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The map label for this gene is phaZ1 [H]

Identifier: 89054695

GI number: 89054695

Start: 2213128

End: 2214231

Strand: Direct

Name: phaZ1 [H]

Synonym: Jann_2204

Alternate gene names: 89054695

Gene position: 2213128-2214231 (Clockwise)

Preceding gene: 89054694

Following gene: 89054698

Centisome position: 51.25

GC content: 60.87

Gene sequence:

>1104_bases
ATGAAGCTCTTTAACGTAGGCGCGATGCGCCCTGCGACCGATGAGGCGCGTCTGTCGCGCGCCAATGAACTGGTACAGCG
AACGCTGGCGCAACATAATCTTTCTGCCGATTCAACGGGCACTGGTTCGGGCAGGTTGCCTAAGATGGACCCCATATTGT
CCCGTCTAACTAAGGCGGGACATCAGACAACATTCGAACAATCGACCTTGCCGGACGGGGCGCAATTCTTGAGCAACACG
TTCACATGCCGATCTGGCAGCCGCGCGTACCGGATGTATGTGCCCACGTCAGCCTCACGCGGCGTGTCCGGTGTCGTGAT
GATGCTGCACGGGTGTACCCAGACCCCCGAAGACTTTGCGGCGGGAACCGGTATGAACGCTCTGGCCGAGGATTATGGCT
TGATCGTCATCTATCCTGCGCAGTCACGGGGCGACAACGCGCAATCGTGTTGGAACTGGTTCAGTCGTGGCGATCAGGGT
CGGGGCCGCGGTGAGCCCTCTATTCTCGCGGAACTGGCGATGGAGGTGTGCGCGGACCATGGAATTTCGCGTGATCGCAC
CTTCGTCGCGGGTCTGTCTGCGGGCGGCGCGATGGCGGCCATTCTGGGTGAGACTTACCCAGATGTCTTCGCGGCCATTG
GCGTTCATTCCGGACTACCAGTGGGTGCGGCGAAGGATGTCCCCTCGGCATTTGCCGCCATGGGCGGAACGATCCTGGAT
GCGCCTGTTGCCAACCTCGGCAATACACCGATGCGAACCATCGTATTTCACGGCTCGGCAGATAGCACCGTGCATCCAAA
AAATGGCGATCACGTCGCGCGGCGGGCTTTGGATGCTGGTGCGGGCCAGATACTGCTGACATCTCAGCGTGGGGAGAAAA
ATGGCCGAAGCTATACCCAGAGCATTTACAGTGACCCGGCGGGCGCTGCTGTCGTGGAGCACTGGTGCGTAGACGGGCAG
CGCCATGCCTGGTCTGGCGGTCAGGCGGTGGGATCCTACACCGATGCGTCAGGCCCGGACGCCAGCGCGGAAATGGTCCG
CTTCTTTCTTAAGGCTGCGGCAGATACCGCCGTGCACGACAGCCAAACGCCCCGGCGGGATTAA

Upstream 100 bases:

>100_bases
GATCACCGTTTTGGGCGCTTTGCAGGCCAATAAAGATGTCAAGACGGCGCTCGCCGACCGCATTAAGTAAGATGCGCAAC
CCTTTATAAGGAATAGATAA

Downstream 100 bases:

>100_bases
GATCCGGCGATGGACACCGACACGGTCTGCGTGTCCATCGTCATCGGTCCAAAACTACTCAAGAACGCGACTTCTGCGGC
GTCCATCCCAACGCCGATCC

Product: esterase, PHB depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKLFNVGAMRPATDEARLSRANELVQRTLAQHNLSADSTGTGSGRLPKMDPILSRLTKAGHQTTFEQSTLPDGAQFLSNT
FTCRSGSRAYRMYVPTSASRGVSGVVMMLHGCTQTPEDFAAGTGMNALAEDYGLIVIYPAQSRGDNAQSCWNWFSRGDQG
RGRGEPSILAELAMEVCADHGISRDRTFVAGLSAGGAMAAILGETYPDVFAAIGVHSGLPVGAAKDVPSAFAAMGGTILD
APVANLGNTPMRTIVFHGSADSTVHPKNGDHVARRALDAGAGQILLTSQRGEKNGRSYTQSIYSDPAGAAVVEHWCVDGQ
RHAWSGGQAVGSYTDASGPDASAEMVRFFLKAAADTAVHDSQTPRRD

Sequences:

>Translated_367_residues
MKLFNVGAMRPATDEARLSRANELVQRTLAQHNLSADSTGTGSGRLPKMDPILSRLTKAGHQTTFEQSTLPDGAQFLSNT
FTCRSGSRAYRMYVPTSASRGVSGVVMMLHGCTQTPEDFAAGTGMNALAEDYGLIVIYPAQSRGDNAQSCWNWFSRGDQG
RGRGEPSILAELAMEVCADHGISRDRTFVAGLSAGGAMAAILGETYPDVFAAIGVHSGLPVGAAKDVPSAFAAMGGTILD
APVANLGNTPMRTIVFHGSADSTVHPKNGDHVARRALDAGAGQILLTSQRGEKNGRSYTQSIYSDPAGAAVVEHWCVDGQ
RHAWSGGQAVGSYTDASGPDASAEMVRFFLKAAADTAVHDSQTPRRD
>Mature_367_residues
MKLFNVGAMRPATDEARLSRANELVQRTLAQHNLSADSTGTGSGRLPKMDPILSRLTKAGHQTTFEQSTLPDGAQFLSNT
FTCRSGSRAYRMYVPTSASRGVSGVVMMLHGCTQTPEDFAAGTGMNALAEDYGLIVIYPAQSRGDNAQSCWNWFSRGDQG
RGRGEPSILAELAMEVCADHGISRDRTFVAGLSAGGAMAAILGETYPDVFAAIGVHSGLPVGAAKDVPSAFAAMGGTILD
APVANLGNTPMRTIVFHGSADSTVHPKNGDHVARRALDAGAGQILLTSQRGEKNGRSYTQSIYSDPAGAAVVEHWCVDGQ
RHAWSGGQAVGSYTDASGPDASAEMVRFFLKAAADTAVHDSQTPRRD

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38620; Mature: 38620

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFNVGAMRPATDEARLSRANELVQRTLAQHNLSADSTGTGSGRLPKMDPILSRLTKAG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHC
HQTTFEQSTLPDGAQFLSNTFTCRSGSRAYRMYVPTSASRGVSGVVMMLHGCTQTPEDFA
CCCCCHHCCCCCHHHHHHCCCEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHH
AGTGMNALAEDYGLIVIYPAQSRGDNAQSCWNWFSRGDQGRGRGEPSILAELAMEVCADH
CCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
GISRDRTFVAGLSAGGAMAAILGETYPDVFAAIGVHSGLPVGAAKDVPSAFAAMGGTILD
CCCCCCEEEEECCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
APVANLGNTPMRTIVFHGSADSTVHPKNGDHVARRALDAGAGQILLTSQRGEKNGRSYTQ
CCHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
SIYSDPAGAAVVEHWCVDGQRHAWSGGQAVGSYTDASGPDASAEMVRFFLKAAADTAVHD
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
SQTPRRD
CCCCCCC
>Mature Secondary Structure
MKLFNVGAMRPATDEARLSRANELVQRTLAQHNLSADSTGTGSGRLPKMDPILSRLTKAG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHC
HQTTFEQSTLPDGAQFLSNTFTCRSGSRAYRMYVPTSASRGVSGVVMMLHGCTQTPEDFA
CCCCCHHCCCCCHHHHHHCCCEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHH
AGTGMNALAEDYGLIVIYPAQSRGDNAQSCWNWFSRGDQGRGRGEPSILAELAMEVCADH
CCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
GISRDRTFVAGLSAGGAMAAILGETYPDVFAAIGVHSGLPVGAAKDVPSAFAAMGGTILD
CCCCCCEEEEECCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
APVANLGNTPMRTIVFHGSADSTVHPKNGDHVARRALDAGAGQILLTSQRGEKNGRSYTQ
CCHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
SIYSDPAGAAVVEHWCVDGQRHAWSGGQAVGSYTDASGPDASAEMVRFFLKAAADTAVHD
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
SQTPRRD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]