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The map label for this gene is ilvI [H]

Identifier: 89052628

GI number: 89052628

Start: 127409

End: 129025

Strand: Reverse

Name: ilvI [H]

Synonym: Jann_0137

Alternate gene names: 89052628

Gene position: 129025-127409 (Counterclockwise)

Preceding gene: 89052630

Following gene: 89052626

Centisome position: 2.99

GC content: 61.84

Gene sequence:

>1617_bases
ATGACCAAGCACCACGGTGGAAAAATCCTTGCGGATCGGTTGGCGCTGCATGGGGTGGAGCGTGTGTTTTCCGTCCCCGG
GGAGAGTTTTCTGGCCGCGCTGGACGGGCTCTATGACCATGATATCCCGAACATTGTCTGCCGCCAGGAAGGCGGCGCTG
CGATGATGGCAGAGGCCCACGGGAAGATGACGGGGACTCCCGGCGTGCTGTTTGTCACGCGCGGACCGGGGGCCACGAAT
GCAAGTGCGGGCCTTCATATCGCCATGCATGACGCGACGCCTCTGGTGTGTTTCGTGGGGCAGATCCCCCTGAAGCACCG
TGATAGGGGTGTATTTCAAGAAGTTGATTACAGGGCGTTTTTCGGACCTTTGGTAAAGTGGTCGGCGGAGGTGGAGCGCA
CGGATCGCCTGGCCGAATACATTGACCGGGCGTTTGCGGTGGCCCAGGCCGGGCGACCCGGCCCGGTGGTTCTGGCCCTG
CCTGAGGATATCCTGTGTGCGATGGCCGAGCCGCCAAAACAACGCCCCGGTGCCCGCGCGATGGGACGCGTACACCCCGA
CGATATCGCGCCGATGGTGGAGGCGTTTAAGCTGGCCAAACGCCCCCTGATCCTTTGCGGTGGGCCCCATTGGGGTGACG
TGGAGAAGCAAACCGCGCAGGCCTTGGCTGAACGGACAGGCGCGCCGATTGCGGTGACGTTCCGGCGGCAGGACTACATC
GACAACGACCACCCCAACTATGCCGGAGATCTGGGCGTCGGTGCGAACCCTGCGCTACAAAAACGGCTCGGCGATGCGGA
TTGTGTCCTGCTGCTCGGGGCCGAACTCAACGATATCACGACCGCCGATTTCACTCTGATCGACCCCGCTGATCCGCCCA
CGATTTTGCAGGTGGCACCCACGCCAGAGGCGACGTCCAAAACCTATCCCGCGACCCTTGCCGTCGCCGCCCCGCCTATA
TCAATGCTATATCAATTTCTTGATGCAACCCCGCAGAGCCCGGACCGTGGGGCAAACGCGGCGCGGGGGGAGTTTGAGAA
ATGGCAGGTCCCACAGCTCACCCCCGGCGCGGTTCAGATGGAGAGCATTATCTGCTGGCTCCGCGATAACGCGAGCTCCG
ACACGATCATCACCAATGGCGCGGGGAACTACGCCGCCTTCCTGCATCGTTATTACCGGTTCCGTCAGTACGGGACGCAG
GTGGCGCCAACATCCGGCTCCATGGGTTACGGTCTGCCCGCGGCCGTATCCGCCAAATTGCACAATCCCGACCAGCCGGT
GATCTGCCTGGCGGGTGACGGATGTTTGCAGATGACAATTCAGGAGCTGTCGACCGCGCGGCAGCACGGGGCGGATATCA
TCGTGATCGTCGCCAATAACGGGCGCTATGGGACGATCCGCATGCATCAGGAAAACCACTATCCGGGGCGTGTGTCAGGG
ACGGATTTGTTCAACCCCGATTACGCCGCTTTGGCCGAGGCCTATGGGGGCACCGGGCATCTGGTGACAGAGAATGCTCA
ATTCGCAGACGCCTATCGGCAAGCCCTGACGGGCGGCTTGCACATCATCGAACTGGCGCTTGATCCGAAGATGCTGGCCA
CCACCAAAAGTCTCTAA

Upstream 100 bases:

>100_bases
GCCGCCCGGAGCGAGCCACATAGTCCCAAAAGTCTGCCGCTGGACATTGCAGCCCCTCCGTTTCACTAATCTTTCGACGC
ACATAATCCGAGAGCCTCCC

Downstream 100 bases:

>100_bases
TCTTCCTGTTTGTCCAACATCTTGATTTCTCGCTGCGGGAATGGGATGGAGATGTTGTTGGCGCTGAACGTATCCCACAA
CGCCAGGTAGACGTTGCCGC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTKHHGGKILADRLALHGVERVFSVPGESFLAALDGLYDHDIPNIVCRQEGGAAMMAEAHGKMTGTPGVLFVTRGPGATN
ASAGLHIAMHDATPLVCFVGQIPLKHRDRGVFQEVDYRAFFGPLVKWSAEVERTDRLAEYIDRAFAVAQAGRPGPVVLAL
PEDILCAMAEPPKQRPGARAMGRVHPDDIAPMVEAFKLAKRPLILCGGPHWGDVEKQTAQALAERTGAPIAVTFRRQDYI
DNDHPNYAGDLGVGANPALQKRLGDADCVLLLGAELNDITTADFTLIDPADPPTILQVAPTPEATSKTYPATLAVAAPPI
SMLYQFLDATPQSPDRGANAARGEFEKWQVPQLTPGAVQMESIICWLRDNASSDTIITNGAGNYAAFLHRYYRFRQYGTQ
VAPTSGSMGYGLPAAVSAKLHNPDQPVICLAGDGCLQMTIQELSTARQHGADIIVIVANNGRYGTIRMHQENHYPGRVSG
TDLFNPDYAALAEAYGGTGHLVTENAQFADAYRQALTGGLHIIELALDPKMLATTKSL

Sequences:

>Translated_538_residues
MTKHHGGKILADRLALHGVERVFSVPGESFLAALDGLYDHDIPNIVCRQEGGAAMMAEAHGKMTGTPGVLFVTRGPGATN
ASAGLHIAMHDATPLVCFVGQIPLKHRDRGVFQEVDYRAFFGPLVKWSAEVERTDRLAEYIDRAFAVAQAGRPGPVVLAL
PEDILCAMAEPPKQRPGARAMGRVHPDDIAPMVEAFKLAKRPLILCGGPHWGDVEKQTAQALAERTGAPIAVTFRRQDYI
DNDHPNYAGDLGVGANPALQKRLGDADCVLLLGAELNDITTADFTLIDPADPPTILQVAPTPEATSKTYPATLAVAAPPI
SMLYQFLDATPQSPDRGANAARGEFEKWQVPQLTPGAVQMESIICWLRDNASSDTIITNGAGNYAAFLHRYYRFRQYGTQ
VAPTSGSMGYGLPAAVSAKLHNPDQPVICLAGDGCLQMTIQELSTARQHGADIIVIVANNGRYGTIRMHQENHYPGRVSG
TDLFNPDYAALAEAYGGTGHLVTENAQFADAYRQALTGGLHIIELALDPKMLATTKSL
>Mature_537_residues
TKHHGGKILADRLALHGVERVFSVPGESFLAALDGLYDHDIPNIVCRQEGGAAMMAEAHGKMTGTPGVLFVTRGPGATNA
SAGLHIAMHDATPLVCFVGQIPLKHRDRGVFQEVDYRAFFGPLVKWSAEVERTDRLAEYIDRAFAVAQAGRPGPVVLALP
EDILCAMAEPPKQRPGARAMGRVHPDDIAPMVEAFKLAKRPLILCGGPHWGDVEKQTAQALAERTGAPIAVTFRRQDYID
NDHPNYAGDLGVGANPALQKRLGDADCVLLLGAELNDITTADFTLIDPADPPTILQVAPTPEATSKTYPATLAVAAPPIS
MLYQFLDATPQSPDRGANAARGEFEKWQVPQLTPGAVQMESIICWLRDNASSDTIITNGAGNYAAFLHRYYRFRQYGTQV
APTSGSMGYGLPAAVSAKLHNPDQPVICLAGDGCLQMTIQELSTARQHGADIIVIVANNGRYGTIRMHQENHYPGRVSGT
DLFNPDYAALAEAYGGTGHLVTENAQFADAYRQALTGGLHIIELALDPKMLATTKSL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=23.5188509874327, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI93004078, Length=579, Percent_Identity=22.4525043177893, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI87081685, Length=548, Percent_Identity=29.5620437956204, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1790104, Length=489, Percent_Identity=29.4478527607362, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=26.007326007326, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=22.7611940298507, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788716, Length=564, Percent_Identity=23.5815602836879, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17542570, Length=577, Percent_Identity=25.1299826689775, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=546, Percent_Identity=26.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=546, Percent_Identity=26.1904761904762, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=27.5491949910555, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323163, Length=504, Percent_Identity=24.6031746031746, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320816, Length=156, Percent_Identity=27.5641025641026, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=26.4912280701754, Blast_Score=119, Evalue=8e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57779; Mature: 57648

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHHGGKILADRLALHGVERVFSVPGESFLAALDGLYDHDIPNIVCRQEGGAAMMAEAH
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCC
GKMTGTPGVLFVTRGPGATNASAGLHIAMHDATPLVCFVGQIPLKHRDRGVFQEVDYRAF
CCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHCCHHHH
FGPLVKWSAEVERTDRLAEYIDRAFAVAQAGRPGPVVLALPEDILCAMAEPPKQRPGARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCHH
MGRVHPDDIAPMVEAFKLAKRPLILCGGPHWGDVEKQTAQALAERTGAPIAVTFRRQDYI
CCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
DNDHPNYAGDLGVGANPALQKRLGDADCVLLLGAELNDITTADFTLIDPADPPTILQVAP
CCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEC
TPEATSKTYPATLAVAAPPISMLYQFLDATPQSPDRGANAARGEFEKWQVPQLTPGAVQM
CCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEH
ESIICWLRDNASSDTIITNGAGNYAAFLHRYYRFRQYGTQVAPTSGSMGYGLPAAVSAKL
HHEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHEEEEC
HNPDQPVICLAGDGCLQMTIQELSTARQHGADIIVIVANNGRYGTIRMHQENHYPGRVSG
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
TDLFNPDYAALAEAYGGTGHLVTENAQFADAYRQALTGGLHIIELALDPKMLATTKSL
CCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCEEEEEEECCCHHHHHCCCC
>Mature Secondary Structure 
TKHHGGKILADRLALHGVERVFSVPGESFLAALDGLYDHDIPNIVCRQEGGAAMMAEAH
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCC
GKMTGTPGVLFVTRGPGATNASAGLHIAMHDATPLVCFVGQIPLKHRDRGVFQEVDYRAF
CCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHCCHHHH
FGPLVKWSAEVERTDRLAEYIDRAFAVAQAGRPGPVVLALPEDILCAMAEPPKQRPGARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCHH
MGRVHPDDIAPMVEAFKLAKRPLILCGGPHWGDVEKQTAQALAERTGAPIAVTFRRQDYI
CCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
DNDHPNYAGDLGVGANPALQKRLGDADCVLLLGAELNDITTADFTLIDPADPPTILQVAP
CCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEC
TPEATSKTYPATLAVAAPPISMLYQFLDATPQSPDRGANAARGEFEKWQVPQLTPGAVQM
CCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEH
ESIICWLRDNASSDTIITNGAGNYAAFLHRYYRFRQYGTQVAPTSGSMGYGLPAAVSAKL
HHEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHEEEEC
HNPDQPVICLAGDGCLQMTIQELSTARQHGADIIVIVANNGRYGTIRMHQENHYPGRVSG
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
TDLFNPDYAALAEAYGGTGHLVTENAQFADAYRQALTGGLHIIELALDPKMLATTKSL
CCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCEEEEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]