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The map label for this gene is stcD [H]

Identifier: 89054019

GI number: 89054019

Start: 1496877

End: 1498922

Strand: Direct

Name: stcD [H]

Synonym: Jann_1528

Alternate gene names: 89054019

Gene position: 1496877-1498922 (Clockwise)

Preceding gene: 89054017

Following gene: 89054020

Centisome position: 34.67

GC content: 60.9

Gene sequence:

>2046_bases
ATGTCCACCGACCCGCTTCTTCAGCCGTTTCAGCTTAAACACCTCACCCTGCGCAACCGCATCATGACCACCAGTCATGA
ACCTGCCTATCCGCAGGACGGCATGCCCAAGGAACGCTACGCCGCCTATCACGCTGAGCGCGCCAAGGCCGGAGTGGCAC
TGGCGATGACGGCAGGATCGGCTGCGGTCAGCCGCGACAGCCCGCCGGTGTTCAACAACATCTTGGCCTATAAGGACGAG
GTCGTGCCGTGGATCCAGAACCTGACGGACGCGGTGCATGAACACGACTGCGCGGTGATGATCCAGCTGACGCACCTCGG
GCGGCGGACCAACTGGAACAAAGGGGATTGGTTGCCATCTGTGTCATCCTCCAAGCACCGCGAACCTGCGCACCGCGCCT
ACCCAAAGCTGATTGAGGATTGGGACATTGACCGCATTATCAATGATTTTGCCGATGCTGCTGAGCGCATGAAAGCGGGC
GGCATGGACGGGATCGAATTGCAGGTCTACGGCCACCTGCTCGATCAATTCTGGTCACCGCTGACCAATGATCTGGTGGG
GCCATACGGGGCCGATACGCTGGAAAACCGTATGCGGTTCCCGATGGATGTGGTGGAGGCGATACGCAAACGCGTGGGCA
CAGAGTTCATCGTGGGCCTACGCTACACGGCGGATGAGGCGCAGAAAGGCGGGATTACTCCCGACGAAGGTTTGGAAATC
TCCAAACGCCTTGCCGCGACGGGGCAGGTGGATTTCCTGAACGTCATCAAAGGGCGCATCCATACCGATCCGGCCATGAC
TGACATGATCCCTGTTCAGGGCATGAAATCCGCCCCGCACCTCGATTTCGCGGGCGAGGTCCGCAAGACCACAGGGATGC
CCACCTTCCACGCCGCTCGAATTCCCGATGTGGCGACCGCACGGCACGCCGTTCAAGCGGGTCTGCTGGATATGGTGGGC
ATGACCCGCGCCCATATGGCTGATCCGCATGTGGTTCGCAAAATCATCGAAGGGCGCGAGGATGATATCCGCCCCTGCGT
CGGCGCGACTTACTGCCTAGATCGCATCTATCAGGCGGGCGAAGCGCTTTGCCTCCACAACGCCGCCACGGGCCGTGAGT
TGTCCATGCCTCATGATATTCCCACGACGGACGCCAAGAAGAAGGTCGTTATCATCGGTGCAGGCCCCGCAGGACTGGAA
GCCGCGCGCGTCGCGGCGGAGCGCGGCCATGACGTGACCGTGTTGGAGGCTGCGCCAGAGGCTGGCGGGCAGGTGCGCCT
GACAGCACGCTCCCCCCGCCGTCGCGAGATGATCGGCATCATCGACTGGCGTATGGCGCAATGCGCCGCCCGCGATGTGA
CGTTCCATTTCAACACATGGGCCGAAGCGGGCGATGTGACGGCATTGAACCCCGATGTGGTGATTGTTGCAACTGGCGGC
CTGCCGAACCTGGAATTGTTCGAGCAGAAGGGCGAGCAGGCGCATGTTGTGTCCACGTGGGATATCATCTCGGGCGATGT
TAAACCTGCGGTTAAGGTGCTGATCTATGATGAAAGCGGCGACCACCCCGCGCTGCAAGCCGCTGAAATTGCGGCCAATG
CGGGGTCGTCTGTCGAAATTATGACGCCGGATCGCACTTTCTCACCCGATGTAATGGCGATGAATCTGGTGCCCTACATG
CGCTCGCTGCAAGACAAGGACGTGACCTTTACCGTCACGCGCCGCTTGATGGGGGTCGTGCGGAGCGGCGACACATTGAC
CGCAACAATCGGCACCGATTACAGCGACCACACGTATGCGGCCGAGGTCGATCAGGTCGTCGTTAACTACGGCACCCTGC
CGCTGGATGACCTGTATTTCGACCTCAAACCATCGTCCTCCAACCTCGGCGAGGTGGACTACGACGCGCTGATCGCAGGC
ACCTCGCAGACGGTCCACAGCAACGACACCGGCACCTTCAAACTGTTCCGCATCGGCGATGCCGTGTCGGCCCGCAACAC
CCACGCCGCCATCTACGACGCCCTGCGTCTGGTTAAGGATCTCTGA

Upstream 100 bases:

>100_bases
GTTCATACCGCGAGACTACGGAACTTGACAGGTGTGTCAATTGGCGGAAGTGTCGGCTTGACACCTATGTCAGGTCAGAG
CCGCCTAAGAAGGATCCGCC

Downstream 100 bases:

>100_bases
AATGCGCAGCTCTAAGACCATCCACGTCGTGTCTTGCCATGCCGAAGGCGAAGTGGGCGATGTGATCGTCGGCGGCGTCG
CTCCGCCCCCCGGCAAAACC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MSTDPLLQPFQLKHLTLRNRIMTTSHEPAYPQDGMPKERYAAYHAERAKAGVALAMTAGSAAVSRDSPPVFNNILAYKDE
VVPWIQNLTDAVHEHDCAVMIQLTHLGRRTNWNKGDWLPSVSSSKHREPAHRAYPKLIEDWDIDRIINDFADAAERMKAG
GMDGIELQVYGHLLDQFWSPLTNDLVGPYGADTLENRMRFPMDVVEAIRKRVGTEFIVGLRYTADEAQKGGITPDEGLEI
SKRLAATGQVDFLNVIKGRIHTDPAMTDMIPVQGMKSAPHLDFAGEVRKTTGMPTFHAARIPDVATARHAVQAGLLDMVG
MTRAHMADPHVVRKIIEGREDDIRPCVGATYCLDRIYQAGEALCLHNAATGRELSMPHDIPTTDAKKKVVIIGAGPAGLE
AARVAAERGHDVTVLEAAPEAGGQVRLTARSPRRREMIGIIDWRMAQCAARDVTFHFNTWAEAGDVTALNPDVVIVATGG
LPNLELFEQKGEQAHVVSTWDIISGDVKPAVKVLIYDESGDHPALQAAEIAANAGSSVEIMTPDRTFSPDVMAMNLVPYM
RSLQDKDVTFTVTRRLMGVVRSGDTLTATIGTDYSDHTYAAEVDQVVVNYGTLPLDDLYFDLKPSSSNLGEVDYDALIAG
TSQTVHSNDTGTFKLFRIGDAVSARNTHAAIYDALRLVKDL

Sequences:

>Translated_681_residues
MSTDPLLQPFQLKHLTLRNRIMTTSHEPAYPQDGMPKERYAAYHAERAKAGVALAMTAGSAAVSRDSPPVFNNILAYKDE
VVPWIQNLTDAVHEHDCAVMIQLTHLGRRTNWNKGDWLPSVSSSKHREPAHRAYPKLIEDWDIDRIINDFADAAERMKAG
GMDGIELQVYGHLLDQFWSPLTNDLVGPYGADTLENRMRFPMDVVEAIRKRVGTEFIVGLRYTADEAQKGGITPDEGLEI
SKRLAATGQVDFLNVIKGRIHTDPAMTDMIPVQGMKSAPHLDFAGEVRKTTGMPTFHAARIPDVATARHAVQAGLLDMVG
MTRAHMADPHVVRKIIEGREDDIRPCVGATYCLDRIYQAGEALCLHNAATGRELSMPHDIPTTDAKKKVVIIGAGPAGLE
AARVAAERGHDVTVLEAAPEAGGQVRLTARSPRRREMIGIIDWRMAQCAARDVTFHFNTWAEAGDVTALNPDVVIVATGG
LPNLELFEQKGEQAHVVSTWDIISGDVKPAVKVLIYDESGDHPALQAAEIAANAGSSVEIMTPDRTFSPDVMAMNLVPYM
RSLQDKDVTFTVTRRLMGVVRSGDTLTATIGTDYSDHTYAAEVDQVVVNYGTLPLDDLYFDLKPSSSNLGEVDYDALIAG
TSQTVHSNDTGTFKLFRIGDAVSARNTHAAIYDALRLVKDL
>Mature_680_residues
STDPLLQPFQLKHLTLRNRIMTTSHEPAYPQDGMPKERYAAYHAERAKAGVALAMTAGSAAVSRDSPPVFNNILAYKDEV
VPWIQNLTDAVHEHDCAVMIQLTHLGRRTNWNKGDWLPSVSSSKHREPAHRAYPKLIEDWDIDRIINDFADAAERMKAGG
MDGIELQVYGHLLDQFWSPLTNDLVGPYGADTLENRMRFPMDVVEAIRKRVGTEFIVGLRYTADEAQKGGITPDEGLEIS
KRLAATGQVDFLNVIKGRIHTDPAMTDMIPVQGMKSAPHLDFAGEVRKTTGMPTFHAARIPDVATARHAVQAGLLDMVGM
TRAHMADPHVVRKIIEGREDDIRPCVGATYCLDRIYQAGEALCLHNAATGRELSMPHDIPTTDAKKKVVIIGAGPAGLEA
ARVAAERGHDVTVLEAAPEAGGQVRLTARSPRRREMIGIIDWRMAQCAARDVTFHFNTWAEAGDVTALNPDVVIVATGGL
PNLELFEQKGEQAHVVSTWDIISGDVKPAVKVLIYDESGDHPALQAAEIAANAGSSVEIMTPDRTFSPDVMAMNLVPYMR
SLQDKDVTFTVTRRLMGVVRSGDTLTATIGTDYSDHTYAAEVDQVVVNYGTLPLDDLYFDLKPSSSNLGEVDYDALIAGT
SQTVHSNDTGTFKLFRIGDAVSARNTHAAIYDALRLVKDL

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=492, Percent_Identity=31.3008130081301, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1787939, Length=359, Percent_Identity=25.3481894150418, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17559802, Length=273, Percent_Identity=28.9377289377289, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565138, Length=336, Percent_Identity=24.702380952381, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=254, Percent_Identity=28.3464566929134, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=276, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=258, Percent_Identity=26.7441860465116, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=361, Percent_Identity=26.5927977839335, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=368, Percent_Identity=23.0978260869565, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74583; Mature: 74452

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDPLLQPFQLKHLTLRNRIMTTSHEPAYPQDGMPKERYAAYHAERAKAGVALAMTAGS
CCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCC
AAVSRDSPPVFNNILAYKDEVVPWIQNLTDAVHEHDCAVMIQLTHLGRRTNWNKGDWLPS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCC
VSSSKHREPAHRAYPKLIEDWDIDRIINDFADAAERMKAGGMDGIELQVYGHLLDQFWSP
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
LTNDLVGPYGADTLENRMRFPMDVVEAIRKRVGTEFIVGLRYTADEAQKGGITPDEGLEI
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEEECCHHHCCCCCCCCCCHH
SKRLAATGQVDFLNVIKGRIHTDPAMTDMIPVQGMKSAPHLDFAGEVRKTTGMPTFHAAR
HHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEECCC
IPDVATARHAVQAGLLDMVGMTRAHMADPHVVRKIIEGREDDIRPCVGATYCLDRIYQAG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
EALCLHNAATGRELSMPHDIPTTDAKKKVVIIGAGPAGLEAARVAAERGHDVTVLEAAPE
CEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCC
AGGQVRLTARSPRRREMIGIIDWRMAQCAARDVTFHFNTWAEAGDVTALNPDVVIVATGG
CCCEEEEEECCCCHHHHEEHHHHHHHHHHHHCEEEEEECCCCCCCEEEECCCEEEEECCC
LPNLELFEQKGEQAHVVSTWDIISGDVKPAVKVLIYDESGDHPALQAAEIAANAGSSVEI
CCCHHHHHHCCCEEEEEEEEHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEEEE
MTPDRTFSPDVMAMNLVPYMRSLQDKDVTFTVTRRLMGVVRSGDTLTATIGTDYSDHTYA
ECCCCCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHH
AEVDQVVVNYGTLPLDDLYFDLKPSSSNLGEVDYDALIAGTSQTVHSNDTGTFKLFRIGD
HHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCHHEEECCCCEEECCCCCEEEEEEECC
AVSARNTHAAIYDALRLVKDL
CCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STDPLLQPFQLKHLTLRNRIMTTSHEPAYPQDGMPKERYAAYHAERAKAGVALAMTAGS
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCC
AAVSRDSPPVFNNILAYKDEVVPWIQNLTDAVHEHDCAVMIQLTHLGRRTNWNKGDWLPS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCC
VSSSKHREPAHRAYPKLIEDWDIDRIINDFADAAERMKAGGMDGIELQVYGHLLDQFWSP
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
LTNDLVGPYGADTLENRMRFPMDVVEAIRKRVGTEFIVGLRYTADEAQKGGITPDEGLEI
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEEECCHHHCCCCCCCCCCHH
SKRLAATGQVDFLNVIKGRIHTDPAMTDMIPVQGMKSAPHLDFAGEVRKTTGMPTFHAAR
HHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEECCC
IPDVATARHAVQAGLLDMVGMTRAHMADPHVVRKIIEGREDDIRPCVGATYCLDRIYQAG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
EALCLHNAATGRELSMPHDIPTTDAKKKVVIIGAGPAGLEAARVAAERGHDVTVLEAAPE
CEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCC
AGGQVRLTARSPRRREMIGIIDWRMAQCAARDVTFHFNTWAEAGDVTALNPDVVIVATGG
CCCEEEEEECCCCHHHHEEHHHHHHHHHHHHCEEEEEECCCCCCCEEEECCCEEEEECCC
LPNLELFEQKGEQAHVVSTWDIISGDVKPAVKVLIYDESGDHPALQAAEIAANAGSSVEI
CCCHHHHHHCCCEEEEEEEEHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEEEE
MTPDRTFSPDVMAMNLVPYMRSLQDKDVTFTVTRRLMGVVRSGDTLTATIGTDYSDHTYA
ECCCCCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHH
AEVDQVVVNYGTLPLDDLYFDLKPSSSNLGEVDYDALIAGTSQTVHSNDTGTFKLFRIGD
HHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCHHEEECCCCEEECCCCCEEEEEEECC
AVSARNTHAAIYDALRLVKDL
CCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]