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The map label for this gene is leuC

Identifier: 89052626

GI number: 89052626

Start: 124403

End: 125809

Strand: Reverse

Name: leuC

Synonym: Jann_0135

Alternate gene names: 89052626

Gene position: 125809-124403 (Counterclockwise)

Preceding gene: 89052628

Following gene: 89052617

Centisome position: 2.91

GC content: 63.18

Gene sequence:

>1407_bases
ATGACCGGCAAGACGCTTTACGACAAAATCTGGGACGCTCACCTGGCCCATGAGGCCGAAGACGGCACCAGCCTTCTCTA
TATTGACCGCCATCTGGTCCACGAAGTGACCAGCCCCCAGGCGTTTGAGGGTTTGCGTATGGCGGGCCGTTCGGTTCACG
CGCCCGACAAGACCATCGCCGTGCCGGACCACAATGTGCCGACGACGGAAGGCCGGGACGACCCCAAGAACATGACCGAG
GACAGCGCCATTCAGGTGGCGGCTTTGGACAAGAACGCCAAGGATTTCGGGATTCACTATTACCCCGTGTCCGATGTACG
CCAAGGCATCGTGCATATCGTGGGGCCGGAACAGGGCTGGACGCTTCCGGGCATGACGGTTGTGTGCGGGGATAGCCACA
CGGCCACCCACGGCGCGTTTGGCGCGCTGGCCCATGGCATCGGCACATCCGAGGTGGAGCATGTTCTGGCGACGCAAACG
CTGATCCAGAAGAAGTCCAAGAACATGAAGGTGGAGATCACGGGCAAACTGGCGCCCGGCGTCACCGCCAAGGACATCAC
GCTGACCGTCATTGGCCGCACAGGCACGGCGGGCGGCACCGGCTATGTGATTGAATATTGCGGCGAGGCGATCCGCGACC
TGTCCATGGAAGGGCGGATGACCGTCTGCAACATGGCGATTGAAGGCGGCGCGCGGGCGGGCATCATTGCGCCTGACGAG
AAGACGTTTGAATACTGTATGGGTCGCCCGCACGCGCCGAAAGGGGCGCAATGGGAGGCCGCTTTGGCGTGGTGGAAGAC
GCTGTATTCCGACGACGACGCCCATTGGGACGAGGTCGTGACGATCCGCGGCGAGGATATCGCGCCTGTGGTCACCTGGG
GCACCTCGCCCGAGGATGTTTTGCCCATCACGGCGGAGGTTCCCGCGGCAGACAGCTTTGAGGGTGGCAAGGTCGGTGCG
GCACAGCGATCACTGGACTACATGGGACTGACGGCGGGCACGCCGCTGGATCAGATCGAGATCGACACGGTGTTCATCGG
GTCCTGCACCAACGGACGGATTGAAGATCTGCGGGCTGCGGCCTCGATCCTGAAGGGCAAGAAGGTCAAGGACGGCCTGC
GGGCGATGATTGTGCCGGGCTCGGGCCTTGTGCGGGCGCAGGCCGAGGAAGAAGGCTTGGCCGATATCTTCAAGGATGCT
GGCTTTGAGTGGCGTCTTGCGGGCTGTTCCATGTGCCTCGCGATGAACCCCGATCAGCTGTCGCCTGGAGAGCGGTGCGC
GGCCACGTCCAACCGCAACTTTGAAGGTCGCCAGGGCCGTGGAGGACGGACGCATCTGATGTCACCGGCGATGGCTGCCG
CCGCCGCGATCACAGGCCGCCTGACGGATGTGCGCGAGTTGATGTAG

Upstream 100 bases:

>100_bases
CGCTTTTTTGGCGCGCAGGCCTCGCGTCTGGGCGGGCTATAGCGCACCCGCTTCGATATCACCCAGCCAGACACCTTATA
TTCCGACAGGATCCCTGCCC

Downstream 100 bases:

>100_bases
GGCGAGGGGACCTCTACGCTACGTCTCGACAGGTTCCGGAACGAACCGCCGGGCGGTGCGGTCCCGCCCCAGGCTGAGGC
AGAGGATCACAACGGGCAGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTGKTLYDKIWDAHLAHEAEDGTSLLYIDRHLVHEVTSPQAFEGLRMAGRSVHAPDKTIAVPDHNVPTTEGRDDPKNMTE
DSAIQVAALDKNAKDFGIHYYPVSDVRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQT
LIQKKSKNMKVEITGKLAPGVTAKDITLTVIGRTGTAGGTGYVIEYCGEAIRDLSMEGRMTVCNMAIEGGARAGIIAPDE
KTFEYCMGRPHAPKGAQWEAALAWWKTLYSDDDAHWDEVVTIRGEDIAPVVTWGTSPEDVLPITAEVPAADSFEGGKVGA
AQRSLDYMGLTAGTPLDQIEIDTVFIGSCTNGRIEDLRAAASILKGKKVKDGLRAMIVPGSGLVRAQAEEEGLADIFKDA
GFEWRLAGCSMCLAMNPDQLSPGERCAATSNRNFEGRQGRGGRTHLMSPAMAAAAAITGRLTDVRELM

Sequences:

>Translated_468_residues
MTGKTLYDKIWDAHLAHEAEDGTSLLYIDRHLVHEVTSPQAFEGLRMAGRSVHAPDKTIAVPDHNVPTTEGRDDPKNMTE
DSAIQVAALDKNAKDFGIHYYPVSDVRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQT
LIQKKSKNMKVEITGKLAPGVTAKDITLTVIGRTGTAGGTGYVIEYCGEAIRDLSMEGRMTVCNMAIEGGARAGIIAPDE
KTFEYCMGRPHAPKGAQWEAALAWWKTLYSDDDAHWDEVVTIRGEDIAPVVTWGTSPEDVLPITAEVPAADSFEGGKVGA
AQRSLDYMGLTAGTPLDQIEIDTVFIGSCTNGRIEDLRAAASILKGKKVKDGLRAMIVPGSGLVRAQAEEEGLADIFKDA
GFEWRLAGCSMCLAMNPDQLSPGERCAATSNRNFEGRQGRGGRTHLMSPAMAAAAAITGRLTDVRELM
>Mature_467_residues
TGKTLYDKIWDAHLAHEAEDGTSLLYIDRHLVHEVTSPQAFEGLRMAGRSVHAPDKTIAVPDHNVPTTEGRDDPKNMTED
SAIQVAALDKNAKDFGIHYYPVSDVRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTL
IQKKSKNMKVEITGKLAPGVTAKDITLTVIGRTGTAGGTGYVIEYCGEAIRDLSMEGRMTVCNMAIEGGARAGIIAPDEK
TFEYCMGRPHAPKGAQWEAALAWWKTLYSDDDAHWDEVVTIRGEDIAPVVTWGTSPEDVLPITAEVPAADSFEGGKVGAA
QRSLDYMGLTAGTPLDQIEIDTVFIGSCTNGRIEDLRAAASILKGKKVKDGLRAMIVPGSGLVRAQAEEEGLADIFKDAG
FEWRLAGCSMCLAMNPDQLSPGERCAATSNRNFEGRQGRGGRTHLMSPAMAAAAAITGRLTDVRELM

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=475, Percent_Identity=28.8421052631579, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI8659555, Length=383, Percent_Identity=25.065274151436, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=27.0833333333333, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=64.7311827956989, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.0387811634349, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081781, Length=366, Percent_Identity=25.4098360655738, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI2367097, Length=375, Percent_Identity=26.4, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=478, Percent_Identity=29.9163179916318, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=367, Percent_Identity=32.1525885558583, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=309, Percent_Identity=32.3624595469256, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=372, Percent_Identity=25.5376344086022, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=62.4203821656051, Blast_Score=597, Evalue=1e-171,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=30.6849315068493, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=484, Percent_Identity=27.8925619834711, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=458, Percent_Identity=24.6724890829694, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365315, Length=480, Percent_Identity=27.9166666666667, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17864292, Length=480, Percent_Identity=27.9166666666667, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076999, Length=362, Percent_Identity=29.8342541436464, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=27.0440251572327, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=26.8292682926829, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137564, Length=368, Percent_Identity=27.1739130434783, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_JANSC (Q28W60)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508077.1
- ProteinModelPortal:   Q28W60
- SMR:   Q28W60
- STRING:   Q28W60
- GeneID:   3932572
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0135
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q28W60
- ProtClustDB:   PRK05478
- BioCyc:   JSP290400:JANN_0135-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50063; Mature: 49932

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKTLYDKIWDAHLAHEAEDGTSLLYIDRHLVHEVTSPQAFEGLRMAGRSVHAPDKTIA
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEE
VPDHNVPTTEGRDDPKNMTEDSAIQVAALDKNAKDFGIHYYPVSDVRQGIVHIVGPEQGW
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEHHHHHCCEEEEECCCCCC
TLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTLIQKKSKNMKVEITGKLAPG
CCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
VTAKDITLTVIGRTGTAGGTGYVIEYCGEAIRDLSMEGRMTVCNMAIEGGARAGIIAPDE
CEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEECCCC
KTFEYCMGRPHAPKGAQWEAALAWWKTLYSDDDAHWDEVVTIRGEDIAPVVTWGTSPEDV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCCCE
LPITAEVPAADSFEGGKVGAAQRSLDYMGLTAGTPLDQIEIDTVFIGSCTNGRIEDLRAA
EEEEECCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHHH
ASILKGKKVKDGLRAMIVPGSGLVRAQAEEEGLADIFKDAGFEWRLAGCSMCLAMNPDQL
HHHHCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCC
SPGERCAATSNRNFEGRQGRGGRTHLMSPAMAAAAAITGRLTDVRELM
CCCCHHCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TGKTLYDKIWDAHLAHEAEDGTSLLYIDRHLVHEVTSPQAFEGLRMAGRSVHAPDKTIA
CCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEE
VPDHNVPTTEGRDDPKNMTEDSAIQVAALDKNAKDFGIHYYPVSDVRQGIVHIVGPEQGW
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEHHHHHCCEEEEECCCCCC
TLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTLIQKKSKNMKVEITGKLAPG
CCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
VTAKDITLTVIGRTGTAGGTGYVIEYCGEAIRDLSMEGRMTVCNMAIEGGARAGIIAPDE
CEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEECCCC
KTFEYCMGRPHAPKGAQWEAALAWWKTLYSDDDAHWDEVVTIRGEDIAPVVTWGTSPEDV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCCCE
LPITAEVPAADSFEGGKVGAAQRSLDYMGLTAGTPLDQIEIDTVFIGSCTNGRIEDLRAA
EEEEECCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHHH
ASILKGKKVKDGLRAMIVPGSGLVRAQAEEEGLADIFKDAGFEWRLAGCSMCLAMNPDQL
HHHHCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCC
SPGERCAATSNRNFEGRQGRGGRTHLMSPAMAAAAAITGRLTDVRELM
CCCCHHCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA