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The map label for this gene is nifS [H]

Identifier: 89054856

GI number: 89054856

Start: 2370240

End: 2371292

Strand: Reverse

Name: nifS [H]

Synonym: Jann_2365

Alternate gene names: 89054856

Gene position: 2371292-2370240 (Counterclockwise)

Preceding gene: 89054857

Following gene: 89054855

Centisome position: 54.92

GC content: 65.34

Gene sequence:

>1053_bases
GTGAGGGACCGTGTCTACCTCGATTGGAACGCAACGGCACCGCTTCGCCCCGTGGCGCGGGCTGCCATGGAGGCGGCCAT
GGATGTGGTTGGAAACCCGTCGTCCGTCCATGCGGAGGGGCGCGCCGCCAAGGCGTTGATGGAGCGCGCGCGCACACAGG
TGGCGGAGGCCTTGGGAGCGCAGGCCGCCGACGTGGTCTTTGTGTCCGGCGCGACGGAAGCGGCGGCTTTGGCGATGGCG
GGCCGGGGTTTGCAGACCTCTGCCATTGAGCATGACGCCGTGCGGGCCTGGGGCCATGAGGACTTGCGCGTGACGCCGGA
GGGGCACGTTGAGATCACGGATCCGTCCCGGGCCGCCGTGCAGATTGCCAATTCCGAGACCGGGATCTTGCAGCATGTGC
CGGACGGCCTGGCCGTGAGCGATTGGACGCAGGGGTTTGGCAAAATCCCCCTGGCGTTCGATTGGTCCGGCATCGACATG
GCATTTGTATCGTCGCACAAGATCGGCGGGCCCAAGGGCGTGGGTGCGCTGATCCTGCGGCGCGGCCTTGATGTTCAAGC
GCAGATCAGGGGCGGCGGGCAGGAAATGGGCCGCCGTCATGGCACGGAGAACCTGATTGGCATTGCTGGATTTGGGGCCG
CAGCGCAGGCCGCGACACAGGATTTGGCGGATGGCTTGTGGGAGCCGGTGGACAAACTTAGAATATTTCTAGAAAAGACT
CTGGAAGACGCCGCAAGCTCGACTATTTTAGTAGGGAAAGCGGTGAGACGCCTCCCCAACACCTCGATGATCATCACCGA
GGGATGGCGGGGCGAGACGCAGGTGATGGCGATGGATCTGGCGGGGTTTGCGGTATCTGCGGGCTCGGCGTGCTCCAGCG
GCAAGGTGAAGATGTCGGGCGTGTTGCGCGCGATGGGATATTCCGAGGCCCAGGCGTCAAGCGCGTTGCGCGTGTCGTTG
GGGCCGTCAGTGACCGAGCGGCAGGTGGCCGCGTTCTGCGAGGCCTGGCTGAGGGCGCGGGACAAGAAGATGGCGCGCGG
TGAGCACGCATAG

Upstream 100 bases:

>100_bases
CGCGCGCGGTGTTACAGGTGGGGTCTGTCGGTGCCTCCGGCGGGGATATTTTAGGAACGGGGAACGGGGGGCGCGGCGCT
GATCGTGCGACCGGTGCGCC

Downstream 100 bases:

>100_bases
AAATTTCCGCAAATGATTGCGGTTTGGAGGGAACGATGAGCGCACTCGATACAGATGTTCAGGTGAAAGACGGTGTCGAT
CAGGAAACGGTGGATGCCGT

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRDRVYLDWNATAPLRPVARAAMEAAMDVVGNPSSVHAEGRAAKALMERARTQVAEALGAQAADVVFVSGATEAAALAMA
GRGLQTSAIEHDAVRAWGHEDLRVTPEGHVEITDPSRAAVQIANSETGILQHVPDGLAVSDWTQGFGKIPLAFDWSGIDM
AFVSSHKIGGPKGVGALILRRGLDVQAQIRGGGQEMGRRHGTENLIGIAGFGAAAQAATQDLADGLWEPVDKLRIFLEKT
LEDAASSTILVGKAVRRLPNTSMIITEGWRGETQVMAMDLAGFAVSAGSACSSGKVKMSGVLRAMGYSEAQASSALRVSL
GPSVTERQVAAFCEAWLRARDKKMARGEHA

Sequences:

>Translated_350_residues
MRDRVYLDWNATAPLRPVARAAMEAAMDVVGNPSSVHAEGRAAKALMERARTQVAEALGAQAADVVFVSGATEAAALAMA
GRGLQTSAIEHDAVRAWGHEDLRVTPEGHVEITDPSRAAVQIANSETGILQHVPDGLAVSDWTQGFGKIPLAFDWSGIDM
AFVSSHKIGGPKGVGALILRRGLDVQAQIRGGGQEMGRRHGTENLIGIAGFGAAAQAATQDLADGLWEPVDKLRIFLEKT
LEDAASSTILVGKAVRRLPNTSMIITEGWRGETQVMAMDLAGFAVSAGSACSSGKVKMSGVLRAMGYSEAQASSALRVSL
GPSVTERQVAAFCEAWLRARDKKMARGEHA
>Mature_350_residues
MRDRVYLDWNATAPLRPVARAAMEAAMDVVGNPSSVHAEGRAAKALMERARTQVAEALGAQAADVVFVSGATEAAALAMA
GRGLQTSAIEHDAVRAWGHEDLRVTPEGHVEITDPSRAAVQIANSETGILQHVPDGLAVSDWTQGFGKIPLAFDWSGIDM
AFVSSHKIGGPKGVGALILRRGLDVQAQIRGGGQEMGRRHGTENLIGIAGFGAAAQAATQDLADGLWEPVDKLRIFLEKT
LEDAASSTILVGKAVRRLPNTSMIITEGWRGETQVMAMDLAGFAVSAGSACSSGKVKMSGVLRAMGYSEAQASSALRVSL
GPSVTERQVAAFCEAWLRARDKKMARGEHA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=373, Percent_Identity=33.5120643431635, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI156713448, Length=400, Percent_Identity=31, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI48994898, Length=375, Percent_Identity=33.8666666666667, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25143064, Length=378, Percent_Identity=31.7460317460317, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17533177, Length=228, Percent_Identity=30.7017543859649, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319831, Length=370, Percent_Identity=31.6216216216216, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI20129463, Length=366, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 36861; Mature: 36861

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00606 B_KETOACYL_SYNTHASE ; PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRVYLDWNATAPLRPVARAAMEAAMDVVGNPSSVHAEGRAAKALMERARTQVAEALGA
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QAADVVFVSGATEAAALAMAGRGLQTSAIEHDAVRAWGHEDLRVTPEGHVEITDPSRAAV
CCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCCCCEEEECCCCEEE
QIANSETGILQHVPDGLAVSDWTQGFGKIPLAFDWSGIDMAFVSSHKIGGPKGVGALILR
EECCCCCCHHHHCCCCCEECHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHH
RGLDVQAQIRGGGQEMGRRHGTENLIGIAGFGAAAQAATQDLADGLWEPVDKLRIFLEKT
CCCCEEEEECCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDAASSTILVGKAVRRLPNTSMIITEGWRGETQVMAMDLAGFAVSAGSACSSGKVKMSG
HHHHHCCEEHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHEECCCCCCCCCEEHHH
VLRAMGYSEAQASSALRVSLGPSVTERQVAAFCEAWLRARDKKMARGEHA
HHHHHCCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDRVYLDWNATAPLRPVARAAMEAAMDVVGNPSSVHAEGRAAKALMERARTQVAEALGA
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QAADVVFVSGATEAAALAMAGRGLQTSAIEHDAVRAWGHEDLRVTPEGHVEITDPSRAAV
CCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCCCCEEEECCCCEEE
QIANSETGILQHVPDGLAVSDWTQGFGKIPLAFDWSGIDMAFVSSHKIGGPKGVGALILR
EECCCCCCHHHHCCCCCEECHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHH
RGLDVQAQIRGGGQEMGRRHGTENLIGIAGFGAAAQAATQDLADGLWEPVDKLRIFLEKT
CCCCEEEEECCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDAASSTILVGKAVRRLPNTSMIITEGWRGETQVMAMDLAGFAVSAGSACSSGKVKMSG
HHHHHCCEEHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHEECCCCCCCCCEEHHH
VLRAMGYSEAQASSALRVSLGPSVTERQVAAFCEAWLRARDKKMARGEHA
HHHHHCCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA