDAAGAPTA
HHHHEEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHH
AWTRFTEAAPARDYVREKGAPVVVKADGLAAGKGVVVAMTDGDALAAIDHMFDGAFGAAG
HHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCH
AEVVIEEFMEGEEASFFVLCDGDTCLPIGTAQDHKRAYDGDEGPNTGGMGAYSPAPVLTD
HHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
AIAQAALDQIIRPTMAEMKRRGTPYTGVLYAGLMIHDGKPRLVEYNARFGDPECQVLMMR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHH
LGGQALDLMLACAEGRLADAQVNWANDHALTVVMAAQGYPGGYEKGSAIAGLDALPETSS
HCCHHHHHHHHHCCCCEECCEEEECCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCC
QMVFHAGTTARDGQITATGGRVLNVTARGATLQEARDQAYAMASHIEWPEGFFRTDIGWR
CEEEECCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]