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The map label for this gene is pbuE [H]

Identifier: 89052523

GI number: 89052523

Start: 29017

End: 30189

Strand: Reverse

Name: pbuE [H]

Synonym: Jann_0032

Alternate gene names: 89052523

Gene position: 30189-29017 (Counterclockwise)

Preceding gene: 89052524

Following gene: 89052522

Centisome position: 0.7

GC content: 65.22

Gene sequence:

>1173_bases
GTGACATCCCCCCCGGACGCCCCCGAGGGCTCCGTCAAACGTCTGATCCAGATCCTGTCGGTGACGAATTTCGTCATCGG
TATGGGGGCGTTTATGGTCATCGGTATGTTGCCTCCGATGGCCGAGAGCTTGGAGATGTCCACCGCCGGATCCGGCTGGA
TCATGACGATCTACGCGATCTCCTATGCCATCGCCTCGCCCTTGCTGGTGTCCAGCACCGGCGGGATCGGGCGGCGCAAG
GTGCTGTTCCTGGGTCTGGCCGTTTTCACGCTCGCCAACCTGCTTGCGGCAATCGCGCCGAGCGGAGCGGTGGTGCTGAT
CGCCCGTGCGTTCGCCGCGGCAGGGGCCGGTATGGTGACGCCGGTGGCGGCGGCTGTCGTGGCGGGCGTCGTGGCACCCG
AGCGGCGGGCGAAATCGCTGGCGGCGGTGTTTTTTGGGCTGACGTTGGCACAGGTGATCGGCGTGCCCGCGGGCAGCTAC
ATCGCCTATTCGATTGGTTGGCGCTACGCCTTCATCCTCGTCACGCTTCTGGCACTGCCCTGTCTCTGGCTGATCTGGAC
CCGCGTGCCCCGGGGCCTTCGGTTTCAACCGGTGGGCCTGCGCGATCTGAGCGCGGTCCTGGCCGATTGGCGCATCATGC
TGGCCGTTCTCTTTACCGCCACGTTCCTTGCCGCAATCTATGTGCCCTTCACCTATATTGCCCCGCTGATGGAGGCGCAT
ATGGGCTTCGGCCGTGACGGCATCAGCAGCGTATTGCTGACCTTCGGGCTCGCCGCTGTCGTTGGCAACCTTCTGGGCGG
TTGGATGTCGGATCGCTTTGGCCCCACACCCACGCTGATGCTGCTGGCCATCACGCAGGTGCTGCTGATGCCGTTTCTGT
CTTTTCTGCCGATGGCGGCCTGGATGGTATTCGCGCTGATCTTCACCTGGTCGATCTTCGGCTGGTCGTTCACCGCGCCG
CAACAGGCCCGTGTCATCAGCCTCGCGCCCGACCGTGCGCCCGTGGTTTTGTCCCTGAATGCGGCGGCGATCTATGTGGG
GGCTGCCGTGGGGGCGTCCGTCGGTGGGGCAGCGATCTCGCAATCCGGGCTGCTGGCGCTTGGCTGGGTTGGGGGCGCTA
TGGCGCTTGTGGGTGTGGCGCACCTGTTGGTGTCGGCCCGGATCAGCCGCTAG

Upstream 100 bases:

>100_bases
TGGGCCTATGCTCGGACGAGGTGCGTTTGCCGTTGGTGGGCGTCGACGAGTCCACCAAGGCCGCGATTGAGGCGGCACTG
CGCCACGCGGGCCTGCTGTC

Downstream 100 bases:

>100_bases
GCGTAGACACCTGTGGGCGGAGTGCCTAGATACACCGGACCATGGCCAAGAAACCTGATAATCCAAACTACAAAGTCATC
GCCGAGAACCGGCGGGCCCG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTSPPDAPEGSVKRLIQILSVTNFVIGMGAFMVIGMLPPMAESLEMSTAGSGWIMTIYAISYAIASPLLVSSTGGIGRRK
VLFLGLAVFTLANLLAAIAPSGAVVLIARAFAAAGAGMVTPVAAAVVAGVVAPERRAKSLAAVFFGLTLAQVIGVPAGSY
IAYSIGWRYAFILVTLLALPCLWLIWTRVPRGLRFQPVGLRDLSAVLADWRIMLAVLFTATFLAAIYVPFTYIAPLMEAH
MGFGRDGISSVLLTFGLAAVVGNLLGGWMSDRFGPTPTLMLLAITQVLLMPFLSFLPMAAWMVFALIFTWSIFGWSFTAP
QQARVISLAPDRAPVVLSLNAAAIYVGAAVGASVGGAAISQSGLLALGWVGGAMALVGVAHLLVSARISR

Sequences:

>Translated_390_residues
MTSPPDAPEGSVKRLIQILSVTNFVIGMGAFMVIGMLPPMAESLEMSTAGSGWIMTIYAISYAIASPLLVSSTGGIGRRK
VLFLGLAVFTLANLLAAIAPSGAVVLIARAFAAAGAGMVTPVAAAVVAGVVAPERRAKSLAAVFFGLTLAQVIGVPAGSY
IAYSIGWRYAFILVTLLALPCLWLIWTRVPRGLRFQPVGLRDLSAVLADWRIMLAVLFTATFLAAIYVPFTYIAPLMEAH
MGFGRDGISSVLLTFGLAAVVGNLLGGWMSDRFGPTPTLMLLAITQVLLMPFLSFLPMAAWMVFALIFTWSIFGWSFTAP
QQARVISLAPDRAPVVLSLNAAAIYVGAAVGASVGGAAISQSGLLALGWVGGAMALVGVAHLLVSARISR
>Mature_389_residues
TSPPDAPEGSVKRLIQILSVTNFVIGMGAFMVIGMLPPMAESLEMSTAGSGWIMTIYAISYAIASPLLVSSTGGIGRRKV
LFLGLAVFTLANLLAAIAPSGAVVLIARAFAAAGAGMVTPVAAAVVAGVVAPERRAKSLAAVFFGLTLAQVIGVPAGSYI
AYSIGWRYAFILVTLLALPCLWLIWTRVPRGLRFQPVGLRDLSAVLADWRIMLAVLFTATFLAAIYVPFTYIAPLMEAHM
GFGRDGISSVLLTFGLAAVVGNLLGGWMSDRFGPTPTLMLLAITQVLLMPFLSFLPMAAWMVFALIFTWSIFGWSFTAPQ
QARVISLAPDRAPVVLSLNAAAIYVGAAVGASVGGAAISQSGLLALGWVGGAMALVGVAHLLVSARISR

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=327, Percent_Identity=27.82874617737, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786595, Length=391, Percent_Identity=26.0869565217391, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI145693198, Length=367, Percent_Identity=27.7929155313352, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787808, Length=357, Percent_Identity=26.0504201680672, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40717; Mature: 40586

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSPPDAPEGSVKRLIQILSVTNFVIGMGAFMVIGMLPPMAESLEMSTAGSGWIMTIYAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHH
SYAIASPLLVSSTGGIGRRKVLFLGLAVFTLANLLAAIAPSGAVVLIARAFAAAGAGMVT
HHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
PVAAAVVAGVVAPERRAKSLAAVFFGLTLAQVIGVPAGSYIAYSIGWRYAFILVTLLALP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
CLWLIWTRVPRGLRFQPVGLRDLSAVLADWRIMLAVLFTATFLAAIYVPFTYIAPLMEAH
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFGRDGISSVLLTFGLAAVVGNLLGGWMSDRFGPTPTLMLLAITQVLLMPFLSFLPMAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WMVFALIFTWSIFGWSFTAPQQARVISLAPDRAPVVLSLNAAAIYVGAAVGASVGGAAIS
HHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHCCCCCHHHC
QSGLLALGWVGGAMALVGVAHLLVSARISR
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]