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The map label for this gene is rluE [H]

Identifier: 89054176

GI number: 89054176

Start: 1669181

End: 1669747

Strand: Direct

Name: rluE [H]

Synonym: Jann_1685

Alternate gene names: 89054176

Gene position: 1669181-1669747 (Clockwise)

Preceding gene: 89054175

Following gene: 89054177

Centisome position: 38.66

GC content: 63.32

Gene sequence:

>567_bases
ATGGTCAGACGCGCGCCGAGGGGGCACCATATGCTGATCGCGTTTAACAAACCGATGAACACGCTGTCTCAATTCACCTC
CGAGGGCGGCTGGCCCGCACTGGATGGGTTTGGCCTGCCCAAGGGCGTCTATGCCGCCGGGCGGCTGGATCGTGACAGTG
AGGGGCTGTTGCTGCTGACAGACGATGGCGGATTGCAGGCGCGGATATCCTCGCCGAAATACCGGTCTCCCAAGGTGTAT
TTCGCATTGGTCGAAGGCGTGCCCGATGACGCCGCGCTGGACCATTTGCGCCGGGGCGTGACGCTGAAGGACGGGCAAAC
GGCCCCATGCGAGGCCGAGGCGGTCGCAGCGCCCGATTGGATATGGGACCGGGATCCGCCAGTCCGCATTCGCAAATCCA
TCACCGACACGTGGCTAAAGCTGACATTGACGGAAGGCCGCAACCGTCAGGTGCGACGCATGTGCGCGGGCGTGGGTTTC
CCGGTGATCCGGCTGGTGCGGTTTTCCATTGGGCCCCATGATATCAGGGACTTACCCCCCGGCATGTGGCGGGAATTGGC
CCCATGA

Upstream 100 bases:

>100_bases
GACTGGGCCGAGGCGCGCCTGCGCCGAACGTGGCGGGACCTTGCGCCTAAGAAATGGCTCGCAGTCTACGACGCGCGCCA
GTGATGCGGGGGCCATACGG

Downstream 100 bases:

>100_bases
CCGAGGTGCTGACGGTCCGGTCCCGTGCGGAGTTGCGGGCATGGCTGGCAGAGCATCATGCCGGGGCGCGCGGCCTTTGG
CTGGCCACCTACAAGAAACA

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MVRRAPRGHHMLIAFNKPMNTLSQFTSEGGWPALDGFGLPKGVYAAGRLDRDSEGLLLLTDDGGLQARISSPKYRSPKVY
FALVEGVPDDAALDHLRRGVTLKDGQTAPCEAEAVAAPDWIWDRDPPVRIRKSITDTWLKLTLTEGRNRQVRRMCAGVGF
PVIRLVRFSIGPHDIRDLPPGMWRELAP

Sequences:

>Translated_188_residues
MVRRAPRGHHMLIAFNKPMNTLSQFTSEGGWPALDGFGLPKGVYAAGRLDRDSEGLLLLTDDGGLQARISSPKYRSPKVY
FALVEGVPDDAALDHLRRGVTLKDGQTAPCEAEAVAAPDWIWDRDPPVRIRKSITDTWLKLTLTEGRNRQVRRMCAGVGF
PVIRLVRFSIGPHDIRDLPPGMWRELAP
>Mature_188_residues
MVRRAPRGHHMLIAFNKPMNTLSQFTSEGGWPALDGFGLPKGVYAAGRLDRDSEGLLLLTDDGGLQARISSPKYRSPKVY
FALVEGVPDDAALDHLRRGVTLKDGQTAPCEAEAVAAPDWIWDRDPPVRIRKSITDTWLKLTLTEGRNRQVRRMCAGVGF
PVIRLVRFSIGPHDIRDLPPGMWRELAP

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=54.5454545454545, Blast_Score=206, Evalue=8e-55,
Organism=Escherichia coli, GI1788510, Length=147, Percent_Identity=38.0952380952381, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI1787524, Length=191, Percent_Identity=35.6020942408377, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790453, Length=179, Percent_Identity=30.1675977653631, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20921; Mature: 20921

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRAPRGHHMLIAFNKPMNTLSQFTSEGGWPALDGFGLPKGVYAAGRLDRDSEGLLLLT
CCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DDGGLQARISSPKYRSPKVYFALVEGVPDDAALDHLRRGVTLKDGQTAPCEAEAVAAPDW
CCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCCCCCCCCCCEECCCC
IWDRDPPVRIRKSITDTWLKLTLTEGRNRQVRRMCAGVGFPVIRLVRFSIGPHDIRDLPP
CCCCCCCHHHHHHHHCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCC
GMWRELAP
HHHHHCCC
>Mature Secondary Structure
MVRRAPRGHHMLIAFNKPMNTLSQFTSEGGWPALDGFGLPKGVYAAGRLDRDSEGLLLLT
CCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DDGGLQARISSPKYRSPKVYFALVEGVPDDAALDHLRRGVTLKDGQTAPCEAEAVAAPDW
CCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCCCCCCCCCCEECCCC
IWDRDPPVRIRKSITDTWLKLTLTEGRNRQVRRMCAGVGFPVIRLVRFSIGPHDIRDLPP
CCCCCCCHHHHHHHHCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCC
GMWRELAP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]