Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 89055306

GI number: 89055306

Start: 2829772

End: 2830557

Strand: Direct

Name: xthA [H]

Synonym: Jann_2815

Alternate gene names: 89055306

Gene position: 2829772-2830557 (Clockwise)

Preceding gene: 89055304

Following gene: 89055310

Centisome position: 65.53

GC content: 63.36

Gene sequence:

>786_bases
ATGATGCGGATTGCGACGTTCAATATCAACGGGGTGAAGGCGCGCATCGGCGCGCTCGGGGATTGGCTGGACGAGGCCAA
GCCCGATGTCGCGCTGTTGCAGGAGATCAAATCCGTCGATGAAAATTTCCCGCGAGAGATGTTGGAAGACCGGGGATATA
CCGTGGAAACCCATGGCCAGAAGGGGTTTAATGGGGTCGCGATCCTGTCCAAACTGCCGCTGGAAGATGTCAGCCGTGGG
CTGCCCGGTGATGACACGGATGAACAGGCCCGCTGGATTGAGGCGACGGTGATGGGCGACACGCAGGCGGTGCGGGTCTG
CGGCCTCTATCTGCCCAACGGCAACCCCACGCCGGGCCCGAAATACGATTACAAACTCGCCTGGATGGAGCGGATGCACC
GCCACGCACAGGGCCTTCTCGCCAGCGAAATGCCCGTCGTCATGGCCGGGGATTACAATATCATCCCGCAAGACGCCGAT
GCCGCCCGCCCCGAGGTCTGGCAGGACGACGCCCTCGCCCGCTCCGAAAGCCGCGCCGCCTTCCGCAGGCTCCTCGCGCT
GGGGTTCACCGAGGCGTTTCGCGCCCGCAATCAGGCGCCCGGCATGTATTCCTTCTGGGATTATCAGGCCGGTGCGTGGA
ACAAGAATGACGGCATCCGCATCGACCACCACCTGCTGAGCCCAGAGGCCGCGGACCTCCTGCAGGAATGCTGGATTGAG
AAGGACGTGCGCGGCCGCGACAAACCCTCTGACCACGTCCCCGTCTGGATCGACCTGGCCGCATAA

Upstream 100 bases:

>100_bases
AGCGCAGAAAAGGGCCCGCGCCCAATCTCGGCGACACACCGCCAACTGATCGCTTGGCAAGCCCCCGCCAAAGGGGCAAA
ACAGGGCAAACGGGGGCCAG

Downstream 100 bases:

>100_bases
GACCTGCGGGACGGTGGGAGGGGCGAGGTCGACGTGTTTTGCATCGCAAAATTCGTTGACCAGCGTCTCCCCGTCCCGCC
CCTACAGCCCGTTCAGCGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MMRIATFNINGVKARIGALGDWLDEAKPDVALLQEIKSVDENFPREMLEDRGYTVETHGQKGFNGVAILSKLPLEDVSRG
LPGDDTDEQARWIEATVMGDTQAVRVCGLYLPNGNPTPGPKYDYKLAWMERMHRHAQGLLASEMPVVMAGDYNIIPQDAD
AARPEVWQDDALARSESRAAFRRLLALGFTEAFRARNQAPGMYSFWDYQAGAWNKNDGIRIDHHLLSPEAADLLQECWIE
KDVRGRDKPSDHVPVWIDLAA

Sequences:

>Translated_261_residues
MMRIATFNINGVKARIGALGDWLDEAKPDVALLQEIKSVDENFPREMLEDRGYTVETHGQKGFNGVAILSKLPLEDVSRG
LPGDDTDEQARWIEATVMGDTQAVRVCGLYLPNGNPTPGPKYDYKLAWMERMHRHAQGLLASEMPVVMAGDYNIIPQDAD
AARPEVWQDDALARSESRAAFRRLLALGFTEAFRARNQAPGMYSFWDYQAGAWNKNDGIRIDHHLLSPEAADLLQECWIE
KDVRGRDKPSDHVPVWIDLAA
>Mature_261_residues
MMRIATFNINGVKARIGALGDWLDEAKPDVALLQEIKSVDENFPREMLEDRGYTVETHGQKGFNGVAILSKLPLEDVSRG
LPGDDTDEQARWIEATVMGDTQAVRVCGLYLPNGNPTPGPKYDYKLAWMERMHRHAQGLLASEMPVVMAGDYNIIPQDAD
AARPEVWQDDALARSESRAAFRRLLALGFTEAFRARNQAPGMYSFWDYQAGAWNKNDGIRIDHHLLSPEAADLLQECWIE
KDVRGRDKPSDHVPVWIDLAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=266, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI18375503, Length=266, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI18375501, Length=266, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=36.1216730038023, Blast_Score=148, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71989536, Length=272, Percent_Identity=28.3088235294118, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI221330655, Length=264, Percent_Identity=28.7878787878788, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29289; Mature: 29289

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIATFNINGVKARIGALGDWLDEAKPDVALLQEIKSVDENFPREMLEDRGYTVETHGQ
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEECCC
KGFNGVAILSKLPLEDVSRGLPGDDTDEQARWIEATVMGDTQAVRVCGLYLPNGNPTPGP
CCCCHHHHHHHCCHHHHHCCCCCCCCCHHHHEEEEEECCCCCEEEEEEEECCCCCCCCCC
KYDYKLAWMERMHRHAQGLLASEMPVVMAGDYNIIPQDADAARPEVWQDDALARSESRAA
CCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCCCCCCCCCCHHHHHHHHHHH
FRRLLALGFTEAFRARNQAPGMYSFWDYQAGAWNKNDGIRIDHHLLSPEAADLLQECWIE
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEHEECCCHHHHHHHHHHHC
KDVRGRDKPSDHVPVWIDLAA
CCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MMRIATFNINGVKARIGALGDWLDEAKPDVALLQEIKSVDENFPREMLEDRGYTVETHGQ
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEECCC
KGFNGVAILSKLPLEDVSRGLPGDDTDEQARWIEATVMGDTQAVRVCGLYLPNGNPTPGP
CCCCHHHHHHHCCHHHHHCCCCCCCCCHHHHEEEEEECCCCCEEEEEEEECCCCCCCCCC
KYDYKLAWMERMHRHAQGLLASEMPVVMAGDYNIIPQDADAARPEVWQDDALARSESRAA
CCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCCCCCCCCCCHHHHHHHHHHH
FRRLLALGFTEAFRARNQAPGMYSFWDYQAGAWNKNDGIRIDHHLLSPEAADLLQECWIE
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEHEECCCHHHHHHHHHHHC
KDVRGRDKPSDHVPVWIDLAA
CCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]