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The map label for this gene is prfA

Identifier: 89054297

GI number: 89054297

Start: 1794617

End: 1795681

Strand: Direct

Name: prfA

Synonym: Jann_1806

Alternate gene names: 89054297

Gene position: 1794617-1795681 (Clockwise)

Preceding gene: 89054296

Following gene: 89054298

Centisome position: 41.56

GC content: 63.85

Gene sequence:

>1065_bases
ATGGCCGCAACACTGCCCCGCGACCGCCTGCGACAGATCACGGATCGGTTCGAATATCTGGAAGCGCAGCTCAACGGCGG
GCCGGACCCTTCAGACATCGCCAAGATCAGCCGGGAATATGCGGAGCTGAAGCCGGTCGTCGCCGAGATTGCGGGGTACG
AGCAACTGCTGGCGGATATGGCCGAGGCGCAGAACATGCTGGCCGATCCTGACATGAGGCCCCTGGCGGAGGAGGAATTG
CCCCGCCTGGAAGCCGCCATCCCCGCCGCCGAGCAATCGCTGCAACTGGCGCTTCTGCCCAAGGATGCCGCCGACGCGAA
GCCTGCAATGATCGAGATCCGCCCTGGCACCGGCGGCGATGAGGCGGCTTTGTTCGCGGGTGATCTGTTGCGGATGTATA
CGCGCTATGCGGAGGCGCGCGGCTGGCGGTTGGAGATCGTGGATCTGCAGGAGAGTGATCTGGGCGGCATCAAGGAATGC
ACTGCGCGGGTGGAGGGGGAGAATGTCTTTGCCCGGCTGAAGTTTGAGAGTGGCGTCCACCGGGTCCAGCGCGTGCCGGA
AACGGAATCGGGCGGGCGTATCCATACCTCTGCCGCCACCGTCGCTGTGTTGCCGGAGGCCGAGGACGTCGACATTGACA
TCCCCGCCACCGATATTCGCATCGACACAATGCGCGCCAGCGGGGCGGGCGGGCAGCACGTGAACACCACGGATTCAGCG
GTGCGCATCACCCATGTGCCCTCTGGCATCGTGGTCGTCAGTTCCGAGAAGTCCCAGCACCGCAACCGGGAAATCGCGAT
GCAGACGCTGCGCACCCGGCTCTATGATCTGGAACGGCAGAAGGCGGATGATGCCATGGCCGCGGACCGCAAGGCGCAGA
TCGGCTCGGGCGATCGCTCGGAGCGGATCCGCACCTATAATTTCCCGCAAGGGCGCATGACCGATCACCGCATTAACCTG
ACACTCTATAAGCTGGATGCGGTGATGCTGGGGGATCTGGACGAAATCGTCGACGCCCTGACGGCAGAGGATCAGGCCAT
GAAACTGGCCGAGATGACCGGGTGA

Upstream 100 bases:

>100_bases
CTTCTCGCGCTCTCGGCACGGGATGAGCGATCTGGGCGTGAACACGGCGCGGGTTCAGCGTTGGTTGACGGCCGCCCGCA
ATGAAGAAAGCGGAACCTGA

Downstream 100 bases:

>100_bases
CTCTTCTGTTGAGGCACAGCCTTGAGCTTGCCCGAAAAGGGTTTTCCGATTTTTTGCCTGATGGGGAGGCCGAACGCGAA
GCGCGGCTTTTGATGTGTCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MAATLPRDRLRQITDRFEYLEAQLNGGPDPSDIAKISREYAELKPVVAEIAGYEQLLADMAEAQNMLADPDMRPLAEEEL
PRLEAAIPAAEQSLQLALLPKDAADAKPAMIEIRPGTGGDEAALFAGDLLRMYTRYAEARGWRLEIVDLQESDLGGIKEC
TARVEGENVFARLKFESGVHRVQRVPETESGGRIHTSAATVAVLPEAEDVDIDIPATDIRIDTMRASGAGGQHVNTTDSA
VRITHVPSGIVVVSSEKSQHRNREIAMQTLRTRLYDLERQKADDAMAADRKAQIGSGDRSERIRTYNFPQGRMTDHRINL
TLYKLDAVMLGDLDEIVDALTAEDQAMKLAEMTG

Sequences:

>Translated_354_residues
MAATLPRDRLRQITDRFEYLEAQLNGGPDPSDIAKISREYAELKPVVAEIAGYEQLLADMAEAQNMLADPDMRPLAEEEL
PRLEAAIPAAEQSLQLALLPKDAADAKPAMIEIRPGTGGDEAALFAGDLLRMYTRYAEARGWRLEIVDLQESDLGGIKEC
TARVEGENVFARLKFESGVHRVQRVPETESGGRIHTSAATVAVLPEAEDVDIDIPATDIRIDTMRASGAGGQHVNTTDSA
VRITHVPSGIVVVSSEKSQHRNREIAMQTLRTRLYDLERQKADDAMAADRKAQIGSGDRSERIRTYNFPQGRMTDHRINL
TLYKLDAVMLGDLDEIVDALTAEDQAMKLAEMTG
>Mature_353_residues
AATLPRDRLRQITDRFEYLEAQLNGGPDPSDIAKISREYAELKPVVAEIAGYEQLLADMAEAQNMLADPDMRPLAEEELP
RLEAAIPAAEQSLQLALLPKDAADAKPAMIEIRPGTGGDEAALFAGDLLRMYTRYAEARGWRLEIVDLQESDLGGIKECT
ARVEGENVFARLKFESGVHRVQRVPETESGGRIHTSAATVAVLPEAEDVDIDIPATDIRIDTMRASGAGGQHVNTTDSAV
RITHVPSGIVVVSSEKSQHRNREIAMQTLRTRLYDLERQKADDAMAADRKAQIGSGDRSERIRTYNFPQGRMTDHRINLT
LYKLDAVMLGDLDEIVDALTAEDQAMKLAEMTG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=43.8271604938272, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=355, Percent_Identity=36.9014084507042, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI166795305, Length=229, Percent_Identity=41.0480349344978, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1787462, Length=350, Percent_Identity=53.4285714285714, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI2367172, Length=333, Percent_Identity=36.6366366366366, Blast_Score=200, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=322, Percent_Identity=37.5776397515528, Blast_Score=197, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=42.8134556574924, Blast_Score=251, Evalue=9e-68,
Organism=Drosophila melanogaster, GI19921226, Length=344, Percent_Identity=41.5697674418605, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_JANSC (Q28RD9)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509748.1
- ProteinModelPortal:   Q28RD9
- SMR:   Q28RD9
- STRING:   Q28RD9
- GeneID:   3934255
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1806
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- PhylomeDB:   Q28RD9
- ProtClustDB:   PRK00591
- BioCyc:   JSP290400:JANN_1806-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38963; Mature: 38832

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATLPRDRLRQITDRFEYLEAQLNGGPDPSDIAKISREYAELKPVVAEIAGYEQLLADM
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEAQNMLADPDMRPLAEEELPRLEAAIPAAEQSLQLALLPKDAADAKPAMIEIRPGTGGD
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCC
EAALFAGDLLRMYTRYAEARGWRLEIVDLQESDLGGIKECTARVEGENVFARLKFESGVH
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEHHHHHH
RVQRVPETESGGRIHTSAATVAVLPEAEDVDIDIPATDIRIDTMRASGAGGQHVNTTDSA
HHHHCCCCCCCCEEEECCEEEEEECCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCE
VRITHVPSGIVVVSSEKSQHRNREIAMQTLRTRLYDLERQKADDAMAADRKAQIGSGDRS
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCCC
ERIRTYNFPQGRMTDHRINLTLYKLDAVMLGDLDEIVDALTAEDQAMKLAEMTG
CCCEEECCCCCCCCCEEEEEEEEEEEEHHHCCHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AATLPRDRLRQITDRFEYLEAQLNGGPDPSDIAKISREYAELKPVVAEIAGYEQLLADM
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEAQNMLADPDMRPLAEEELPRLEAAIPAAEQSLQLALLPKDAADAKPAMIEIRPGTGGD
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCC
EAALFAGDLLRMYTRYAEARGWRLEIVDLQESDLGGIKECTARVEGENVFARLKFESGVH
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEHHHHHH
RVQRVPETESGGRIHTSAATVAVLPEAEDVDIDIPATDIRIDTMRASGAGGQHVNTTDSA
HHHHCCCCCCCCEEEECCEEEEEECCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCE
VRITHVPSGIVVVSSEKSQHRNREIAMQTLRTRLYDLERQKADDAMAADRKAQIGSGDRS
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCCC
ERIRTYNFPQGRMTDHRINLTLYKLDAVMLGDLDEIVDALTAEDQAMKLAEMTG
CCCEEECCCCCCCCCEEEEEEEEEEEEHHHCCHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA