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The map label for this gene is nth [H]

Identifier: 89054160

GI number: 89054160

Start: 1653195

End: 1653917

Strand: Direct

Name: nth [H]

Synonym: Jann_1669

Alternate gene names: 89054160

Gene position: 1653195-1653917 (Clockwise)

Preceding gene: 89054156

Following gene: 89054161

Centisome position: 38.29

GC content: 60.72

Gene sequence:

>723_bases
ATGCTGAGGGGCTTGCCCGTCCGCGCCCGGCACGCCAAAAGGGACGCCATGGCCAAGCAACTGACCTACCACACGATACA
CGAGATTTTCACCCGCTTTCGCGACGCCGAAGCGGAGCCCAAGGGCGAGCTGCATCACGTCAATGTCTATACCCTCGTGG
TGGCCGTGGCGCTGTCGGCGCAGGCGACGGATGCGGGCGTGAACAAGGCGACGAAGCGGTTGTTTGAGATCGCCGATACA
CCGCAAAAGATGCTCGACCTGGGGTTGGAGGCGGTGACGGAGCATATCAGGACCATCGGCCTCTACCGCAATAAAGCCAA
GAACGTCATCAAGTTAAGCCAGATTCTGGTGGATGAATACGGCGGTGAAGTGCCGTCCTCCCGCACCGCCCTGCAATCGC
TGCCGGGTGTGGGGCGCAAGACCGCAAATGTGGTGTTAAACATGTGGTGGGGGATGCCGGCACAAGCCGTCGACACGCAT
ATTTTCCGGGTCGGCAACCGCACGCTGATCGCGCCGGGCAAGGATGTGGACGCTGTTGAGCGCGCGGTGGAAGACAACAT
CCCGGCCGAATTTCAACTGCATGCGCATCACTGGCTGATCCTCCATGGCCGCTACATCTGCGTGGCGCGGAAACCAAAAT
GCGGCGCCTGTCTGATCCGTGATCTATGCGTGTTCGAGGACAAGACGCCCGATCCGCTGCCGAAAAAAGGAGCGCGCCCA
TGA

Upstream 100 bases:

>100_bases
CGTGATTGGCGGGATGGGACATGGCTTGGATCTCCTTAACGCCGTTAACTTATCCAGCCCGGACCCGGTCTGCGACCCGG
TTCTTGCGGCTAAGACGGTG

Downstream 100 bases:

>100_bases
CGCGTCAGAACGTCTCTTCGGGGTCCTATCTGGAACCTCAGATCGGATTTTCGAGGGCCGTGCGCATTGGCGATATGATC
GCGGTGGGGGGCACGGCGCC

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLRGLPVRARHAKRDAMAKQLTYHTIHEIFTRFRDAEAEPKGELHHVNVYTLVVAVALSAQATDAGVNKATKRLFEIADT
PQKMLDLGLEAVTEHIRTIGLYRNKAKNVIKLSQILVDEYGGEVPSSRTALQSLPGVGRKTANVVLNMWWGMPAQAVDTH
IFRVGNRTLIAPGKDVDAVERAVEDNIPAEFQLHAHHWLILHGRYICVARKPKCGACLIRDLCVFEDKTPDPLPKKGARP

Sequences:

>Translated_240_residues
MLRGLPVRARHAKRDAMAKQLTYHTIHEIFTRFRDAEAEPKGELHHVNVYTLVVAVALSAQATDAGVNKATKRLFEIADT
PQKMLDLGLEAVTEHIRTIGLYRNKAKNVIKLSQILVDEYGGEVPSSRTALQSLPGVGRKTANVVLNMWWGMPAQAVDTH
IFRVGNRTLIAPGKDVDAVERAVEDNIPAEFQLHAHHWLILHGRYICVARKPKCGACLIRDLCVFEDKTPDPLPKKGARP
>Mature_240_residues
MLRGLPVRARHAKRDAMAKQLTYHTIHEIFTRFRDAEAEPKGELHHVNVYTLVVAVALSAQATDAGVNKATKRLFEIADT
PQKMLDLGLEAVTEHIRTIGLYRNKAKNVIKLSQILVDEYGGEVPSSRTALQSLPGVGRKTANVVLNMWWGMPAQAVDTH
IFRVGNRTLIAPGKDVDAVERAVEDNIPAEFQLHAHHWLILHGRYICVARKPKCGACLIRDLCVFEDKTPDPLPKKGARP

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=220, Percent_Identity=30.4545454545455, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=57.2139303482587, Blast_Score=251, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=33.1460674157303, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=28.4210526315789, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.0734463276836, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26743; Mature: 26743

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGLPVRARHAKRDAMAKQLTYHTIHEIFTRFRDAEAEPKGELHHVNVYTLVVAVALSA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHC
QATDAGVNKATKRLFEIADTPQKMLDLGLEAVTEHIRTIGLYRNKAKNVIKLSQILVDEY
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGEVPSSRTALQSLPGVGRKTANVVLNMWWGMPAQAVDTHIFRVGNRTLIAPGKDVDAVE
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEECCEEEECCCCCHHHHH
RAVEDNIPAEFQLHAHHWLILHGRYICVARKPKCGACLIRDLCVFEDKTPDPLPKKGARP
HHHHCCCCCEEEEECEEEEEEECCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRGLPVRARHAKRDAMAKQLTYHTIHEIFTRFRDAEAEPKGELHHVNVYTLVVAVALSA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHC
QATDAGVNKATKRLFEIADTPQKMLDLGLEAVTEHIRTIGLYRNKAKNVIKLSQILVDEY
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGEVPSSRTALQSLPGVGRKTANVVLNMWWGMPAQAVDTHIFRVGNRTLIAPGKDVDAVE
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEECCEEEECCCCCHHHHH
RAVEDNIPAEFQLHAHHWLILHGRYICVARKPKCGACLIRDLCVFEDKTPDPLPKKGARP
HHHHCCCCCEEEEECEEEEEEECCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]