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The map label for this gene is greA [H]

Identifier: 88657640

GI number: 88657640

Start: 122362

End: 122850

Strand: Direct

Name: greA [H]

Synonym: ECH_0133

Alternate gene names: 88657640

Gene position: 122362-122850 (Clockwise)

Preceding gene: 88657691

Following gene: 88658144

Centisome position: 10.4

GC content: 31.9

Gene sequence:

>489_bases
ATGAATGCGAGATTTCCTATTACAAAAAATGGGTTTGCAAAACTTGAGTTAGAGCTAGAGTCATTGAAACATGAAAGGCC
TAAAATTATAAAATCTATTTCTGAGGCGCGAGAGTTGGGGGATTTATCTGAAAATGCAGAGTATCATGCTGCTCGTGAAA
GACAAGGGTTAGTAGAAAGTAAAATAATGGAGTTAGAATCGAAAAAATCTAGAGCAGAAGTTATAGATGTTTCAAATTTA
TCTGGGGACACTGTTATGTTTGGGGCAACTGTAACTATGTCTATATATGATGAACAGACTGGTAATACTGATGAAGTAGT
GTACCAGATTGTTGGTGAGTATGAAGCTGATATTTCTAAACAAATGATTTCAATAAAATCTCCTTTAGTTGTATCATTAT
TAGGGAAAAAAGAAGGTGATGTTGTAGAAGTTAAAACTCCAAAGGGTGATTATAGAATATATAAAATTATAAAAATTGAA
TTTATATAA

Upstream 100 bases:

>100_bases
TACTGATGATGTAAAAGACTTATTATTAGAAATTATAGGTAAGGTTACTTCTAATTTTGAATAGTTCTGTATTATGTAGG
TATGACTTATGAATGACTAT

Downstream 100 bases:

>100_bases
AAGAAGGTTATAGGGTTAATGGGTATTATTGTATCAGAAAATTCGAGTTTTATCTCTCCTGTAGAGCATATTATTGAAGA
TTCTAAACAAGGTAAAATGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNARFPITKNGFAKLELELESLKHERPKIIKSISEARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVIDVSNL
SGDTVMFGATVTMSIYDEQTGNTDEVVYQIVGEYEADISKQMISIKSPLVVSLLGKKEGDVVEVKTPKGDYRIYKIIKIE
FI

Sequences:

>Translated_162_residues
MNARFPITKNGFAKLELELESLKHERPKIIKSISEARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVIDVSNL
SGDTVMFGATVTMSIYDEQTGNTDEVVYQIVGEYEADISKQMISIKSPLVVSLLGKKEGDVVEVKTPKGDYRIYKIIKIE
FI
>Mature_162_residues
MNARFPITKNGFAKLELELESLKHERPKIIKSISEARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVIDVSNL
SGDTVMFGATVTMSIYDEQTGNTDEVVYQIVGEYEADISKQMISIKSPLVVSLLGKKEGDVVEVKTPKGDYRIYKIIKIE
FI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=160, Percent_Identity=48.125, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=33.9622641509434, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18229; Mature: 18229

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARFPITKNGFAKLELELESLKHERPKIIKSISEARELGDLSENAEYHAARERQGLVES
CCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHH
KIMELESKKSRAEVIDVSNLSGDTVMFGATVTMSIYDEQTGNTDEVVYQIVGEYEADISK
HHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QMISIKSPLVVSLLGKKEGDVVEVKTPKGDYRIYKIIKIEFI
HHHHHCCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MNARFPITKNGFAKLELELESLKHERPKIIKSISEARELGDLSENAEYHAARERQGLVES
CCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHH
KIMELESKKSRAEVIDVSNLSGDTVMFGATVTMSIYDEQTGNTDEVVYQIVGEYEADISK
HHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QMISIKSPLVVSLLGKKEGDVVEVKTPKGDYRIYKIIKIEFI
HHHHHCCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA