Click here to switch to the map view.

The map label for this gene is psdht [H]

Identifier: 89055094

GI number: 89055094

Start: 2618596

End: 2619558

Strand: Direct

Name: psdht [H]

Synonym: Jann_2603

Alternate gene names: 89055094

Gene position: 2618596-2619558 (Clockwise)

Preceding gene: 89055093

Following gene: 89055095

Centisome position: 60.64

GC content: 60.85

Gene sequence:

>963_bases
ATGAAAGATACCCTAGATCTTCCAACCTACGACGACATGCTCGCCGCGCATGAGCGGATCAAGCCGCATATCAACCGCAC
GCCGGTGCGCACCTCTGCCTACCTCAATGAACTGACGGGCGCGGAGTTGTTCTTCAAATGCGAGAACTTCCAGGAGCCGG
GCGCCTTCAAAGTGCGTGGCGCGGCCAATGCCGTTTTCGGGTTAAGCGATGCGCAGGCCGAAAAGGGCGTCGCGACACAT
TCGTCGGGCAACCACGCGTCCTGCCTCTCCTATGCGGCGATGCGCCGGGGCATTCCGTGCAACGTTGTGATGCCGCGTAC
CGCGCCGCAGGCCAAGAAAGATACCGTGAAGCGCTATGGCGGGGTGATCACGGAGTGCGACCCGTCCACCACCTCGCGCG
AAGAGACGTTCGCCAAGGTGCAAGCCGCAACAGGCGGCGACTTCGTCCACCCCTATAACGACCCGCGCGTGATCGCGGGC
CAAGGCACCTGCTCCAAGGAATTCATGGAGCAAGTGGACGGGCTCGACATGATGGTTGCCCCCATCGGCGGCGGCGGCAT
GATCTCTGGCACCTGCCTGACCCTTTCAACCCTGGCGCCAGAGGTGCAGATCATCGCGGCAGAGCCGGAACAGGCCGATG
ACGCTTACCGCAGCTTCAAAGCGGGCTATATTATTGCCGATGATGCGCCCAAAACCATCGCCGACGGCCTGCTGGTGCCC
CTGAAAGAACGGACCTGGCACTTCGTCAGCAACCATGTGACCGATATCCTCACCGCATCGGAAGAGGAAATCATCGACGC
GATGAAGATCACCTGGAAGCATCTGCGCATCGTAATGGAGGCCAGCTGCGCCGTGCCGCTCGCCACCATTCTGAAGAACA
AGGATCGCTTCGCGGGCAAACGCGTGGGCGTGATCATTACGGGCGGCAATGTCGATCTCGACAAGCTGCCTTGGACCCAA
TGA

Upstream 100 bases:

>100_bases
TCGACATCAAACTGGGCTCTGGCGTGGCGGCGGCACAGGAATACTATCGCACAAACCCTGCACAATCGGCACTGCCGGTG
GATTGAAGAGAGGTGTGACC

Downstream 100 bases:

>100_bases
ACGACACTTTGAAGGGAGTTCAGAGATGAACAAGCAAGTGAATTTCGATGAGCTGGAAGTTGGTTATGACGTCCCCGCGC
TGCCCGGCATGGATGAGGCT

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: @OXOB02.txt*Oxobutanoate!; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKDTLDLPTYDDMLAAHERIKPHINRTPVRTSAYLNELTGAELFFKCENFQEPGAFKVRGAANAVFGLSDAQAEKGVATH
SSGNHASCLSYAAMRRGIPCNVVMPRTAPQAKKDTVKRYGGVITECDPSTTSREETFAKVQAATGGDFVHPYNDPRVIAG
QGTCSKEFMEQVDGLDMMVAPIGGGGMISGTCLTLSTLAPEVQIIAAEPEQADDAYRSFKAGYIIADDAPKTIADGLLVP
LKERTWHFVSNHVTDILTASEEEIIDAMKITWKHLRIVMEASCAVPLATILKNKDRFAGKRVGVIITGGNVDLDKLPWTQ

Sequences:

>Translated_320_residues
MKDTLDLPTYDDMLAAHERIKPHINRTPVRTSAYLNELTGAELFFKCENFQEPGAFKVRGAANAVFGLSDAQAEKGVATH
SSGNHASCLSYAAMRRGIPCNVVMPRTAPQAKKDTVKRYGGVITECDPSTTSREETFAKVQAATGGDFVHPYNDPRVIAG
QGTCSKEFMEQVDGLDMMVAPIGGGGMISGTCLTLSTLAPEVQIIAAEPEQADDAYRSFKAGYIIADDAPKTIADGLLVP
LKERTWHFVSNHVTDILTASEEEIIDAMKITWKHLRIVMEASCAVPLATILKNKDRFAGKRVGVIITGGNVDLDKLPWTQ
>Mature_320_residues
MKDTLDLPTYDDMLAAHERIKPHINRTPVRTSAYLNELTGAELFFKCENFQEPGAFKVRGAANAVFGLSDAQAEKGVATH
SSGNHASCLSYAAMRRGIPCNVVMPRTAPQAKKDTVKRYGGVITECDPSTTSREETFAKVQAATGGDFVHPYNDPRVIAG
QGTCSKEFMEQVDGLDMMVAPIGGGGMISGTCLTLSTLAPEVQIIAAEPEQADDAYRSFKAGYIIADDAPKTIADGLLVP
LKERTWHFVSNHVTDILTASEEEIIDAMKITWKHLRIVMEASCAVPLATILKNKDRFAGKRVGVIITGGNVDLDKLPWTQ

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=320, Percent_Identity=42.1875, Blast_Score=258, Evalue=4e-69,
Organism=Escherichia coli, GI1789505, Length=329, Percent_Identity=36.1702127659575, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790207, Length=298, Percent_Identity=29.8657718120805, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17508781, Length=313, Percent_Identity=32.9073482428115, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71991565, Length=305, Percent_Identity=32.4590163934426, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17537387, Length=305, Percent_Identity=30.1639344262295, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322631, Length=310, Percent_Identity=42.258064516129, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6320930, Length=307, Percent_Identity=28.9902280130293, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319788, Length=285, Percent_Identity=22.1052631578947, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355833, Length=312, Percent_Identity=32.6923076923077, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645328, Length=217, Percent_Identity=30.8755760368664, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 34702; Mature: 34702

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTLDLPTYDDMLAAHERIKPHINRTPVRTSAYLNELTGAELFFKCENFQEPGAFKVRG
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEC
AANAVFGLSDAQAEKGVATHSSGNHASCLSYAAMRRGIPCNVVMPRTAPQAKKDTVKRYG
CCCEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHC
GVITECDPSTTSREETFAKVQAATGGDFVHPYNDPRVIAGQGTCSKEFMEQVDGLDMMVA
CEEEECCCCCCCHHHHHHHEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCEEEEE
PIGGGGMISGTCLTLSTLAPEVQIIAAEPEQADDAYRSFKAGYIIADDAPKTIADGLLVP
ECCCCCEECCCEEEHHHCCCCEEEEEECCCHHHHHHHHHHCCEEEECCCCHHHHCCCEEE
LKERTWHFVSNHVTDILTASEEEIIDAMKITWKHLRIVMEASCAVPLATILKNKDRFAGK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHCCC
RVGVIITGGNVDLDKLPWTQ
EEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKDTLDLPTYDDMLAAHERIKPHINRTPVRTSAYLNELTGAELFFKCENFQEPGAFKVRG
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEC
AANAVFGLSDAQAEKGVATHSSGNHASCLSYAAMRRGIPCNVVMPRTAPQAKKDTVKRYG
CCCEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHC
GVITECDPSTTSREETFAKVQAATGGDFVHPYNDPRVIAGQGTCSKEFMEQVDGLDMMVA
CEEEECCCCCCCHHHHHHHEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCEEEEE
PIGGGGMISGTCLTLSTLAPEVQIIAAEPEQADDAYRSFKAGYIIADDAPKTIADGLLVP
ECCCCCEECCCEEEHHHCCCCEEEEEECCCHHHHHHHHHHCCEEEECCCCHHHHCCCEEE
LKERTWHFVSNHVTDILTASEEEIIDAMKITWKHLRIVMEASCAVPLATILKNKDRFAGK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHCCC
RVGVIITGGNVDLDKLPWTQ
EEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = @OXOB02.txt*oxobutanoate! + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]