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The map label for this gene is metG

Identifier: 89055636

GI number: 89055636

Start: 3184792

End: 3186504

Strand: Direct

Name: metG

Synonym: Jann_3145

Alternate gene names: 89055636

Gene position: 3184792-3186504 (Clockwise)

Preceding gene: 89055635

Following gene: 89055647

Centisome position: 73.76

GC content: 61.94

Gene sequence:

>1713_bases
ATGTCCACCACCTCCCGCACGCTTATCACCAGCGCCATTCCCTACATCAACGGGATCAAGCATCTGGGCAATCTCGTGGG
CTCTCAATTACCCGCCGATCTCTACGCCCGTTACTTGCGCGCGCGGGGCCAGGAGGTCTTGTTTCTTTGTGCCACCGACG
AACACGGCACGCCCGCCGAATTAGCGGCGGCCAAGGCCGGCAAGCCCGTGGCTGAATATTGCGCGGACATGCACGCGGTC
CAGGCCGAGATCGCGCGTGGCTTCCGCCTGTCTTTTGATCATTTCGGTCGCTCCTCCTCGCCGCAAAACCATGCGCTGAC
GCAACATTTTGCGGGCAAGCTGGCCGATGCCGGTCTGATCGCTGAGGTCGAGGAAAAGCAGGTCTATTCCCACGCCGACG
GGCGTTTCCTTCCCGACCGCTACATTGAGGGGACCTGCCCCAATTGCGGCTACGACAAGGCCCGTGGCGATCAATGCGAG
AACTGCACCAAGCAGCTTGACCCGACCGATCTGATCAACCCGCGCTCCGCCATCTCAGGCTCCACTGATCTGGAGGTCCG
CGAAACAAAACACCTCTACCTGCGTCAATCGCAGCTGCGCGGACAGCTGAGCGATTGGATTTCGTCCAAAACCGACTGGC
CCATTCTGACCACCTCCATCGCGAAGAAATGGTTAAACGACGGCGACGGATTGCAGGACCGCGGCATCACCCGGGACCTT
GACTGGGGCGTGCCCGTGAAACGCGGCGACGCGGACTGGCCCGGCATGGAAGGGAAGGTCTTCTACGTCTGGTTCGACGC
CCCGATCGAGTATATTGCCTGCGCGGCGGAGGCTGTGGAGGAAGGCAGTATTTCTGATTGGGAACGCTGGTGGCGTGTGG
ATAAGGGCGCGGATGATGTCCGATATGTGCAATTCATGGGGAAGGACAACGTGCCGTTCCACACCCTGTCTTTCCCGGCC
ACGATCCTTGGCTCCGGTGAGCCCTGGAAGCTGGTCGACTACATCAAATCCTTCAACTATCTCAACTATGATGGCGGCCA
GTTCAGCACCTCACAAGGGCGCGGCGTGTTCATGGATCAGGCGCTGGAGATCCTGCCGTCCGATTACTGGCGCTGGTGGC
TTCTGTCCCACGCGCCCGAAACCTCGGACGCGGAATTCACGTGGGAGGCGTTTCAACAGGACGTCAATAAAGACCTCGCC
GATGTGTTGGGGAACTTCGTCAGCCGCATTACCAAATTCTGCCGCTCCAAATTCGGGGAGGTGGTGCCCGAGGGCGGCGA
CTACGGGCCGCAGGAAACGGCCCTGATCGCGGATCTCGACAAGCGCCTTGCCGCCTATCAGACCCACATGGACGCCATCG
ACATCCGCAAGGCCGCCGCTGAGTTGCGCGCCATCTGGGTCGCGGGCAACGAGTATCTGCAATCGGCCGCCCCCTGGACT
GCCTTCAAGACGGACCCGGACCGCGCCGCCGCGATCACTCGCTTCGCCCTGAACCTGATCCCGTTTTACGCGGGCCTCTC
CGCCCCGTTTATCCCCGATGCGGCCCAGGCCATGGCCGAGGGCATGCACACGAACCTGACCTGGCCGAAGGACGCACGCC
TCACCACCCTGCCCGCCGGTCACGCCTTCACGGTGCCCGACGTGCTGTTCGCGAAAATTGCCGATGAGCAGCGCGAAGAC
TGGGCCGCCCGGTTCTCTGGCGTTCGGGATTAG

Upstream 100 bases:

>100_bases
CAACCGGATGTGACACCACGATTGTCTGTGGGGGCACTGGCCTTGCGCGGACCGCGGCGTTAAAGCTGCGCGCAACCGCA
TGCTTTGAAAGCCCGTTTCG

Downstream 100 bases:

>100_bases
ATCTGCACTGACGCACCTTTTGGGCAATTTCGCGCCACAAGGTGCAGCAATCGTGTAGTTACAGAAAAACTACGGAAAAA
CTACAGGTTCTTGCACCCTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSTTSRTLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGQEVLFLCATDEHGTPAELAAAKAGKPVAEYCADMHAV
QAEIARGFRLSFDHFGRSSSPQNHALTQHFAGKLADAGLIAEVEEKQVYSHADGRFLPDRYIEGTCPNCGYDKARGDQCE
NCTKQLDPTDLINPRSAISGSTDLEVRETKHLYLRQSQLRGQLSDWISSKTDWPILTTSIAKKWLNDGDGLQDRGITRDL
DWGVPVKRGDADWPGMEGKVFYVWFDAPIEYIACAAEAVEEGSISDWERWWRVDKGADDVRYVQFMGKDNVPFHTLSFPA
TILGSGEPWKLVDYIKSFNYLNYDGGQFSTSQGRGVFMDQALEILPSDYWRWWLLSHAPETSDAEFTWEAFQQDVNKDLA
DVLGNFVSRITKFCRSKFGEVVPEGGDYGPQETALIADLDKRLAAYQTHMDAIDIRKAAAELRAIWVAGNEYLQSAAPWT
AFKTDPDRAAAITRFALNLIPFYAGLSAPFIPDAAQAMAEGMHTNLTWPKDARLTTLPAGHAFTVPDVLFAKIADEQRED
WAARFSGVRD

Sequences:

>Translated_570_residues
MSTTSRTLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGQEVLFLCATDEHGTPAELAAAKAGKPVAEYCADMHAV
QAEIARGFRLSFDHFGRSSSPQNHALTQHFAGKLADAGLIAEVEEKQVYSHADGRFLPDRYIEGTCPNCGYDKARGDQCE
NCTKQLDPTDLINPRSAISGSTDLEVRETKHLYLRQSQLRGQLSDWISSKTDWPILTTSIAKKWLNDGDGLQDRGITRDL
DWGVPVKRGDADWPGMEGKVFYVWFDAPIEYIACAAEAVEEGSISDWERWWRVDKGADDVRYVQFMGKDNVPFHTLSFPA
TILGSGEPWKLVDYIKSFNYLNYDGGQFSTSQGRGVFMDQALEILPSDYWRWWLLSHAPETSDAEFTWEAFQQDVNKDLA
DVLGNFVSRITKFCRSKFGEVVPEGGDYGPQETALIADLDKRLAAYQTHMDAIDIRKAAAELRAIWVAGNEYLQSAAPWT
AFKTDPDRAAAITRFALNLIPFYAGLSAPFIPDAAQAMAEGMHTNLTWPKDARLTTLPAGHAFTVPDVLFAKIADEQRED
WAARFSGVRD
>Mature_569_residues
STTSRTLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGQEVLFLCATDEHGTPAELAAAKAGKPVAEYCADMHAVQ
AEIARGFRLSFDHFGRSSSPQNHALTQHFAGKLADAGLIAEVEEKQVYSHADGRFLPDRYIEGTCPNCGYDKARGDQCEN
CTKQLDPTDLINPRSAISGSTDLEVRETKHLYLRQSQLRGQLSDWISSKTDWPILTTSIAKKWLNDGDGLQDRGITRDLD
WGVPVKRGDADWPGMEGKVFYVWFDAPIEYIACAAEAVEEGSISDWERWWRVDKGADDVRYVQFMGKDNVPFHTLSFPAT
ILGSGEPWKLVDYIKSFNYLNYDGGQFSTSQGRGVFMDQALEILPSDYWRWWLLSHAPETSDAEFTWEAFQQDVNKDLAD
VLGNFVSRITKFCRSKFGEVVPEGGDYGPQETALIADLDKRLAAYQTHMDAIDIRKAAAELRAIWVAGNEYLQSAAPWTA
FKTDPDRAAAITRFALNLIPFYAGLSAPFIPDAAQAMAEGMHTNLTWPKDARLTTLPAGHAFTVPDVLFAKIADEQREDW
AARFSGVRD

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=37.9130434782609, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI24308436, Length=571, Percent_Identity=24.8686514886165, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1788432, Length=538, Percent_Identity=30.1115241635688, Blast_Score=233, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17540794, Length=578, Percent_Identity=33.9100346020761, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI212645096, Length=545, Percent_Identity=23.8532110091743, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71992781, Length=435, Percent_Identity=22.5287356321839, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321704, Length=570, Percent_Identity=35.4385964912281, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655311, Length=573, Percent_Identity=36.8237347294939, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21358483, Length=539, Percent_Identity=24.860853432282, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_JANSC (Q28MK0)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_511087.1
- ProteinModelPortal:   Q28MK0
- SMR:   Q28MK0
- STRING:   Q28MK0
- GeneID:   3935616
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_3145
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   GSREPWK
- PhylomeDB:   Q28MK0
- ProtClustDB:   PRK12268
- BioCyc:   JSP290400:JANN_3145-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 63562; Mature: 63430

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTSRTLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGQEVLFLCATDEHGTPAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
LAAAKAGKPVAEYCADMHAVQAEIARGFRLSFDHFGRSSSPQNHALTQHFAGKLADAGLI
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCHH
AEVEEKQVYSHADGRFLPDRYIEGTCPNCGYDKARGDQCENCTKQLDPTDLINPRSAISG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCC
STDLEVRETKHLYLRQSQLRGQLSDWISSKTDWPILTTSIAKKWLNDGDGLQDRGITRDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
DWGVPVKRGDADWPGMEGKVFYVWFDAPIEYIACAAEAVEEGSISDWERWWRVDKGADDV
CCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
RYVQFMGKDNVPFHTLSFPATILGSGEPWKLVDYIKSFNYLNYDGGQFSTSQGRGVFMDQ
HHHHEECCCCCCEEEECCCHHEECCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHH
ALEILPSDYWRWWLLSHAPETSDAEFTWEAFQQDVNKDLADVLGNFVSRITKFCRSKFGE
HHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPEGGDYGPQETALIADLDKRLAAYQTHMDAIDIRKAAAELRAIWVAGNEYLQSAAPWT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHCCCCC
AFKTDPDRAAAITRFALNLIPFYAGLSAPFIPDAAQAMAEGMHTNLTWPKDARLTTLPAG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEECCCC
HAFTVPDVLFAKIADEQREDWAARFSGVRD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STTSRTLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGQEVLFLCATDEHGTPAE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
LAAAKAGKPVAEYCADMHAVQAEIARGFRLSFDHFGRSSSPQNHALTQHFAGKLADAGLI
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCHH
AEVEEKQVYSHADGRFLPDRYIEGTCPNCGYDKARGDQCENCTKQLDPTDLINPRSAISG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCC
STDLEVRETKHLYLRQSQLRGQLSDWISSKTDWPILTTSIAKKWLNDGDGLQDRGITRDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
DWGVPVKRGDADWPGMEGKVFYVWFDAPIEYIACAAEAVEEGSISDWERWWRVDKGADDV
CCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
RYVQFMGKDNVPFHTLSFPATILGSGEPWKLVDYIKSFNYLNYDGGQFSTSQGRGVFMDQ
HHHHEECCCCCCEEEECCCHHEECCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHH
ALEILPSDYWRWWLLSHAPETSDAEFTWEAFQQDVNKDLADVLGNFVSRITKFCRSKFGE
HHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPEGGDYGPQETALIADLDKRLAAYQTHMDAIDIRKAAAELRAIWVAGNEYLQSAAPWT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHCCCCC
AFKTDPDRAAAITRFALNLIPFYAGLSAPFIPDAAQAMAEGMHTNLTWPKDARLTTLPAG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEECCCC
HAFTVPDVLFAKIADEQREDWAARFSGVRD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA