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The map label for this gene is dnaN [H]

Identifier: 89052493

GI number: 89052493

Start: 1603

End: 2721

Strand: Direct

Name: dnaN [H]

Synonym: Jann_0002

Alternate gene names: 89052493

Gene position: 1603-2721 (Clockwise)

Preceding gene: 89052492

Following gene: 89052494

Centisome position: 0.04

GC content: 60.95

Gene sequence:

>1119_bases
ATGAAACTCTCCATCGAACGCGCCACATTGCTGCGCGCTGTCAGCCAGGCGCAATCCGTGGTGGAGCGTCGCAACACCAT
CCCGATCCTCGCCAACGTATTGATTGAGGCCGAGGGAGAGACTGTCAGCTTCCGCGCCACCGATCTGGACATTGAAGTCG
TCGACAAGGCCCCCGCCCAGGTGGAGCGTGCCGGGGCCACGACCGTGTCCGCCGTGACATTGCACGAGATCGTGCGCAAA
TTGCCCGACGGATCGCTGGTGTCACTGACGGAAGATACCACCACGTCGCGACTTACGATTGAAGCGGGACGGTCCTCCTT
TCAACTGGCGACGCTGCCGCGCGAAGATTTTCCCGTCATGGCGTCAACGGATTATGCCGCCAATTTCTCGGCCAAAGCGC
CGGAGCTGCGACGCCTGTTCGACAAGTCCAAGTTTGCGATCTCGACCGAAGAGACGCGGTATTATCTGAACGGTGTCTAT
TTCCACATCGCTGAAGGCGATAACGGCAAAGCCCTGCGTGCCGTGGCCACCGACGGCCACCGCCTTGCCCGGATCGACAG
CGCCCTGCCCGATGGTGCGCAAGAAATGCCCGGCGTGATCGTGCCTCGCAAGACGGTGGGCGAACTGCGCAAGCTGCTGG
ATGATGACGATGCCCAGATCGCCGTGAGCGTGTCGGAGACGAAAATCCGCTTCGCCACGCCAGATATCACGCTGACCTCC
AAGGTGATCGACGGCACCTTCCCCGATTACACACGCGTCATTCCAGCGGGCAACACGCGCCGGATGGAAGTGGATGCGGG
CGACTTCGCCAAGGCGGTCGATCTGGTGGCAACGGTCAGCTCCGAGCGGTCACGCGCCGTAAAGATGGCGCTGGACGAGG
ACCGCCTGATGCTGTCCGTTAATGCGCCAGATTCTGGCAATGCAGAGGCCGAACTGGTCGTGGCCTATGCCGACGAGAAG
CTGGAGATTGGCTTTAACGCCAAATACTTGCTGGAAATCGCCAGCCAGGTGGACCGGGAGAACGCGGTTTTCATGTTCTC
GTCCCCGGCTGAGCCCACGCTGATGCGCGAAGGCAACGATGATAGCGCGATCTATGTCGTCATGCCCATGCGGGTCTGA

Upstream 100 bases:

>100_bases
ACTTGACGCTGGCAAGCGCGCAGGCAGTATCTTATTCAACTTGTGAATTTCTGGGGTCCGAGTAGTCTCGCGGCCCCACA
CTCTGTCGGAGCGCACGTCC

Downstream 100 bases:

>100_bases
CCGGCGCGCGCGTGACCTCCACCGGACATGTCCAGGCAAAATGATGAGGGCTGTGGGGTGAGCAAAGTGTTCGTCTCATC
CCTGGCGCTGTCGCATTTCC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKLSIERATLLRAVSQAQSVVERRNTIPILANVLIEAEGETVSFRATDLDIEVVDKAPAQVERAGATTVSAVTLHEIVRK
LPDGSLVSLTEDTTTSRLTIEAGRSSFQLATLPREDFPVMASTDYAANFSAKAPELRRLFDKSKFAISTEETRYYLNGVY
FHIAEGDNGKALRAVATDGHRLARIDSALPDGAQEMPGVIVPRKTVGELRKLLDDDDAQIAVSVSETKIRFATPDITLTS
KVIDGTFPDYTRVIPAGNTRRMEVDAGDFAKAVDLVATVSSERSRAVKMALDEDRLMLSVNAPDSGNAEAELVVAYADEK
LEIGFNAKYLLEIASQVDRENAVFMFSSPAEPTLMREGNDDSAIYVVMPMRV

Sequences:

>Translated_372_residues
MKLSIERATLLRAVSQAQSVVERRNTIPILANVLIEAEGETVSFRATDLDIEVVDKAPAQVERAGATTVSAVTLHEIVRK
LPDGSLVSLTEDTTTSRLTIEAGRSSFQLATLPREDFPVMASTDYAANFSAKAPELRRLFDKSKFAISTEETRYYLNGVY
FHIAEGDNGKALRAVATDGHRLARIDSALPDGAQEMPGVIVPRKTVGELRKLLDDDDAQIAVSVSETKIRFATPDITLTS
KVIDGTFPDYTRVIPAGNTRRMEVDAGDFAKAVDLVATVSSERSRAVKMALDEDRLMLSVNAPDSGNAEAELVVAYADEK
LEIGFNAKYLLEIASQVDRENAVFMFSSPAEPTLMREGNDDSAIYVVMPMRV
>Mature_372_residues
MKLSIERATLLRAVSQAQSVVERRNTIPILANVLIEAEGETVSFRATDLDIEVVDKAPAQVERAGATTVSAVTLHEIVRK
LPDGSLVSLTEDTTTSRLTIEAGRSSFQLATLPREDFPVMASTDYAANFSAKAPELRRLFDKSKFAISTEETRYYLNGVY
FHIAEGDNGKALRAVATDGHRLARIDSALPDGAQEMPGVIVPRKTVGELRKLLDDDDAQIAVSVSETKIRFATPDITLTS
KVIDGTFPDYTRVIPAGNTRRMEVDAGDFAKAVDLVATVSSERSRAVKMALDEDRLMLSVNAPDSGNAEAELVVAYADEK
LEIGFNAKYLLEIASQVDRENAVFMFSSPAEPTLMREGNDDSAIYVVMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=36.2666666666667, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40605; Mature: 40605

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSIERATLLRAVSQAQSVVERRNTIPILANVLIEAEGETVSFRATDLDIEVVDKAPAQ
CCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEECCEEEEECCCCHH
VERAGATTVSAVTLHEIVRKLPDGSLVSLTEDTTTSRLTIEAGRSSFQLATLPREDFPVM
HHHCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCEEEEECCCCCCCEE
ASTDYAANFSAKAPELRRLFDKSKFAISTEETRYYLNGVYFHIAEGDNGKALRAVATDGH
EECCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEECCCCCEEEEEECCCC
RLARIDSALPDGAQEMPGVIVPRKTVGELRKLLDDDDAQIAVSVSETKIRFATPDITLTS
EEEEHHCCCCCCHHHCCCEEECHHHHHHHHHHHCCCCCEEEEEEECEEEEEECCCEEEEH
KVIDGTFPDYTRVIPAGNTRRMEVDAGDFAKAVDLVATVSSERSRAVKMALDEDRLMLSV
HHHCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCHHHEEEEEECCCEEEEEE
NAPDSGNAEAELVVAYADEKLEIGFNAKYLLEIASQVDRENAVFMFSSPAEPTLMREGND
ECCCCCCCCEEEEEEEECCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCC
DSAIYVVMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MKLSIERATLLRAVSQAQSVVERRNTIPILANVLIEAEGETVSFRATDLDIEVVDKAPAQ
CCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEECCEEEEECCCCHH
VERAGATTVSAVTLHEIVRKLPDGSLVSLTEDTTTSRLTIEAGRSSFQLATLPREDFPVM
HHHCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCEEEEECCCCCCCEE
ASTDYAANFSAKAPELRRLFDKSKFAISTEETRYYLNGVYFHIAEGDNGKALRAVATDGH
EECCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEECCCCCEEEEEECCCC
RLARIDSALPDGAQEMPGVIVPRKTVGELRKLLDDDDAQIAVSVSETKIRFATPDITLTS
EEEEHHCCCCCCHHHCCCEEECHHHHHHHHHHHCCCCCEEEEEEECEEEEEECCCEEEEH
KVIDGTFPDYTRVIPAGNTRRMEVDAGDFAKAVDLVATVSSERSRAVKMALDEDRLMLSV
HHHCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCHHHEEEEEECCCEEEEEE
NAPDSGNAEAELVVAYADEKLEIGFNAKYLLEIASQVDRENAVFMFSSPAEPTLMREGND
ECCCCCCCCEEEEEEEECCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCC
DSAIYVVMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]