Click here to switch to the map view.

The map label for this gene is recF

Identifier: 89052494

GI number: 89052494

Start: 2749

End: 3876

Strand: Direct

Name: recF

Synonym: Jann_0003

Alternate gene names: 89052494

Gene position: 2749-3876 (Clockwise)

Preceding gene: 89052493

Following gene: 89052495

Centisome position: 0.06

GC content: 64.45

Gene sequence:

>1128_bases
ATGTCCAGGCAAAATGATGAGGGCTGTGGGGTGAGCAAAGTGTTCGTCTCATCCCTGGCGCTGTCGCATTTCCGCTCCCA
CAGACGGGCGCGAATGGAGTTGGACGGGCGCCCAGTGGCGCTGTTCGGACCCAATGGCGCGGGCAAGACCAATCTGATGG
AAGCTGTCTCCTTGCTGTCGCCCGGGCGTGGGTTGCGGCGCGCGGCAGCCGAAGAGATCATTCGCAGGCCTGAGGCGATT
GGCTGGAAAGTCAGCGCCGAGATTTCCGGGCCCAGCATTAACCACGATATCACTCTGACAGCCGAACCCGGACAACCCCG
CACCACTCAGGTCGACGGCAAGACCGCACCACAGATCGCGCTTGCCAGGCTGCTGCGCATTGTCTGGCTGGTGCCGTCTC
AAGACAGGCTCTGGAGCGAAGGGGCAGAAGGGCGGCGGCGGTTTCTTGACCGGATCACGCTAAGTTTTCTGCCCGACCAC
GCAGATGCCGTCCTGACCTACGAGAAAGCGATGCGCGAGAGGAACCGTCTGCTCCGCGACGATGCGCGTGATCCGGCGTG
GTATCGCGCGTTGGAGGCGCAGATGGCAGACGCCGCTGTGCGGATCGTGAACGGGCGCGACGACGCGCTCACCCGCATCA
GCGCTGCCCAGAATGGGGCTGCGACAGCTTTCCCCGCCGCCGATCTGGGGATCGAAACGGAGCACCCCTGCCAAACGGTC
GACGATTTCATCCAGGCCTTCGAGGGGAGCCGCCCACGGGACCTCGCAGCCGGGCGCACGCTGATCGGACCGCACCGCGC
CGACATGTCCGCGATCTACCGCGACAAGGGTGTGCCCGCCAAACAATGCTCCACCGGTGAGCAGAAAGCGCTGCTGATCT
CGCTGATCCTGTCCAACGCACGGGCCTTGAAGGCCGAGACCGGAACGGCCCCCTTGGTGCTGCTGGATGAGGTCGCGGCG
CATCTGGATGCGGGGCGTCGCGCAGCGCTGTTTGATGAGATCTGCGCGTTGGAGGCTCAGGCCTGGATGACCGGAACAGG
GCCGGAATTGTTCGCGGAATTGGGTGACAGGGCGCAGCATTTCGAGATTACCGAGGTCGCAGGCGAAAGCCAGGTCGCCG
CCCCCTAA

Upstream 100 bases:

>100_bases
GGCTGAGCCCACGCTGATGCGCGAAGGCAACGATGATAGCGCGATCTATGTCGTCATGCCCATGCGGGTCTGACCGGCGC
GCGCGTGACCTCCACCGGAC

Downstream 100 bases:

>100_bases
ATCTTGTGTTCCTCCCGTGACATTGCTTGCCAAAATGCGTATATATTGAGCAACAGAACGGGACAAAAAAGACATGAACG
ACATACCCGCGAGCGGTGAC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSRQNDEGCGVSKVFVSSLALSHFRSHRRARMELDGRPVALFGPNGAGKTNLMEAVSLLSPGRGLRRAAAEEIIRRPEAI
GWKVSAEISGPSINHDITLTAEPGQPRTTQVDGKTAPQIALARLLRIVWLVPSQDRLWSEGAEGRRRFLDRITLSFLPDH
ADAVLTYEKAMRERNRLLRDDARDPAWYRALEAQMADAAVRIVNGRDDALTRISAAQNGAATAFPAADLGIETEHPCQTV
DDFIQAFEGSRPRDLAAGRTLIGPHRADMSAIYRDKGVPAKQCSTGEQKALLISLILSNARALKAETGTAPLVLLDEVAA
HLDAGRRAALFDEICALEAQAWMTGTGPELFAELGDRAQHFEITEVAGESQVAAP

Sequences:

>Translated_375_residues
MSRQNDEGCGVSKVFVSSLALSHFRSHRRARMELDGRPVALFGPNGAGKTNLMEAVSLLSPGRGLRRAAAEEIIRRPEAI
GWKVSAEISGPSINHDITLTAEPGQPRTTQVDGKTAPQIALARLLRIVWLVPSQDRLWSEGAEGRRRFLDRITLSFLPDH
ADAVLTYEKAMRERNRLLRDDARDPAWYRALEAQMADAAVRIVNGRDDALTRISAAQNGAATAFPAADLGIETEHPCQTV
DDFIQAFEGSRPRDLAAGRTLIGPHRADMSAIYRDKGVPAKQCSTGEQKALLISLILSNARALKAETGTAPLVLLDEVAA
HLDAGRRAALFDEICALEAQAWMTGTGPELFAELGDRAQHFEITEVAGESQVAAP
>Mature_374_residues
SRQNDEGCGVSKVFVSSLALSHFRSHRRARMELDGRPVALFGPNGAGKTNLMEAVSLLSPGRGLRRAAAEEIIRRPEAIG
WKVSAEISGPSINHDITLTAEPGQPRTTQVDGKTAPQIALARLLRIVWLVPSQDRLWSEGAEGRRRFLDRITLSFLPDHA
DAVLTYEKAMRERNRLLRDDARDPAWYRALEAQMADAAVRIVNGRDDALTRISAAQNGAATAFPAADLGIETEHPCQTVD
DFIQAFEGSRPRDLAAGRTLIGPHRADMSAIYRDKGVPAKQCSTGEQKALLISLILSNARALKAETGTAPLVLLDEVAAH
LDAGRRAALFDEICALEAQAWMTGTGPELFAELGDRAQHFEITEVAGESQVAAP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=344, Percent_Identity=25.8720930232558, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40723; Mature: 40591

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQNDEGCGVSKVFVSSLALSHFRSHRRARMELDGRPVALFGPNGAGKTNLMEAVSLLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHC
PGRGLRRAAAEEIIRRPEAIGWKVSAEISGPSINHDITLTAEPGQPRTTQVDGKTAPQIA
CCCHHHHHHHHHHHHCCHHCCEEEEEEECCCCCCCCEEEEECCCCCCCEECCCCCCHHHH
LARLLRIVWLVPSQDRLWSEGAEGRRRFLDRITLSFLPDHADAVLTYEKAMRERNRLLRD
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
DARDPAWYRALEAQMADAAVRIVNGRDDALTRISAAQNGAATAFPAADLGIETEHPCQTV
CCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHH
DDFIQAFEGSRPRDLAAGRTLIGPHRADMSAIYRDKGVPAKQCSTGEQKALLISLILSNA
HHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCC
RALKAETGTAPLVLLDEVAAHLDAGRRAALFDEICALEAQAWMTGTGPELFAELGDRAQH
HHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHC
FEITEVAGESQVAAP
EEHHHHCCCCCCCCC
>Mature Secondary Structure 
SRQNDEGCGVSKVFVSSLALSHFRSHRRARMELDGRPVALFGPNGAGKTNLMEAVSLLS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHC
PGRGLRRAAAEEIIRRPEAIGWKVSAEISGPSINHDITLTAEPGQPRTTQVDGKTAPQIA
CCCHHHHHHHHHHHHCCHHCCEEEEEEECCCCCCCCEEEEECCCCCCCEECCCCCCHHHH
LARLLRIVWLVPSQDRLWSEGAEGRRRFLDRITLSFLPDHADAVLTYEKAMRERNRLLRD
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
DARDPAWYRALEAQMADAAVRIVNGRDDALTRISAAQNGAATAFPAADLGIETEHPCQTV
CCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHH
DDFIQAFEGSRPRDLAAGRTLIGPHRADMSAIYRDKGVPAKQCSTGEQKALLISLILSNA
HHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCC
RALKAETGTAPLVLLDEVAAHLDAGRRAALFDEICALEAQAWMTGTGPELFAELGDRAQH
HHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHC
FEITEVAGESQVAAP
EEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA