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The map label for this gene is purN [H]

Identifier: 89054328

GI number: 89054328

Start: 1823148

End: 1823741

Strand: Direct

Name: purN [H]

Synonym: Jann_1837

Alternate gene names: 89054328

Gene position: 1823148-1823741 (Clockwise)

Preceding gene: 89054327

Following gene: 89054329

Centisome position: 42.22

GC content: 64.81

Gene sequence:

>594_bases
ATGAGCTTGCGGGTCGCGATCCTGATCTCCGGCGGCGGGTCCAACATGGTCGCGCTGGCCCGGGACATGGTGGGCCATCA
CCCCGCGCGGCCCTGTCTGGTCGTCTCAAACGTTCCGGGGGCCGGAGGATTGGCGAAGGCCGAGACCATGGGTATTCCGA
CAGCCTGCGTGGATCATCGCGCGTTCAAAGGCGACCGCGCAGCGTTTGAGGCCGCTTTGCAGAAGGTGCTGATAGCCCAC
ACCCCCGGTATCCTGTGCCTCGCGGGGTTCATGCGCATCCTGACGCCGGACTTCGTCGCGGGGTGGGAGGGGCAGATGCT
GAACATCCATCCTTCCCTTTTGCCGCTCTACAAAGGTCTCAACACCCATGCCCGCGCGATTGAAGCCGGCGATGCCGAGG
CCGGATGCACGGTCCATGAGGTCACGGCGGCGCTGGATGACGGACCAATCCTCGGTCAGGCCCGCGTGCCGATCCAGTCC
GATGACACGCCTGAGGCCCTTGCCGCGCGCATTTTGCCGCTGGAACACCGCCTCTACCCTGCCGTTTTGCGGCGTTTTGC
CAGCGGAGACCGGACCAAGTTGAAGCTCAGCTAA

Upstream 100 bases:

>100_bases
TCGATGGCCTGACCTGGACATTGGAAGACGCGGGCGAAAGTGTGCACCGCATCGGCACCGTCACGGCAGGCGCGGGCGTG
CGCTACTCGGGATCGCTTGG

Downstream 100 bases:

>100_bases
CCGGCTCAATTCTCCGCAGCGTCTTCCCTTCCGCCTCCGGGTCCTCTACACCCGCAAACAACCTATTTTTCTGACGAAGA
CGCTCCCTCCCGTGCCGCAC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSLRVAILISGGGSNMVALARDMVGHHPARPCLVVSNVPGAGGLAKAETMGIPTACVDHRAFKGDRAAFEAALQKVLIAH
TPGILCLAGFMRILTPDFVAGWEGQMLNIHPSLLPLYKGLNTHARAIEAGDAEAGCTVHEVTAALDDGPILGQARVPIQS
DDTPEALAARILPLEHRLYPAVLRRFASGDRTKLKLS

Sequences:

>Translated_197_residues
MSLRVAILISGGGSNMVALARDMVGHHPARPCLVVSNVPGAGGLAKAETMGIPTACVDHRAFKGDRAAFEAALQKVLIAH
TPGILCLAGFMRILTPDFVAGWEGQMLNIHPSLLPLYKGLNTHARAIEAGDAEAGCTVHEVTAALDDGPILGQARVPIQS
DDTPEALAARILPLEHRLYPAVLRRFASGDRTKLKLS
>Mature_196_residues
SLRVAILISGGGSNMVALARDMVGHHPARPCLVVSNVPGAGGLAKAETMGIPTACVDHRAFKGDRAAFEAALQKVLIAHT
PGILCLAGFMRILTPDFVAGWEGQMLNIHPSLLPLYKGLNTHARAIEAGDAEAGCTVHEVTAALDDGPILGQARVPIQSD
DTPEALAARILPLEHRLYPAVLRRFASGDRTKLKLS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=46.524064171123, Blast_Score=161, Evalue=3e-40,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=46.524064171123, Blast_Score=161, Evalue=3e-40,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=46.524064171123, Blast_Score=161, Evalue=3e-40,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=42.3280423280423, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1787483, Length=152, Percent_Identity=32.8947368421053, Blast_Score=77, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=40.7608695652174, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=44.973544973545, Blast_Score=153, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20779; Mature: 20648

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRVAILISGGGSNMVALARDMVGHHPARPCLVVSNVPGAGGLAKAETMGIPTACVDHR
CCEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHCCH
AFKGDRAAFEAALQKVLIAHTPGILCLAGFMRILTPDFVAGWEGQMLNIHPSLLPLYKGL
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHCCCCCCEEEECHHHHHHHHCC
NTHARAIEAGDAEAGCTVHEVTAALDDGPILGQARVPIQSDDTPEALAARILPLEHRLYP
CCHHHEEECCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCHHHHHHH
AVLRRFASGDRTKLKLS
HHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
SLRVAILISGGGSNMVALARDMVGHHPARPCLVVSNVPGAGGLAKAETMGIPTACVDHR
CEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHCCH
AFKGDRAAFEAALQKVLIAHTPGILCLAGFMRILTPDFVAGWEGQMLNIHPSLLPLYKGL
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHCCCCCCEEEECHHHHHHHHCC
NTHARAIEAGDAEAGCTVHEVTAALDDGPILGQARVPIQSDDTPEALAARILPLEHRLYP
CCHHHEEECCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCHHHHHHH
AVLRRFASGDRTKLKLS
HHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]