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The map label for this gene is def [H]

Identifier: 89052957

GI number: 89052957

Start: 465992

End: 466516

Strand: Direct

Name: def [H]

Synonym: Jann_0466

Alternate gene names: 89052957

Gene position: 465992-466516 (Clockwise)

Preceding gene: 89052955

Following gene: 89052958

Centisome position: 10.79

GC content: 62.1

Gene sequence:

>525_bases
ATGAAGCTTCCGATCCTGATCCATCCCGACCCGCGCCTGAAGAAGGTCGCCGCGCCCGTGCCTGATGTCACGGACGAGTT
GCGCGCCTTGGCCGACAATATGCTCAGCACGATGTACGACGCCCCCGGCATTGGCCTGGCTGCGCCGCAAGTGGGCGTGG
GGCAGCGGTTGATCGTGCTGGATTGCGAGAAGGGCGATGATGTCACGCCGCGTCCATTGGCGATGTTCAACCCAGAGGTC
ATCGCCTCCTCCGACGAGATGAACACCTATGATGAAGGGTGCCTGTCGATCCCCGATATCTATGCCGACGTGACCCGGCC
TGAGGCCGTGACCGTCCGGTGGATGGATGTGAACGGGGCTGAGCAGGAAGAGACGTTTGACGGCCTGTGGGCCACCTGCG
TGCAGCACGAGATCGATCATCTGGAGGGAAAGCTTTTCATCGACTACCTCAGCGGGCTGAAGCGGCAGCTGATCACGCGC
AAGATGGTGAAGTTGAAGCGCGACCGAGCGCGCGAAGGCGCGTGA

Upstream 100 bases:

>100_bases
CGTGGGGCTGCAAGGGCGGCGTCCGTTCGGATTAGATAATTGAAGCCCGGGACGGCAGAGGGTCTTTCCCGGGGACGTTG
TTTCGCATACATCCACGCCC

Downstream 100 bases:

>100_bases
GTGTCCGGCGGGTCCTGCTGTGGCCCGATCCGCTGTTGTCGGTCGTTTGCGATGCGGTCGTGGCCGTTGATCCCGAGTTG
ATCAAAGACCTGTTCGATAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKLPILIHPDPRLKKVAAPVPDVTDELRALADNMLSTMYDAPGIGLAAPQVGVGQRLIVLDCEKGDDVTPRPLAMFNPEV
IASSDEMNTYDEGCLSIPDIYADVTRPEAVTVRWMDVNGAEQEETFDGLWATCVQHEIDHLEGKLFIDYLSGLKRQLITR
KMVKLKRDRAREGA

Sequences:

>Translated_174_residues
MKLPILIHPDPRLKKVAAPVPDVTDELRALADNMLSTMYDAPGIGLAAPQVGVGQRLIVLDCEKGDDVTPRPLAMFNPEV
IASSDEMNTYDEGCLSIPDIYADVTRPEAVTVRWMDVNGAEQEETFDGLWATCVQHEIDHLEGKLFIDYLSGLKRQLITR
KMVKLKRDRAREGA
>Mature_174_residues
MKLPILIHPDPRLKKVAAPVPDVTDELRALADNMLSTMYDAPGIGLAAPQVGVGQRLIVLDCEKGDDVTPRPLAMFNPEV
IASSDEMNTYDEGCLSIPDIYADVTRPEAVTVRWMDVNGAEQEETFDGLWATCVQHEIDHLEGKLFIDYLSGLKRQLITR
KMVKLKRDRAREGA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=33.1210191082803, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=49.112426035503, Blast_Score=150, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=31.2101910828025, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=33.1210191082803, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19360; Mature: 19360

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPILIHPDPRLKKVAAPVPDVTDELRALADNMLSTMYDAPGIGLAAPQVGVGQRLIVL
CCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DCEKGDDVTPRPLAMFNPEVIASSDEMNTYDEGCLSIPDIYADVTRPEAVTVRWMDVNGA
ECCCCCCCCCCCEEHCCCHHEECCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCC
EQEETFDGLWATCVQHEIDHLEGKLFIDYLSGLKRQLITRKMVKLKRDRAREGA
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLPILIHPDPRLKKVAAPVPDVTDELRALADNMLSTMYDAPGIGLAAPQVGVGQRLIVL
CCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DCEKGDDVTPRPLAMFNPEVIASSDEMNTYDEGCLSIPDIYADVTRPEAVTVRWMDVNGA
ECCCCCCCCCCCEEHCCCHHEECCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCC
EQEETFDGLWATCVQHEIDHLEGKLFIDYLSGLKRQLITRKMVKLKRDRAREGA
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]