Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 89053235

GI number: 89053235

Start: 713070

End: 713897

Strand: Reverse

Name: trmD [H]

Synonym: Jann_0744

Alternate gene names: 89053235

Gene position: 713897-713070 (Counterclockwise)

Preceding gene: 89053236

Following gene: 89053234

Centisome position: 16.53

GC content: 65.46

Gene sequence:

>828_bases
ATGACCCAGTCACCCTCCAAATCATCCCCCTCCAAATCCCATGGTCGCCTGTCCCAATCGGCAAGCCTCAAGCCTCGGGA
GTTGATGACCGAGCATCCACGGCTTGCGAAAGCCTGGTGCGCCAAGGTCATCACGCTGTTCCCGGAAACATTCCCCGGCG
TCTTGGGCGCGTCGCTGACCGGAAAGGCCCTGCAAAAGGGTCTGTGGGCGTTGGAGCCGATCGACCTGCGGACTTTCGGC
ACGGGCAAGCACCGGCAAGTGGACGACACGCCCGCAGGCGGCGGTGCGGGGCTGGTCCTAAAGCCCGACGTGATGGCCCG
CGCTTTGGATATCGCCGCACGCGGCACGCCTGCGGATCGGGCGGACTGGCCCATCGTCTACCTCTCCCCGCGCGGCAAAC
CGTTTGAGCAGCGCGATGCCGAACGGTTCGCGAGTGCCAAGGGCATCACGCTTGTCTGCGGCCGGTTCGAGGGCGTCGAT
CAGCGGGTCATCGACGCCTACGGGATGGAGGAGATCTGCGTCGGCGATGCCGTTCTGACGGGCGGAGAGATCGCCGCGCA
ACTGGTGCTGGATGCCACAACACGGCTGATCCCGGGCGTGTTGGGCAACGCGGACTCGACGCAAGAGGAGAGCTTTTCCG
ACGGGTTGCTGGAACATCCGCAATACACCAAGCCCGCCGACTGGCGCGGCCATACGATCCCCCCGGTGTTGCTGTCGGGC
GATCACGGGAAAGTGGCGGAATGGCGGAAGGCGCAGGCCGAGGCGCTGACGCAGGAGCGGCGGCCAGACTTGTGGGTCAA
GGTGGCCAAACCCAAGAAGCCTCGGTGA

Upstream 100 bases:

>100_bases
TGCGGGCGAGGATGACGGCGGCATCGTAATCGACAATGCCCGCTTTTCGGTCGCGCCGTAAATGTTTGATCCTGTTATGT
CGCCCGCGTAAACGGACGGC

Downstream 100 bases:

>100_bases
TGGAACCCCCAAACCTTCTCACCATGTTAAGTGTTGCCTCATCACTTCCGCTATCAATCTGCCTCTTTGCATTCAATAAG
AGGTACCCTCGGTTAGCTTG

Product: tRNA (Guanine37-N1) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTQSPSKSSPSKSHGRLSQSASLKPRELMTEHPRLAKAWCAKVITLFPETFPGVLGASLTGKALQKGLWALEPIDLRTFG
TGKHRQVDDTPAGGGAGLVLKPDVMARALDIAARGTPADRADWPIVYLSPRGKPFEQRDAERFASAKGITLVCGRFEGVD
QRVIDAYGMEEICVGDAVLTGGEIAAQLVLDATTRLIPGVLGNADSTQEESFSDGLLEHPQYTKPADWRGHTIPPVLLSG
DHGKVAEWRKAQAEALTQERRPDLWVKVAKPKKPR

Sequences:

>Translated_275_residues
MTQSPSKSSPSKSHGRLSQSASLKPRELMTEHPRLAKAWCAKVITLFPETFPGVLGASLTGKALQKGLWALEPIDLRTFG
TGKHRQVDDTPAGGGAGLVLKPDVMARALDIAARGTPADRADWPIVYLSPRGKPFEQRDAERFASAKGITLVCGRFEGVD
QRVIDAYGMEEICVGDAVLTGGEIAAQLVLDATTRLIPGVLGNADSTQEESFSDGLLEHPQYTKPADWRGHTIPPVLLSG
DHGKVAEWRKAQAEALTQERRPDLWVKVAKPKKPR
>Mature_274_residues
TQSPSKSSPSKSHGRLSQSASLKPRELMTEHPRLAKAWCAKVITLFPETFPGVLGASLTGKALQKGLWALEPIDLRTFGT
GKHRQVDDTPAGGGAGLVLKPDVMARALDIAARGTPADRADWPIVYLSPRGKPFEQRDAERFASAKGITLVCGRFEGVDQ
RVIDAYGMEEICVGDAVLTGGEIAAQLVLDATTRLIPGVLGNADSTQEESFSDGLLEHPQYTKPADWRGHTIPPVLLSGD
HGKVAEWRKAQAEALTQERRPDLWVKVAKPKKPR

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=228, Percent_Identity=40.3508771929825, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29772; Mature: 29641

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSPSKSSPSKSHGRLSQSASLKPRELMTEHPRLAKAWCAKVITLFPETFPGVLGASLT
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
GKALQKGLWALEPIDLRTFGTGKHRQVDDTPAGGGAGLVLKPDVMARALDIAARGTPADR
HHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCC
ADWPIVYLSPRGKPFEQRDAERFASAKGITLVCGRFEGVDQRVIDAYGMEEICVGDAVLT
CCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCHHHHHCCHHCC
GGEIAAQLVLDATTRLIPGVLGNADSTQEESFSDGLLEHPQYTKPADWRGHTIPPVLLSG
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCEEEEC
DHGKVAEWRKAQAEALTQERRPDLWVKVAKPKKPR
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TQSPSKSSPSKSHGRLSQSASLKPRELMTEHPRLAKAWCAKVITLFPETFPGVLGASLT
CCCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
GKALQKGLWALEPIDLRTFGTGKHRQVDDTPAGGGAGLVLKPDVMARALDIAARGTPADR
HHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCC
ADWPIVYLSPRGKPFEQRDAERFASAKGITLVCGRFEGVDQRVIDAYGMEEICVGDAVLT
CCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCHHHHHCCHHCC
GGEIAAQLVLDATTRLIPGVLGNADSTQEESFSDGLLEHPQYTKPADWRGHTIPPVLLSG
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCEEEEC
DHGKVAEWRKAQAEALTQERRPDLWVKVAKPKKPR
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA