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The map label for this gene is ilvA [H]

Identifier: 89052560

GI number: 89052560

Start: 63420

End: 64697

Strand: Direct

Name: ilvA [H]

Synonym: Jann_0069

Alternate gene names: 89052560

Gene position: 63420-64697 (Clockwise)

Preceding gene: 89052559

Following gene: 89052562

Centisome position: 1.47

GC content: 60.09

Gene sequence:

>1278_bases
TTGACCGCCGGGACGGGCCAAGCCGTTGGCATGACAGATTTTGCCATTGCCGCCAAAGCCACGACCGCCGCGCTCAGGGA
CCTGTTCCCTGCCACGCCGCTGTTGAAAAACGCCTATCTGAGCGAGCGTTTTGGCGCGGACATTTGGCTGAAGCGCGAAG
ATCTTAGCCCGGTCCGCTCCTACAAGTTGCGTGGTGCGTTCAACGCCATGCGCAAGGTGCGCGCGGAGCGGCCCGAATTG
GTGGATTTCGTCTGTGCCAGCGCGGGCAATCACGCCCAGGGCGTGGCCTATGTCTGTGCCCATTTCGGCGTGCGCGGCAG
GGTTTACATGCCGGTCACAACGCCTGATCAAAAGATTCTGAAAACGCGCACTTTCGGGGGCGATGCCGTAGAGATTGTGT
TGGAAGGCGATTTCTTCGATGAAACCCAGGCCGCTGCGAAAGCCTATTGCGCAGAGGTCGGCGCCCATTTTCTGTCGCCC
TTCGACGATCCGGATGTGATCGAGGGGCAAGCCTCCGTCGCGGTTGAGATGTTGGAGCAGTTGGGTCGCGCGCCCGATCA
TCTGGTGCTGCCCGTAGGTGGCGGTGGCCTGTCGTCGGGCATGCTGTCCTACCTTCGGGACGTCGGGGCTGACACCCACG
CAACGCTGGTGGAACCTGATGGCGGGCCTAGCCTCACCGCAGCCCTGAAGATCGGCGCGCCGCAAACCATCGAAATTACA
GACACATTCGTGGATGGCGCCGCTGTGGCGCGCATAGGCAATCGCAACTTCGAGGTGCTGCGAAACGTCCCGCCCCGCGA
CGTGCATCTTGTGTCTGAGAACAGAATTTGCGTGACAATTCAGGAGATGCTGAACCTGGAAGGCATCGTATTGGAACCCG
CAGGCGCCCTGTCGGTGACGGTTCTGGACGAATTGCGCGGGCAGATCGCGGGCAAGACGGTGGTGTGTGTAACCTCTGGC
GGAAACTTTGATTTCGAACGGTTGCCAGAGGTGAAGGAGCGGGCCCAGAAGTGGCAGGGACTGAAAAAGTATTTCATCCT
GCGCCTGCCCCAACGGCCCGGAGCCTTGCGCGATTTCCTCGACCTCCTTGGGGAGGACGACAACATCACGCGGTTCGAGT
ATCTCAAGAAGAACTCTCGCAACTTCGGATCGGTGCTGATCGGGATCGAATCCCATCGTGCCGCGAGCTTCCCAGAGCTG
GAAGACCGCGTCGCCTCTCGCGGGTTTGGTTTCCGCGATATCACCGATGACCAAATTCTTGCGGATTTCCTGATCTAG

Upstream 100 bases:

>100_bases
CGCTTGTGCTGATATGGATCCAATTGCTGGTGAACCTGGCGCTGATGACCACGCTTTACGGCAATCTGATCGAAGGACGT
CAGTTGAACTGAGGCTGGAC

Downstream 100 bases:

>100_bases
GCGGCCGCCAGTTCCGGGACATGCGGGGCGACTTCGCTCAGCGCGGCGGCGAACGCGGTGCGCACAGCCTCCACATCCGG
GGGTTGGCTGGTATTGCAGG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTAGTGQAVGMTDFAIAAKATTAALRDLFPATPLLKNAYLSERFGADIWLKREDLSPVRSYKLRGAFNAMRKVRAERPEL
VDFVCASAGNHAQGVAYVCAHFGVRGRVYMPVTTPDQKILKTRTFGGDAVEIVLEGDFFDETQAAAKAYCAEVGAHFLSP
FDDPDVIEGQASVAVEMLEQLGRAPDHLVLPVGGGGLSSGMLSYLRDVGADTHATLVEPDGGPSLTAALKIGAPQTIEIT
DTFVDGAAVARIGNRNFEVLRNVPPRDVHLVSENRICVTIQEMLNLEGIVLEPAGALSVTVLDELRGQIAGKTVVCVTSG
GNFDFERLPEVKERAQKWQGLKKYFILRLPQRPGALRDFLDLLGEDDNITRFEYLKKNSRNFGSVLIGIESHRAASFPEL
EDRVASRGFGFRDITDDQILADFLI

Sequences:

>Translated_425_residues
MTAGTGQAVGMTDFAIAAKATTAALRDLFPATPLLKNAYLSERFGADIWLKREDLSPVRSYKLRGAFNAMRKVRAERPEL
VDFVCASAGNHAQGVAYVCAHFGVRGRVYMPVTTPDQKILKTRTFGGDAVEIVLEGDFFDETQAAAKAYCAEVGAHFLSP
FDDPDVIEGQASVAVEMLEQLGRAPDHLVLPVGGGGLSSGMLSYLRDVGADTHATLVEPDGGPSLTAALKIGAPQTIEIT
DTFVDGAAVARIGNRNFEVLRNVPPRDVHLVSENRICVTIQEMLNLEGIVLEPAGALSVTVLDELRGQIAGKTVVCVTSG
GNFDFERLPEVKERAQKWQGLKKYFILRLPQRPGALRDFLDLLGEDDNITRFEYLKKNSRNFGSVLIGIESHRAASFPEL
EDRVASRGFGFRDITDDQILADFLI
>Mature_424_residues
TAGTGQAVGMTDFAIAAKATTAALRDLFPATPLLKNAYLSERFGADIWLKREDLSPVRSYKLRGAFNAMRKVRAERPELV
DFVCASAGNHAQGVAYVCAHFGVRGRVYMPVTTPDQKILKTRTFGGDAVEIVLEGDFFDETQAAAKAYCAEVGAHFLSPF
DDPDVIEGQASVAVEMLEQLGRAPDHLVLPVGGGGLSSGMLSYLRDVGADTHATLVEPDGGPSLTAALKIGAPQTIEITD
TFVDGAAVARIGNRNFEVLRNVPPRDVHLVSENRICVTIQEMLNLEGIVLEPAGALSVTVLDELRGQIAGKTVVCVTSGG
NFDFERLPEVKERAQKWQGLKKYFILRLPQRPGALRDFLDLLGEDDNITRFEYLKKNSRNFGSVLIGIESHRAASFPELE
DRVASRGFGFRDITDDQILADFLI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=310, Percent_Identity=27.4193548387097, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33469958, Length=315, Percent_Identity=29.5238095238095, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1790207, Length=391, Percent_Identity=32.9923273657289, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789505, Length=303, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71991565, Length=336, Percent_Identity=29.1666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17537387, Length=343, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508781, Length=285, Percent_Identity=28.0701754385965, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320930, Length=415, Percent_Identity=31.566265060241, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322631, Length=304, Percent_Identity=30.2631578947368, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319788, Length=287, Percent_Identity=26.8292682926829, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355833, Length=337, Percent_Identity=30.5637982195846, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24645328, Length=259, Percent_Identity=30.5019305019305, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46213; Mature: 46082

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGTGQAVGMTDFAIAAKATTAALRDLFPATPLLKNAYLSERFGADIWLKREDLSPVRS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHH
YKLRGAFNAMRKVRAERPELVDFVCASAGNHAQGVAYVCAHFGVRGRVYMPVTTPDQKIL
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHH
KTRTFGGDAVEIVLEGDFFDETQAAAKAYCAEVGAHFLSPFDDPDVIEGQASVAVEMLEQ
HEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
LGRAPDHLVLPVGGGGLSSGMLSYLRDVGADTHATLVEPDGGPSLTAALKIGAPQTIEIT
HCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEE
DTFVDGAAVARIGNRNFEVLRNVPPRDVHLVSENRICVTIQEMLNLEGIVLEPAGALSVT
EHHHCCHHHHHHCCCCHHHHCCCCCCCEEEECCCCEEEEHHHHHCCCCEEECCCCCEEEH
VLDELRGQIAGKTVVCVTSGGNFDFERLPEVKERAQKWQGLKKYFILRLPQRPGALRDFL
HHHHHHHHHCCCEEEEEECCCCCCHHHCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
DLLGEDDNITRFEYLKKNSRNFGSVLIGIESHRAASFPELEDRVASRGFGFRDITDDQIL
HHHCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
ADFLI
HHHCC
>Mature Secondary Structure 
TAGTGQAVGMTDFAIAAKATTAALRDLFPATPLLKNAYLSERFGADIWLKREDLSPVRS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHH
YKLRGAFNAMRKVRAERPELVDFVCASAGNHAQGVAYVCAHFGVRGRVYMPVTTPDQKIL
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHH
KTRTFGGDAVEIVLEGDFFDETQAAAKAYCAEVGAHFLSPFDDPDVIEGQASVAVEMLEQ
HEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
LGRAPDHLVLPVGGGGLSSGMLSYLRDVGADTHATLVEPDGGPSLTAALKIGAPQTIEIT
HCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEE
DTFVDGAAVARIGNRNFEVLRNVPPRDVHLVSENRICVTIQEMLNLEGIVLEPAGALSVT
EHHHCCHHHHHHCCCCHHHHCCCCCCCEEEECCCCEEEEHHHHHCCCCEEECCCCCEEEH
VLDELRGQIAGKTVVCVTSGGNFDFERLPEVKERAQKWQGLKKYFILRLPQRPGALRDFL
HHHHHHHHHCCCEEEEEECCCCCCHHHCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
DLLGEDDNITRFEYLKKNSRNFGSVLIGIESHRAASFPELEDRVASRGFGFRDITDDQIL
HHHCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
ADFLI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]