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The map label for this gene is rho

Identifier: 89052689

GI number: 89052689

Start: 189047

End: 190255

Strand: Direct

Name: rho

Synonym: Jann_0198

Alternate gene names: 89052689

Gene position: 189047-190255 (Clockwise)

Preceding gene: 89052684

Following gene: 89052690

Centisome position: 4.38

GC content: 54.84

Gene sequence:

>1209_bases
ATGGCGGAAGAGCTTGAGATCGAGAACGCTTCCACCATGCGGAAGGGGGATATGATGTTCGAGATCCTCCGAGAGCGGGC
TGACGAGGGTTGGGAGATCTCCGGTGACGGTGTGTTGGAAGTGTTGCAGGATGGGTTCGGATTCTTACGCAGTCCTGAGG
CCAACTACTTGGCCGGGCCCGACGATATCTACGTCTCACCGGATATGATCCGACAACACTCGCTGCGTACGGGTGACACC
GTTGAAGGCGTCATGTCCGCACCTAAGGAAAACGAGCGGTATTTCTGCCTGGTGAACGTCACGCAGATCAACTTTGAAGA
ACCTGAGAAGGCAAAGCACAAGGTAGCGTTTGATAACCTGACACCGCTGTATCCCGATCAGCGGATGAACATGGAGATTG
AGGATCCCACGATCAAGGACCGGTCTGCCCGAATTATTGATCTGGTGTCGCCGATCGGTAAGGGGCAGCGAAGCCTGATT
GTGGCACCGCCGCGGACGGGCAAGACGGTTCTGCTTCAGAATATTGCTCATTCGATCGAGACCAATCACCCGGAGTGCTA
TCTGATCGTACTTCTGATCGACGAGCGGCCGGAAGAGGTCACCGATATGCAGCGCTCGGTCAAGGGAGAGGTGATTTCCT
CCACCTTTGATGAGCCCGCGACCCGCCACGTGGCTGTCTCGGAGATGGTGATCGAGAAGGCAAAACGTCTGGTGGAGCAC
AAGCGGGACGTTGTGATCTTGCTGGACTCAATCACGCGCTTGGGTCGAGCTTTCAACACGGTTGTTCCCTCTTCTGGCAA
GGTTCTGACCGGTGGTGTTGATGCGAACGCCCTGCAGCGCCCGAAGCGGTTCTTCGGTGCAGCGCGGAATATTGAGGAAG
GTGGATCGTTGACGATCATCGCGACGGCATTGATCGATACAGGAAGCCGGATGGACGAAGTGATCTTTGAGGAGTTCAAA
GGAACGGGCAACAGCGAGATCGTTCTGGATCGCAAGATTGCCGACAAGCGCGTGTATCCTGCCATGGACATTCTGAAGTC
CGGCACGCGGAAGGAAGATCTTCTCGTGGACAAGGGCGACTTGCAGAAAACGTTTGTGTTGCGGCGCATTCTGAACCCGA
TGGGCACGACGGACGCGATCGAATTCCTGATTTCCAAGTTGAAACAGACAAAAACGAACTCAGAATTCTTCGATTCCATG
AATACCTGA

Upstream 100 bases:

>100_bases
TGAGACGCATGATGATCGTGTCCACTTTGGATATCCCATGACACAACAAACGCTTCGTCTTGCTGATTTGAAAGCGAAAG
CTGCCAAGGATCTTCTGCCC

Downstream 100 bases:

>100_bases
GGAGTGTGTCGTGCAGACGATTTTCGCTCAGGCAACGGCCCGGGGGAAAGCGGGCGTTGCGATTATTCGAATTTCGGGAC
CAGATGCGTTTGAAGTTGGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAEELEIENASTMRKGDMMFEILRERADEGWEISGDGVLEVLQDGFGFLRSPEANYLAGPDDIYVSPDMIRQHSLRTGDT
VEGVMSAPKENERYFCLVNVTQINFEEPEKAKHKVAFDNLTPLYPDQRMNMEIEDPTIKDRSARIIDLVSPIGKGQRSLI
VAPPRTGKTVLLQNIAHSIETNHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPATRHVAVSEMVIEKAKRLVEH
KRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFK
GTGNSEIVLDRKIADKRVYPAMDILKSGTRKEDLLVDKGDLQKTFVLRRILNPMGTTDAIEFLISKLKQTKTNSEFFDSM
NT

Sequences:

>Translated_402_residues
MAEELEIENASTMRKGDMMFEILRERADEGWEISGDGVLEVLQDGFGFLRSPEANYLAGPDDIYVSPDMIRQHSLRTGDT
VEGVMSAPKENERYFCLVNVTQINFEEPEKAKHKVAFDNLTPLYPDQRMNMEIEDPTIKDRSARIIDLVSPIGKGQRSLI
VAPPRTGKTVLLQNIAHSIETNHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPATRHVAVSEMVIEKAKRLVEH
KRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFK
GTGNSEIVLDRKIADKRVYPAMDILKSGTRKEDLLVDKGDLQKTFVLRRILNPMGTTDAIEFLISKLKQTKTNSEFFDSM
NT
>Mature_401_residues
AEELEIENASTMRKGDMMFEILRERADEGWEISGDGVLEVLQDGFGFLRSPEANYLAGPDDIYVSPDMIRQHSLRTGDTV
EGVMSAPKENERYFCLVNVTQINFEEPEKAKHKVAFDNLTPLYPDQRMNMEIEDPTIKDRSARIIDLVSPIGKGQRSLIV
APPRTGKTVLLQNIAHSIETNHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPATRHVAVSEMVIEKAKRLVEHK
RDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKG
TGNSEIVLDRKIADKRVYPAMDILKSGTRKEDLLVDKGDLQKTFVLRRILNPMGTTDAIEFLISKLKQTKTNSEFFDSMN
T

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=400, Percent_Identity=67.75, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 45061; Mature: 44930

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEELEIENASTMRKGDMMFEILRERADEGWEISGDGVLEVLQDGFGFLRSPEANYLAGP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCC
DDIYVSPDMIRQHSLRTGDTVEGVMSAPKENERYFCLVNVTQINFEEPEKAKHKVAFDNL
CCEEECHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHHHHEECCCC
TPLYPDQRMNMEIEDPTIKDRSARIIDLVSPIGKGQRSLIVAPPRTGKTVLLQNIAHSIE
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHC
TNHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPATRHVAVSEMVIEKAKRLVEH
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
KRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTII
CCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
ATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVYPAMDILKSGTRKEDLLVDKGD
EEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCHHHHHHHCCCCCCCEEECCCC
LQKTFVLRRILNPMGTTDAIEFLISKLKQTKTNSEFFDSMNT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
AEELEIENASTMRKGDMMFEILRERADEGWEISGDGVLEVLQDGFGFLRSPEANYLAGP
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCC
DDIYVSPDMIRQHSLRTGDTVEGVMSAPKENERYFCLVNVTQINFEEPEKAKHKVAFDNL
CCEEECHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHHHHEECCCC
TPLYPDQRMNMEIEDPTIKDRSARIIDLVSPIGKGQRSLIVAPPRTGKTVLLQNIAHSIE
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHC
TNHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPATRHVAVSEMVIEKAKRLVEH
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
KRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTII
CCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
ATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVYPAMDILKSGTRKEDLLVDKGD
EEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCHHHHHHHCCCCCCCEEECCCC
LQKTFVLRRILNPMGTTDAIEFLISKLKQTKTNSEFFDSMNT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]