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The map label for this gene is ribB [H]

Identifier: 88658144

GI number: 88658144

Start: 122869

End: 123504

Strand: Direct

Name: ribB [H]

Synonym: ECH_0134

Alternate gene names: 88658144

Gene position: 122869-123504 (Clockwise)

Preceding gene: 88657640

Following gene: 88658487

Centisome position: 10.45

GC content: 33.65

Gene sequence:

>636_bases
ATGGGTATTATTGTATCAGAAAATTCGAGTTTTATCTCTCCTGTAGAGCATATTATTGAAGATTCTAAACAAGGTAAAAT
GTTTATTTTGATTGATGATGAACAAAGGGAAAATGAAGGTGATTTGGTAGTTTTAGCAGAAAAGGTAACAAGTGGTGTAA
TTAACTTTATGATAACTCATGGTAGTGGTATTGTGTGTTTATCTATTACAGAAGATTTAATGCATAAATTGGGGTTGGAG
TTTATACCAAAGAGGCATGTTAATTGTAATAGTGCGTCTTTTGCAACTTCTATAGATGCTCGTTATGGTATTACAACTGG
TGTTTCTGCTCATGATAGAGCCCATACTATACTTACAGTTGTTAATAAAAATGTTACAGAGGATGATTTTGTAACACCGG
GGCATATTTTTCCTATTGTATCTAAAAAAGGTGGAGTGTTAGAGAGATCTGGTCATACAGAAGCGAGTGTTGAAATTGCA
AGAATTGCAGGGTTAGCTCCAGTAGCTGTAGTATGTGAGTTGATGAATCCTGATGGAACAATGTCAAGGATGCCTGATAT
TATAGAGTTTTCCCAGCGTTATAACATTAAAGTTACTAGTATAGATAAACTTATTCAATATGTAAAAGCAAACTAG

Upstream 100 bases:

>100_bases
AAAAGAAGGTGATGTTGTAGAAGTTAAAACTCCAAAGGGTGATTATAGAATATATAAAATTATAAAAATTGAATTTATAT
AAAAGAAGGTTATAGGGTTA

Downstream 100 bases:

>100_bases
TGTATGTTGGGAGTGCAATTTTTCTGTGGATATCGTAGTTGAGAATCGTAAAGTAAGGTTTAATTATTTTATTATTCAGG
AATTTGATGCTGGTATTGTA

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MGIIVSENSSFISPVEHIIEDSKQGKMFILIDDEQRENEGDLVVLAEKVTSGVINFMITHGSGIVCLSITEDLMHKLGLE
FIPKRHVNCNSASFATSIDARYGITTGVSAHDRAHTILTVVNKNVTEDDFVTPGHIFPIVSKKGGVLERSGHTEASVEIA
RIAGLAPVAVVCELMNPDGTMSRMPDIIEFSQRYNIKVTSIDKLIQYVKAN

Sequences:

>Translated_211_residues
MGIIVSENSSFISPVEHIIEDSKQGKMFILIDDEQRENEGDLVVLAEKVTSGVINFMITHGSGIVCLSITEDLMHKLGLE
FIPKRHVNCNSASFATSIDARYGITTGVSAHDRAHTILTVVNKNVTEDDFVTPGHIFPIVSKKGGVLERSGHTEASVEIA
RIAGLAPVAVVCELMNPDGTMSRMPDIIEFSQRYNIKVTSIDKLIQYVKAN
>Mature_210_residues
GIIVSENSSFISPVEHIIEDSKQGKMFILIDDEQRENEGDLVVLAEKVTSGVINFMITHGSGIVCLSITEDLMHKLGLEF
IPKRHVNCNSASFATSIDARYGITTGVSAHDRAHTILTVVNKNVTEDDFVTPGHIFPIVSKKGGVLERSGHTEASVEIAR
IAGLAPVAVVCELMNPDGTMSRMPDIIEFSQRYNIKVTSIDKLIQYVKAN

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=206, Percent_Identity=41.2621359223301, Blast_Score=179, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320695, Length=211, Percent_Identity=39.3364928909953, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 23067; Mature: 22936

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIIVSENSSFISPVEHIIEDSKQGKMFILIDDEQRENEGDLVVLAEKVTSGVINFMITH
CCEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHEEEEEEEC
GSGIVCLSITEDLMHKLGLEFIPKRHVNCNSASFATSIDARYGITTGVSAHDRAHTILTV
CCCEEEEEEHHHHHHHCCCHHCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCCEEEEEE
VNKNVTEDDFVTPGHIFPIVSKKGGVLERSGHTEASVEIARIAGLAPVAVVCELMNPDGT
ECCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCHHEEHHHHHCCCHHHHHHHHCCCCCC
MSRMPDIIEFSQRYNIKVTSIDKLIQYVKAN
HHHCCHHHHHHHHCCEEEECHHHHHHHHHCC
>Mature Secondary Structure 
GIIVSENSSFISPVEHIIEDSKQGKMFILIDDEQRENEGDLVVLAEKVTSGVINFMITH
CEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHEEEEEEEC
GSGIVCLSITEDLMHKLGLEFIPKRHVNCNSASFATSIDARYGITTGVSAHDRAHTILTV
CCCEEEEEEHHHHHHHCCCHHCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCCEEEEEE
VNKNVTEDDFVTPGHIFPIVSKKGGVLERSGHTEASVEIARIAGLAPVAVVCELMNPDGT
ECCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCHHEEHHHHHCCCHHHHHHHHCCCCCC
MSRMPDIIEFSQRYNIKVTSIDKLIQYVKAN
HHHCCHHHHHHHHCCEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]