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The map label for this gene is hisH

Identifier: 89055280

GI number: 89055280

Start: 2807236

End: 2807874

Strand: Direct

Name: hisH

Synonym: Jann_2789

Alternate gene names: 89055280

Gene position: 2807236-2807874 (Clockwise)

Preceding gene: 89055279

Following gene: 89055281

Centisome position: 65.01

GC content: 62.75

Gene sequence:

>639_bases
ATGACGACCGTTCTGATCGACTATGACGCGGGCAATCTGCACTCCGCCGAGAAGGCGTTTCAACGCATGGCCCTTGAGGC
GGATGCGGGCGCGCTGATCGTCTCTGCCGATCCCGATGTGGTCGCCAGCGCCGACCGTATCGTCCTGCCCGGTGACGGCG
CCTTTCCCGCCTGCCGCCGTGGCCTTCAAACCATCGACGGGTTGGAGGAGGCGCTGTTGCACGCGGTGGAAACCAAAGGA
AAACCGTTTCTGGGCATTTGCGTTGGCATGCAAATGATGACCAGCTGGGGCCGCGAATATGAAGATGTGGCCGGTTTCGG
CTGGATCCCGGGCGAGGTCACGCAGATCGCGCCCTGCGACCCCGCCCTGAAGGTGCCGCATATGGGCTGGAACGATCTGG
TCATCGACCACCCCCACGCGATCCTTGACGGCGTCACCACCGGCGATCACGCCTATTTCGTCCATTCCTATGCCATGCAA
CCCGCAGATCCCGCCGATCTTCTGGCGCATGTGGAGTATGGCGGGCAGGTCACGGCCATCGTCGGGCGCGACACGATGGT
GGGGATGCAGTTCCACCCGGAAAAGAGCCAATCAGCCGGTTTGCGCATGATTGCGAATTTCCTCGCCTGGCGGCCGTAG

Upstream 100 bases:

>100_bases
GTTGGAGGTTGACCCACGCACGGCCGACGCCATCCCATCGACCAAGGGCAGCCTGTAAACGCGCCCTCCAGAGCACATCT
TTGTTTAAGGAACGCACGCG

Downstream 100 bases:

>100_bases
TCGCCGTGCACGCGCAGGGGCGTGCGCCCGCGGTTGAGGCGGACTGCGTCCAATCGTCCGCAAATCCATGTTTGGCCTGT
CCGGCCCACCGCCAACGCGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTTVLIDYDAGNLHSAEKAFQRMALEADAGALIVSADPDVVASADRIVLPGDGAFPACRRGLQTIDGLEEALLHAVETKG
KPFLGICVGMQMMTSWGREYEDVAGFGWIPGEVTQIAPCDPALKVPHMGWNDLVIDHPHAILDGVTTGDHAYFVHSYAMQ
PADPADLLAHVEYGGQVTAIVGRDTMVGMQFHPEKSQSAGLRMIANFLAWRP

Sequences:

>Translated_212_residues
MTTVLIDYDAGNLHSAEKAFQRMALEADAGALIVSADPDVVASADRIVLPGDGAFPACRRGLQTIDGLEEALLHAVETKG
KPFLGICVGMQMMTSWGREYEDVAGFGWIPGEVTQIAPCDPALKVPHMGWNDLVIDHPHAILDGVTTGDHAYFVHSYAMQ
PADPADLLAHVEYGGQVTAIVGRDTMVGMQFHPEKSQSAGLRMIANFLAWRP
>Mature_211_residues
TTVLIDYDAGNLHSAEKAFQRMALEADAGALIVSADPDVVASADRIVLPGDGAFPACRRGLQTIDGLEEALLHAVETKGK
PFLGICVGMQMMTSWGREYEDVAGFGWIPGEVTQIAPCDPALKVPHMGWNDLVIDHPHAILDGVTTGDHAYFVHSYAMQP
ADPADLLAHVEYGGQVTAIVGRDTMVGMQFHPEKSQSAGLRMIANFLAWRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=37.0192307692308, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22824; Mature: 22693

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVLIDYDAGNLHSAEKAFQRMALEADAGALIVSADPDVVASADRIVLPGDGAFPACRR
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCCEEEECCCCCCHHHHH
GLQTIDGLEEALLHAVETKGKPFLGICVGMQMMTSWGREYEDVAGFGWIPGEVTQIAPCD
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEECCCC
PALKVPHMGWNDLVIDHPHAILDGVTTGDHAYFVHSYAMQPADPADLLAHVEYGGQVTAI
CCCCCCCCCCCCEEEECCHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEE
VGRDTMVGMQFHPEKSQSAGLRMIANFLAWRP
ECCCEEEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTVLIDYDAGNLHSAEKAFQRMALEADAGALIVSADPDVVASADRIVLPGDGAFPACRR
EEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCCEEEECCCCCCHHHHH
GLQTIDGLEEALLHAVETKGKPFLGICVGMQMMTSWGREYEDVAGFGWIPGEVTQIAPCD
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEECCCC
PALKVPHMGWNDLVIDHPHAILDGVTTGDHAYFVHSYAMQPADPADLLAHVEYGGQVTAI
CCCCCCCCCCCCEEEECCHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEE
VGRDTMVGMQFHPEKSQSAGLRMIANFLAWRP
ECCCEEEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA