Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 89054298

GI number: 89054298

Start: 1795690

End: 1796520

Strand: Direct

Name: hemK [H]

Synonym: Jann_1807

Alternate gene names: 89054298

Gene position: 1795690-1796520 (Clockwise)

Preceding gene: 89054297

Following gene: 89054299

Centisome position: 41.59

GC content: 63.06

Gene sequence:

>831_bases
TTGAGGCACAGCCTTGAGCTTGCCCGAAAAGGGTTTTCCGATTTTTTGCCTGATGGGGAGGCCGAACGCGAAGCGCGGCT
TTTGATGTGTCACGCGCTAAAGATTTCTTCCGCCCAATTGTATGCGCGGTTGGATGAAACCTGGCCGACGGGCCCTCCCT
TTCAGCTGTTTGTCGAGGCCTGCGAGGCGCGCAAGTCCCGCCAACCGCTGAGCCAGATTATCGGTGAAGTGGAGTTCTAT
GGGCGCCGGTTTTTTGTGAACTCTGACGTGCTGACACCGCGCCCCGATACGGAAACGCTTGTCGATCAGGCCCTATCGGG
CGGTTTCGAGCGCGTGCTGGATCTTGGCACCGGGTCCGGGTGTGTTCTTGCGACGCTTTTGGCGGAGCGTCCGGGATCAA
ATGGGGTCGGCACTGATCTGTCGCAGCCCGCGCTGGAGGTGGCCGCGCGCAACGTCGCACGCCAAGGGGTGCAGGACCGC
GCGGTCTTGGTTCATTCGGATTGGTTCGAGGCGGTGCAGGGACAGTTCGACCTTATCCTCTCGAACCCTCCCTACATTGC
CGCAGCCGAGATGCGGGGATTGGCGCCCGAGGTTCGGGATTGGGAGCCGCATGTGGCCCTGACACCGGGCGGGGACGGTT
TGACCGCCTACCGCATCATCACGGCCGCGGGCCCCATGCATCTGCGCGCTGGTGGCCGTCTGATGGTCGAGATCGGCCTT
GATCAGGGCGCGGCGGTGGCAGAGCTGTTCCGCGCGGCAGGGTTTGGCGACGTCCGGATCACGCAGGACCTGAGCGGCCA
TGACCGTGTTGTTGGCGGAATCACGCCCTAA

Upstream 100 bases:

>100_bases
TGGATGCGGTGATGCTGGGGGATCTGGACGAAATCGTCGACGCCCTGACGGCAGAGGATCAGGCCATGAAACTGGCCGAG
ATGACCGGGTGACTCTTCTG

Downstream 100 bases:

>100_bases
AAACCGTTACATCTGATCGAAACCCCTGTTTTGTGCCAGATTAGGCTGGACTCGCCCGCACCCCCGTGCTTACTCAGTCC
CAAGTGCAGACAGCGGCCCG

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; M.RprHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRHSLELARKGFSDFLPDGEAEREARLLMCHALKISSAQLYARLDETWPTGPPFQLFVEACEARKSRQPLSQIIGEVEFY
GRRFFVNSDVLTPRPDTETLVDQALSGGFERVLDLGTGSGCVLATLLAERPGSNGVGTDLSQPALEVAARNVARQGVQDR
AVLVHSDWFEAVQGQFDLILSNPPYIAAAEMRGLAPEVRDWEPHVALTPGGDGLTAYRIITAAGPMHLRAGGRLMVEIGL
DQGAAVAELFRAAGFGDVRITQDLSGHDRVVGGITP

Sequences:

>Translated_276_residues
MRHSLELARKGFSDFLPDGEAEREARLLMCHALKISSAQLYARLDETWPTGPPFQLFVEACEARKSRQPLSQIIGEVEFY
GRRFFVNSDVLTPRPDTETLVDQALSGGFERVLDLGTGSGCVLATLLAERPGSNGVGTDLSQPALEVAARNVARQGVQDR
AVLVHSDWFEAVQGQFDLILSNPPYIAAAEMRGLAPEVRDWEPHVALTPGGDGLTAYRIITAAGPMHLRAGGRLMVEIGL
DQGAAVAELFRAAGFGDVRITQDLSGHDRVVGGITP
>Mature_276_residues
MRHSLELARKGFSDFLPDGEAEREARLLMCHALKISSAQLYARLDETWPTGPPFQLFVEACEARKSRQPLSQIIGEVEFY
GRRFFVNSDVLTPRPDTETLVDQALSGGFERVLDLGTGSGCVLATLLAERPGSNGVGTDLSQPALEVAARNVARQGVQDR
AVLVHSDWFEAVQGQFDLILSNPPYIAAAEMRGLAPEVRDWEPHVALTPGGDGLTAYRIITAAGPMHLRAGGRLMVEIGL
DQGAAVAELFRAAGFGDVRITQDLSGHDRVVGGITP

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=237, Percent_Identity=31.2236286919831, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=33.8289962825279, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082085, Length=180, Percent_Identity=32.2222222222222, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582226, Length=248, Percent_Identity=29.0322580645161, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 29831; Mature: 29831

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSLELARKGFSDFLPDGEAEREARLLMCHALKISSAQLYARLDETWPTGPPFQLFVEA
CCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHH
CEARKSRQPLSQIIGEVEFYGRRFFVNSDVLTPRPDTETLVDQALSGGFERVLDLGTGSG
HHHHHHCCHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHHCCCCCH
CVLATLLAERPGSNGVGTDLSQPALEVAARNVARQGVQDRAVLVHSDWFEAVQGQFDLIL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCEEEEE
SNPPYIAAAEMRGLAPEVRDWEPHVALTPGGDGLTAYRIITAAGPMHLRAGGRLMVEIGL
CCCCEEEHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCEEEECCCEEEEEECC
DQGAAVAELFRAAGFGDVRITQDLSGHDRVVGGITP
CCCHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCC
>Mature Secondary Structure
MRHSLELARKGFSDFLPDGEAEREARLLMCHALKISSAQLYARLDETWPTGPPFQLFVEA
CCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHH
CEARKSRQPLSQIIGEVEFYGRRFFVNSDVLTPRPDTETLVDQALSGGFERVLDLGTGSG
HHHHHHCCHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHHCCCCCH
CVLATLLAERPGSNGVGTDLSQPALEVAARNVARQGVQDRAVLVHSDWFEAVQGQFDLIL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCEEEEE
SNPPYIAAAEMRGLAPEVRDWEPHVALTPGGDGLTAYRIITAAGPMHLRAGGRLMVEIGL
CCCCEEEHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCEEEECCCEEEEEECC
DQGAAVAELFRAAGFGDVRITQDLSGHDRVVGGITP
CCCHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]