Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 88658073

GI number: 88658073

Start: 543059

End: 543784

Strand: Direct

Name: rsmE [H]

Synonym: ECH_0540

Alternate gene names: 88658073

Gene position: 543059-543784 (Clockwise)

Preceding gene: 88658558

Following gene: 88657993

Centisome position: 46.17

GC content: 31.68

Gene sequence:

>726_bases
ATGATACAAAAGGCAAAAAAGCTGAATGTTAGGTTGTATGTGGATTGTACTCTTAGTAAATCTTCTATAGTAATATTAGA
TAATATTGCTTCTCACTATATATGTCATGTAATGCGTGCTGCGAAATTAGATGAGGTAAAGTTATTTAATGGCAGGGATG
GAGAATGGATAGGCGAAATATTTGAATTATCTAGCAATACTAAAATTAGAGTAAATGAGTTAATTAGAAGGCAATATGTA
CCTAAAAGTTTAACATTGTGTCTTTCTATTGTCAAAAATACTGCATTACAGAATGTCATTAGGCAAGCAACAGAAATGGG
AGTAACATTTATACAGCCTATTTATACACAACATATAGCAACAAGTAATATAAATTTATCTAAATGCAAAAAATGGGCTA
TTGAAGCTGCTGAACAATGTGGAAGGCTTGATATACCAGATATTTCTTTTCCTATTGATTTTATTGATTTAAAGCAGTTG
AAAACTGATGACAACAATTTCGTGATTTGTGATGAAACAGGTAAAGGTCATCCTCCATCAGAGGTTTTAAAAGGTAAGAA
TAACGTTCATATTATTATAGGGCCAGAAGGTGGTTTTTCAAATGATGAGTTAAGCTTTGCCTATAGTTTTTGTGATGGGT
TATCTTTAGGTACAACAATACTGAGAGTTGATACAGCAGTTGTTAGTGCATTAGCTTATGTAAAAGAGTATTATGGTATG
TTGTAG

Upstream 100 bases:

>100_bases
AAAATGGAAAAATAAGAAAAATGTGGAGAAATGTAAAAGTTAGTGGACATGTTGATAATGTTCTTGAGATAATAAGAGAG
ATAATGTAAATTGCAAACTA

Downstream 100 bases:

>100_bases
TAGAATTATGGTTGTTGAAGACATTGTCAATAAGAAAGCTGAGTTGCGAAAAAAATATAGAAAGTTAAGGAAAGAAGTAA
AAAATAAAAAAGAAGCGTCT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIQKAKKLNVRLYVDCTLSKSSIVILDNIASHYICHVMRAAKLDEVKLFNGRDGEWIGEIFELSSNTKIRVNELIRRQYV
PKSLTLCLSIVKNTALQNVIRQATEMGVTFIQPIYTQHIATSNINLSKCKKWAIEAAEQCGRLDIPDISFPIDFIDLKQL
KTDDNNFVICDETGKGHPPSEVLKGKNNVHIIIGPEGGFSNDELSFAYSFCDGLSLGTTILRVDTAVVSALAYVKEYYGM
L

Sequences:

>Translated_241_residues
MIQKAKKLNVRLYVDCTLSKSSIVILDNIASHYICHVMRAAKLDEVKLFNGRDGEWIGEIFELSSNTKIRVNELIRRQYV
PKSLTLCLSIVKNTALQNVIRQATEMGVTFIQPIYTQHIATSNINLSKCKKWAIEAAEQCGRLDIPDISFPIDFIDLKQL
KTDDNNFVICDETGKGHPPSEVLKGKNNVHIIIGPEGGFSNDELSFAYSFCDGLSLGTTILRVDTAVVSALAYVKEYYGM
L
>Mature_241_residues
MIQKAKKLNVRLYVDCTLSKSSIVILDNIASHYICHVMRAAKLDEVKLFNGRDGEWIGEIFELSSNTKIRVNELIRRQYV
PKSLTLCLSIVKNTALQNVIRQATEMGVTFIQPIYTQHIATSNINLSKCKKWAIEAAEQCGRLDIPDISFPIDFIDLKQL
KTDDNNFVICDETGKGHPPSEVLKGKNNVHIIIGPEGGFSNDELSFAYSFCDGLSLGTTILRVDTAVVSALAYVKEYYGM
L

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=255, Percent_Identity=27.0588235294118, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26964; Mature: 26964

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKAKKLNVRLYVDCTLSKSSIVILDNIASHYICHVMRAAKLDEVKLFNGRDGEWIGEI
CCCCCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
FELSSNTKIRVNELIRRQYVPKSLTLCLSIVKNTALQNVIRQATEMGVTFIQPIYTQHIA
HHCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TSNINLSKCKKWAIEAAEQCGRLDIPDISFPIDFIDLKQLKTDDNNFVICDETGKGHPPS
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHH
EVLKGKNNVHIIIGPEGGFSNDELSFAYSFCDGLSLGTTILRVDTAVVSALAYVKEYYGM
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
L
C
>Mature Secondary Structure
MIQKAKKLNVRLYVDCTLSKSSIVILDNIASHYICHVMRAAKLDEVKLFNGRDGEWIGEI
CCCCCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
FELSSNTKIRVNELIRRQYVPKSLTLCLSIVKNTALQNVIRQATEMGVTFIQPIYTQHIA
HHCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TSNINLSKCKKWAIEAAEQCGRLDIPDISFPIDFIDLKQLKTDDNNFVICDETGKGHPPS
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHH
EVLKGKNNVHIIIGPEGGFSNDELSFAYSFCDGLSLGTTILRVDTAVVSALAYVKEYYGM
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]