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The map label for this gene is gsiB [H]

Identifier: 89055310

GI number: 89055310

Start: 2832541

End: 2834130

Strand: Direct

Name: gsiB [H]

Synonym: Jann_2819

Alternate gene names: 89055310

Gene position: 2832541-2834130 (Clockwise)

Preceding gene: 89055306

Following gene: 89055311

Centisome position: 65.6

GC content: 62.96

Gene sequence:

>1590_bases
ATGACCAACCGACTATTCGCGATCACCGCGACCCTTCTGGCCACCACGGCCATCACCGCCACATCGCTATCGGCCGAGAC
GCTGCGATGGGCGCGCGCCGCCGAGGCGCTGACGCTGGATCCGCATTCCCAGAACGAAGGGCCGACCACGACCCTCATGC
ACCAGATCTACGACCCCCTCATCGTGCGCAACATGGCCGGTGAGATGGAACCCTCGCTCGCCACCGAATGGGCCCCGTCC
GAGGACAACCCCAACGTCTGGGTCTTCCAGATCCGCGAAGGCGTGACCTTCCATGGCGGCGAAAGCTTTGACGCCTCCGA
CGTCGTCTTCTCGCTCAACCGCGCGATGCAGGAGGCCTCGAACTTCAAGGAATTGCTGTCCGGCGTCGTTGAGGTCCGCG
CCACGGGTGATTTCACCGTCGAGATTGAAACCGAAGGCCCCAACCCGCTGCTGGCCAACAACCTGACCAACATCATGATC
ATGGATGAGGGTTGGACCGTCGAAAACGGCGCTGAAAACGTGCAGGATTATGCCGCAGGCGAAGACACGTTCGCCGCGCG
CAACGTCAACGGCACCGGCGCATTCGTGCTGCAAAGCCGGGAGGCTGACGTGCAGACCGTGCTGACCCAGAACCCCGATT
ACTGGGGCATCGGTCAGTTCCCGATGGATGTGACCGAGATCATCTTCACCCCGATCCAGAACCCCGCCACGCGCCTCGCG
GCCTTCCTGGGCGGCGAAGTCGACTTCATCCAGGACGTGCCTGTGCAGGATCTGGAGCGGGTCGAGGCCGCCGACGGCAT
CGGTCTGGGCACCGGACCGCAGAACCGCAACATCTTCTTCGGCCTCAATGTCGGCGCCGATGATCTGGAGCGCGACAACG
TGGACGGGGAAAACCCGCTGGCCAATCCCATGGTCCGGGCCGCGATGAACCTCGCCATCAACCGCGAGGCGATCCAGCAG
GTCGTCATGCGGGGCCAGTCCGATCCCACCGGCGCAATGCTGCCTCCGCCCGTCAACGGCTGGACCGAGGATCTGGAAGC
CTACCCAGCCTATGACGTGGCCGCAGCCCAGGCGCTGATGGATGAGGCAGGCTATGGCGACGGCTTCTCGATCCAGCTCG
ATTGCCCCAATGACCGCTACGTCAACGACGAATCGATCTGCCAGGCGGCCGTGGGCATGTTCGCCCAGATCGGCATCACG
GTGAACCTGTCCGCCCTGCCCCGCGCCCAGCATTTCCCGCTGATCGCCAATGGCGAAACGGACCTGTACATGCTGGGGTG
GGGCGTGCCGACATATGATTCCGAGTATGTCTTCAACTTCCTCTACCACACCCGCAACGAAGGCCGTGGAAGCTGGAACG
GCACCGGCTTCTCCAACGCCGCGATGGACGACATGATCGTGTCCCTGTCGTCGGAGACGGATCTTGAGGCCCGCGATCAG
ATCATCGCCGATCTCTGGACCATGGCCACCGCCGAGCAGATCTACCTGCCCATCCACCACCAGGTCCTCAACTGGGGCAT
CTCCGATGCCTGGAGCACGCTGGTGGACGCCGACGATCAGGTGAAGTTCAAGTATTTTGAACTGAACTGA

Upstream 100 bases:

>100_bases
GCGGTGTCCAAGCCCTGTCTGGACATCTCCCCCGCCGCGCCAATACTCTGCCCCACGGCAACGTCCGCACCCCGACACGG
GGACCAACCGGGAGACTACA

Downstream 100 bases:

>100_bases
CGCCTCAGAACGCAGGCGCGTCACAGCCGTCAAGGCCTGCATGCCCGGGGGACATCTCCCGGGCATGTTGCGGTCTGATG
TCCTGCAAAGACAGGGGCAA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTNRLFAITATLLATTAITATSLSAETLRWARAAEALTLDPHSQNEGPTTTLMHQIYDPLIVRNMAGEMEPSLATEWAPS
EDNPNVWVFQIREGVTFHGGESFDASDVVFSLNRAMQEASNFKELLSGVVEVRATGDFTVEIETEGPNPLLANNLTNIMI
MDEGWTVENGAENVQDYAAGEDTFAARNVNGTGAFVLQSREADVQTVLTQNPDYWGIGQFPMDVTEIIFTPIQNPATRLA
AFLGGEVDFIQDVPVQDLERVEAADGIGLGTGPQNRNIFFGLNVGADDLERDNVDGENPLANPMVRAAMNLAINREAIQQ
VVMRGQSDPTGAMLPPPVNGWTEDLEAYPAYDVAAAQALMDEAGYGDGFSIQLDCPNDRYVNDESICQAAVGMFAQIGIT
VNLSALPRAQHFPLIANGETDLYMLGWGVPTYDSEYVFNFLYHTRNEGRGSWNGTGFSNAAMDDMIVSLSSETDLEARDQ
IIADLWTMATAEQIYLPIHHQVLNWGISDAWSTLVDADDQVKFKYFELN

Sequences:

>Translated_529_residues
MTNRLFAITATLLATTAITATSLSAETLRWARAAEALTLDPHSQNEGPTTTLMHQIYDPLIVRNMAGEMEPSLATEWAPS
EDNPNVWVFQIREGVTFHGGESFDASDVVFSLNRAMQEASNFKELLSGVVEVRATGDFTVEIETEGPNPLLANNLTNIMI
MDEGWTVENGAENVQDYAAGEDTFAARNVNGTGAFVLQSREADVQTVLTQNPDYWGIGQFPMDVTEIIFTPIQNPATRLA
AFLGGEVDFIQDVPVQDLERVEAADGIGLGTGPQNRNIFFGLNVGADDLERDNVDGENPLANPMVRAAMNLAINREAIQQ
VVMRGQSDPTGAMLPPPVNGWTEDLEAYPAYDVAAAQALMDEAGYGDGFSIQLDCPNDRYVNDESICQAAVGMFAQIGIT
VNLSALPRAQHFPLIANGETDLYMLGWGVPTYDSEYVFNFLYHTRNEGRGSWNGTGFSNAAMDDMIVSLSSETDLEARDQ
IIADLWTMATAEQIYLPIHHQVLNWGISDAWSTLVDADDQVKFKYFELN
>Mature_528_residues
TNRLFAITATLLATTAITATSLSAETLRWARAAEALTLDPHSQNEGPTTTLMHQIYDPLIVRNMAGEMEPSLATEWAPSE
DNPNVWVFQIREGVTFHGGESFDASDVVFSLNRAMQEASNFKELLSGVVEVRATGDFTVEIETEGPNPLLANNLTNIMIM
DEGWTVENGAENVQDYAAGEDTFAARNVNGTGAFVLQSREADVQTVLTQNPDYWGIGQFPMDVTEIIFTPIQNPATRLAA
FLGGEVDFIQDVPVQDLERVEAADGIGLGTGPQNRNIFFGLNVGADDLERDNVDGENPLANPMVRAAMNLAINREAIQQV
VMRGQSDPTGAMLPPPVNGWTEDLEAYPAYDVAAAQALMDEAGYGDGFSIQLDCPNDRYVNDESICQAAVGMFAQIGITV
NLSALPRAQHFPLIANGETDLYMLGWGVPTYDSEYVFNFLYHTRNEGRGSWNGTGFSNAAMDDMIVSLSSETDLEARDQI
IADLWTMATAEQIYLPIHHQVLNWGISDAWSTLVDADDQVKFKYFELN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=531, Percent_Identity=22.9755178907721, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789966, Length=502, Percent_Identity=24.7011952191235, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081878, Length=470, Percent_Identity=22.5531914893617, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789397, Length=502, Percent_Identity=22.1115537848606, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787762, Length=481, Percent_Identity=22.8690228690229, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787495, Length=529, Percent_Identity=18.9035916824197, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57948; Mature: 57817

Theoretical pI: Translated: 3.83; Mature: 3.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRLFAITATLLATTAITATSLSAETLRWARAAEALTLDPHSQNEGPTTTLMHQIYDPL
CCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHH
IVRNMAGEMEPSLATEWAPSEDNPNVWVFQIREGVTFHGGESFDASDVVFSLNRAMQEAS
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHH
NFKELLSGVVEVRATGDFTVEIETEGPNPLLANNLTNIMIMDEGWTVENGAENVQDYAAG
HHHHHHHHHHEEEECCCEEEEEECCCCCCEECCCCCEEEEEECCCEECCCHHHHHHHHCC
EDTFAARNVNGTGAFVLQSREADVQTVLTQNPDYWGIGQFPMDVTEIIFTPIQNPATRLA
CCCEEEECCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHH
AFLGGEVDFIQDVPVQDLERVEAADGIGLGTGPQNRNIFFGLNVGADDLERDNVDGENPL
HHHCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
ANPMVRAAMNLAINREAIQQVVMRGQSDPTGAMLPPPVNGWTEDLEAYPAYDVAAAQALM
CCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHH
DEAGYGDGFSIQLDCPNDRYVNDESICQAAVGMFAQIGITVNLSALPRAQHFPLIANGET
HHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCC
DLYMLGWGVPTYDSEYVFNFLYHTRNEGRGSWNGTGFSNAAMDDMIVSLSSETDLEARDQ
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEECCCCCCCHHHHH
IIADLWTMATAEQIYLPIHHQVLNWGISDAWSTLVDADDQVKFKYFELN
HHHHHHHHHHHHHEEEEHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure 
TNRLFAITATLLATTAITATSLSAETLRWARAAEALTLDPHSQNEGPTTTLMHQIYDPL
CCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHH
IVRNMAGEMEPSLATEWAPSEDNPNVWVFQIREGVTFHGGESFDASDVVFSLNRAMQEAS
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHH
NFKELLSGVVEVRATGDFTVEIETEGPNPLLANNLTNIMIMDEGWTVENGAENVQDYAAG
HHHHHHHHHHEEEECCCEEEEEECCCCCCEECCCCCEEEEEECCCEECCCHHHHHHHHCC
EDTFAARNVNGTGAFVLQSREADVQTVLTQNPDYWGIGQFPMDVTEIIFTPIQNPATRLA
CCCEEEECCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHH
AFLGGEVDFIQDVPVQDLERVEAADGIGLGTGPQNRNIFFGLNVGADDLERDNVDGENPL
HHHCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
ANPMVRAAMNLAINREAIQQVVMRGQSDPTGAMLPPPVNGWTEDLEAYPAYDVAAAQALM
CCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHH
DEAGYGDGFSIQLDCPNDRYVNDESICQAAVGMFAQIGITVNLSALPRAQHFPLIANGET
HHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCC
DLYMLGWGVPTYDSEYVFNFLYHTRNEGRGSWNGTGFSNAAMDDMIVSLSSETDLEARDQ
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEECCCCCCCHHHHH
IIADLWTMATAEQIYLPIHHQVLNWGISDAWSTLVDADDQVKFKYFELN
HHHHHHHHHHHHHEEEEHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA