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The map label for this gene is smpB

Identifier: 88658487

GI number: 88658487

Start: 123530

End: 123979

Strand: Direct

Name: smpB

Synonym: ECH_0135

Alternate gene names: 88658487

Gene position: 123530-123979 (Clockwise)

Preceding gene: 88658144

Following gene: 88657620

Centisome position: 10.5

GC content: 32.22

Gene sequence:

>450_bases
GTGGATATCGTAGTTGAGAATCGTAAAGTAAGGTTTAATTATTTTATTATTCAGGAATTTGATGCTGGTATTGTATTAAT
TGGTAGTGAAGTCAAGTCTTTACGCCAGAGAAAAGTCAGCATAGCTGAGGCATATGTTACTGAGCGGAATATGGAATTGT
GGCTATGTAATTTACATATTTCAGAATATAATCAGGCAAGTACTAGAAATCATAATCCTACCAGACCTAGGAAGTTACTA
TTGCGGAAAAAGCAAATATATAAAATATCTGGAAATATAAAGAATGATGGCTTTACTGTTGTACCATTGTGTTTATATTT
TAATGATAAGGGGATAGCAAAAGTTAAGGTTGTTATTGTAAAAGGTAAAAAGTTGCATGATAAGAGAGAGACAATAAAAA
CTAGAGATTGGAATAGAGAAAAAAGTCGTATACTACGTGGCGGAAACTAA

Upstream 100 bases:

>100_bases
ATAGAGTTTTCCCAGCGTTATAACATTAAAGTTACTAGTATAGATAAACTTATTCAATATGTAAAAGCAAACTAGTGTAT
GTTGGGAGTGCAATTTTTCT

Downstream 100 bases:

>100_bases
TCTGATATAGAGAAAATAGTTTATAGACTTAACTTTGTTGTGATACAGAGTTTGGTTTAAAGTAGATATATCAATACATA
TTTATTGTAAAGCATTCTCC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MDIVVENRKVRFNYFIIQEFDAGIVLIGSEVKSLRQRKVSIAEAYVTERNMELWLCNLHISEYNQASTRNHNPTRPRKLL
LRKKQIYKISGNIKNDGFTVVPLCLYFNDKGIAKVKVVIVKGKKLHDKRETIKTRDWNREKSRILRGGN

Sequences:

>Translated_149_residues
MDIVVENRKVRFNYFIIQEFDAGIVLIGSEVKSLRQRKVSIAEAYVTERNMELWLCNLHISEYNQASTRNHNPTRPRKLL
LRKKQIYKISGNIKNDGFTVVPLCLYFNDKGIAKVKVVIVKGKKLHDKRETIKTRDWNREKSRILRGGN
>Mature_149_residues
MDIVVENRKVRFNYFIIQEFDAGIVLIGSEVKSLRQRKVSIAEAYVTERNMELWLCNLHISEYNQASTRNHNPTRPRKLL
LRKKQIYKISGNIKNDGFTVVPLCLYFNDKGIAKVKVVIVKGKKLHDKRETIKTRDWNREKSRILRGGN

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=43.0555555555556, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_EHRCR (Q2GHX0)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_506963.1
- ProteinModelPortal:   Q2GHX0
- SMR:   Q2GHX0
- STRING:   Q2GHX0
- GeneID:   3928033
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0135
- TIGR:   ECH_0135
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   ECHA205920:ECH_0135-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17473; Mature: 17473

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVVENRKVRFNYFIIQEFDAGIVLIGSEVKSLRQRKVSIAEAYVTERNMELWLCNLHI
CCEEECCCEEEEEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
SEYNQASTRNHNPTRPRKLLLRKKQIYKISGNIKNDGFTVVPLCLYFNDKGIAKVKVVIV
CCCCCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEEEE
KGKKLHDKRETIKTRDWNREKSRILRGGN
ECCCCCCHHHHHCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MDIVVENRKVRFNYFIIQEFDAGIVLIGSEVKSLRQRKVSIAEAYVTERNMELWLCNLHI
CCEEECCCEEEEEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
SEYNQASTRNHNPTRPRKLLLRKKQIYKISGNIKNDGFTVVPLCLYFNDKGIAKVKVVIV
CCCCCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEEEE
KGKKLHDKRETIKTRDWNREKSRILRGGN
ECCCCCCHHHHHCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA