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The map label for this gene is ahpF [C]

Identifier: 89053798

GI number: 89053798

Start: 1267236

End: 1268258

Strand: Reverse

Name: ahpF [C]

Synonym: Jann_1307

Alternate gene names: 89053798

Gene position: 1268258-1267236 (Counterclockwise)

Preceding gene: 89053800

Following gene: 89053797

Centisome position: 29.37

GC content: 57.48

Gene sequence:

>1023_bases
ATGTGGCGAGTTGACAACTTGGACGACGAAAAGACTCTGATGGCCATGAACACCCACAAAAGCACGGAAGTTTCTAACGT
GATCGACGTGGCGATCATCGGCGGCAGCTTCGCGGGTTTGTCTGCCGCCTATCCGCTTGTGCGGGCACGGCGAAGCACCG
TGATGTTCCATACCGGCGAGACCCGAAACCGGTTCTCTGCCGAAAGTCACAACTGGATCGGGCTGGACGGCGTTGGCCCG
GAAAGCGTCCATCAGTCAGGAATTCACGAACTGGCTAAGTATTCCGATTTCTCGATCGTGAAAGATCGGGTGGTCCGAGC
GCAGCGGTCCGAGACACATCAACACCTCGGGGTGCCGATCACCGAGCTGACAACAGCCTTGGGGAGCACCATCCATGCAC
GGCGCGTTATCTTGGCGACTGGGATGCGGGATACATTTCCCTTGGATATCGAAGGGTTGGAAGACTGCTGGGGTCAGTCC
GTACTGCAATGCCCGTATTGTCATGGATACGAACATCGTGACGTGCCAACGGGCATCCTGCACGGTGGTCCGCCATCTCT
GCATCAAGCGAAGAACCTGCCGGATTTCACGAACGATCTGGTTTATTTCACCAACGGGGCCGAGATCGAGGCCTCGGACA
TCGCAGATCTGGAAGCGCGCGGATACCGCGTTGAGACTGGGGCTGTGAGCAGCATTGAGCACCATGACGGAGCGATGAGT
GCCGTGGCCCTACGTGGCGGCACGAAAATCGCAAGCGAAGCGCTCTACATCATTCCTCGCGCAGCATTCGCAACCGACCT
ACATGCACAGTTGGGATGTGAAGAAATCGAAGGGCCTTTCGGACCCTATGTTACCGTGGACGATCTGCACGAGACCACGC
AGCGCGGTGTTTTCGCCGCAGGAGACATGACACGTCCTGCCTTCAATGCCGTCTGGGCAGGAGCAGACGGGAATCGTGCA
GGAATTTTCGCGCATCAGTCGCTGGTATCAGAGCATAACCCATACCGCGGAGCGCGACTTTGA

Upstream 100 bases:

>100_bases
CCCAAGGGCGTGCAGGGCCAGAGACAATTTGCTGTTTCGCTTCATGCAACCAATCTAGTTGCATTTTTTTCAAAAATAAA
GTAACTGCCTTGGTTGCGAT

Downstream 100 bases:

>100_bases
AAGTGTGAAGCGGCCTTTCGTTGCAAGAACAGTATTCCGTAGGTCTGGGCTCTCTGCGGCCATTAGATCGCACGCAGCAT
CTATACAGCCACGACCCAAA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: Thioredoxin Reductase; Oxidoreductase; FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; Thioredoxin-Disulfide Reductase; Reductase; Pyridine Nucleotide-Disulphide Oxidoreductase Class II; FAD Dependent Oxidoreductase; Pyridine Nucleotide-Disulphide Oxidoreductase; Pyridine Nucleotide-Disulfide Oxidoreductase; Thioredoxin Reductase Protein; Pyridine Nucleotide-Disulfide Oxidoreductase Domain Protein; Methyltransferase Type; Fad-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; Alkyl Hydroperoxide Reductase; Pyridine Nucleotide-Disulfide Oxidoreductase Family Protein; Oxidoreductase Protein; Methyltransferase; Alkyl Hydroperoxide Reductase Subunit F; Disulfide Oxidoreductase; Alkyl Hydroperoxide Reductase Large Subunit

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MWRVDNLDDEKTLMAMNTHKSTEVSNVIDVAIIGGSFAGLSAAYPLVRARRSTVMFHTGETRNRFSAESHNWIGLDGVGP
ESVHQSGIHELAKYSDFSIVKDRVVRAQRSETHQHLGVPITELTTALGSTIHARRVILATGMRDTFPLDIEGLEDCWGQS
VLQCPYCHGYEHRDVPTGILHGGPPSLHQAKNLPDFTNDLVYFTNGAEIEASDIADLEARGYRVETGAVSSIEHHDGAMS
AVALRGGTKIASEALYIIPRAAFATDLHAQLGCEEIEGPFGPYVTVDDLHETTQRGVFAAGDMTRPAFNAVWAGADGNRA
GIFAHQSLVSEHNPYRGARL

Sequences:

>Translated_340_residues
MWRVDNLDDEKTLMAMNTHKSTEVSNVIDVAIIGGSFAGLSAAYPLVRARRSTVMFHTGETRNRFSAESHNWIGLDGVGP
ESVHQSGIHELAKYSDFSIVKDRVVRAQRSETHQHLGVPITELTTALGSTIHARRVILATGMRDTFPLDIEGLEDCWGQS
VLQCPYCHGYEHRDVPTGILHGGPPSLHQAKNLPDFTNDLVYFTNGAEIEASDIADLEARGYRVETGAVSSIEHHDGAMS
AVALRGGTKIASEALYIIPRAAFATDLHAQLGCEEIEGPFGPYVTVDDLHETTQRGVFAAGDMTRPAFNAVWAGADGNRA
GIFAHQSLVSEHNPYRGARL
>Mature_340_residues
MWRVDNLDDEKTLMAMNTHKSTEVSNVIDVAIIGGSFAGLSAAYPLVRARRSTVMFHTGETRNRFSAESHNWIGLDGVGP
ESVHQSGIHELAKYSDFSIVKDRVVRAQRSETHQHLGVPITELTTALGSTIHARRVILATGMRDTFPLDIEGLEDCWGQS
VLQCPYCHGYEHRDVPTGILHGGPPSLHQAKNLPDFTNDLVYFTNGAEIEASDIADLEARGYRVETGAVSSIEHHDGAMS
AVALRGGTKIASEALYIIPRAAFATDLHAQLGCEEIEGPFGPYVTVDDLHETTQRGVFAAGDMTRPAFNAVWAGADGNRA
GIFAHQSLVSEHNPYRGARL

Specific function: Serves To Protect The Cell Against DNA Damage By Alkyl Hydroperoxides. It Can Use Either NADH Or NADPH As Electron Donor For Direct Reduction Of Redox Dyes Or Of Alkyl Hydroperoxides When Combined With The Ahpc Protein. [C]

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.6.4.- [C]

Molecular weight: Translated: 37012; Mature: 37012

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRVDNLDDEKTLMAMNTHKSTEVSNVIDVAIIGGSFAGLSAAYPLVRARRSTVMFHTGE
CCCCCCCCCCCEEEEECCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCC
TRNRFSAESHNWIGLDGVGPESVHQSGIHELAKYSDFSIVKDRVVRAQRSETHQHLGVPI
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCH
TELTTALGSTIHARRVILATGMRDTFPLDIEGLEDCWGQSVLQCPYCHGYEHRDVPTGIL
HHHHHHHCCHHHHHEEEEECCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCCEE
HGGPPSLHQAKNLPDFTNDLVYFTNGAEIEASDIADLEARGYRVETGAVSSIEHHDGAMS
CCCCCCHHHHCCCCCCCCCEEEEECCCEEECCCCCHHHHCCEEEECCCCCCHHHCCCCEE
AVALRGGTKIASEALYIIPRAAFATDLHAQLGCEEIEGPFGPYVTVDDLHETTQRGVFAA
EEEECCCCHHHCCEEEEEECHHHHHHHHHHCCHHHHCCCCCCEEEHHHHHHHHHCCEEEE
GDMTRPAFNAVWAGADGNRAGIFAHQSLVSEHNPYRGARL
CCCCCCHHHEEEECCCCCCCEEHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MWRVDNLDDEKTLMAMNTHKSTEVSNVIDVAIIGGSFAGLSAAYPLVRARRSTVMFHTGE
CCCCCCCCCCCEEEEECCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCC
TRNRFSAESHNWIGLDGVGPESVHQSGIHELAKYSDFSIVKDRVVRAQRSETHQHLGVPI
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCH
TELTTALGSTIHARRVILATGMRDTFPLDIEGLEDCWGQSVLQCPYCHGYEHRDVPTGIL
HHHHHHHCCHHHHHEEEEECCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCCEE
HGGPPSLHQAKNLPDFTNDLVYFTNGAEIEASDIADLEARGYRVETGAVSSIEHHDGAMS
CCCCCCHHHHCCCCCCCCCEEEEECCCEEECCCCCHHHHCCEEEECCCCCCHHHCCCCEE
AVALRGGTKIASEALYIIPRAAFATDLHAQLGCEEIEGPFGPYVTVDDLHETTQRGVFAA
EEEECCCCHHHCCEEEEEECHHHHHHHHHHCCHHHHCCCCCCEEEHHHHHHHHHCCEEEE
GDMTRPAFNAVWAGADGNRAGIFAHQSLVSEHNPYRGARL
CCCCCCHHHEEEECCCCCCCEEHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA