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The map label for this gene is glnG [C]

Identifier: 88657684

GI number: 88657684

Start: 328496

End: 329893

Strand: Direct

Name: glnG [C]

Synonym: ECH_0339

Alternate gene names: 88657684

Gene position: 328496-329893 (Clockwise)

Preceding gene: 88658432

Following gene: 88658398

Centisome position: 27.93

GC content: 33.19

Gene sequence:

>1398_bases
ATGTCCAAAGAAAGATTGTATATTTCTGAAGTATTGGTTGTTGATGATGAAGTTGATATTAGGAATTTAATAAAGGATAT
ATTAAGTGATGATAATTATGTCACAAAATTAGCAGTTGATGGGTTGTCTGCTATTAAGATGGCTTATGAGAAAGAACCAG
ATGTTGTTTTACTAGATATATGGTTAAAAGGATCAGATATTGATGGGTTAAGTGTTTTAGAGAAACTAAAGGAAAGGTAT
CCATATTTACCTGTGATTATGATTAGTGGACATGGTAATATTGCTACTGCTGTGAAGTCTTTGCACATGGGAGCTTATGA
TTATATAGAGAAACCTTTTACAGAAGGTAGATTAAAGTTAGTAGTTAAGAGAGCGATAGAATCTGGTAGATTGCGTAGAG
AAAATGACGAATTAAAATCAACATTTGAAGATTACGAAATAGTTGGCAACTCTCCTGTAATAAAAAATCTAAGGAGTATG
ATTAATAAAGCTGCTACAACGTCCAGTCGCATTTTAATCACTGGATCGCCTGGCGTAGGAAAAGAAGTAGTTGCTAGATT
AATACATAAAAAATCAAAAGGATATGATACACCGTTTATATCGATGTATTCATCTATGTTGCCTGCAAATAATTACTTAG
TTAATATATTTGGTAGTGAGGAAAGCAGTAACATTTTGTCTCATAGGGTACCACCTCATATTGGCATAATAGAACAAGCA
AATCATGGAACACTCTTTATAGATGAGGTTACAGATTTAAGATATGATACTCAGTTACGATTACTTAGGTTATTGCAAGA
AGGAAAAATATACAGAGAGAATAGCAAAATTCCTGTAAGTGTAGATGTGAGGATTATTGTTTCATCTTCAAAAGATATAG
AAAATGAAGTAAGAGCTGGGAAGTTTTGTGAGGATTTATATTATAGATTGAATGTTCTTCCAATTAGAGTACCGTCTCTA
GTAGAGTATTGTACTGATATTCCAGAGCTGTGTAGATATTTTATGAGTAGCATATGTAAGAAAATAGGATTGTGTACACA
TATATTGAGTGATGAAGCTTTAATAGCGATGCAATCTTATGAATGGCCAGGTAACTTACGTCAATTACGTAATGTTATAG
AGTGGATATTAATTATGAAATCTCCTAAAGAAATTATTACTGCAAAAGATTTGCCGGTAGATATAGTATCGAATTCTCCT
ATCAATGATGTTCTGAGTGCTAAAGTCATATCTGTTCCTCTTCGCCAAGCACGTGAGGAGTTTGAAAGACAGTATTTGAA
AACTCAGTTGTCACGTTTTGGAGGTAATGTGTCAAAAACTGCTGAATTTGTTGGTATGGAGCGTTCCGCACTTCATCGCA
AATTGAAAGTTTTAGGATTGTGTAGTATATCTGAGTAA

Upstream 100 bases:

>100_bases
TAATGTGTATAGTAGTATTATTAAGTGTACTTATATAGAGTACACTGTGTTTTGTGGTGTTTCATCAGTATTGTAGGTAA
TTATGGCACAGAATTTTGAA

Downstream 100 bases:

>100_bases
CTTATATTATGTGATGTTAGAATTAGTTTTTTAACTGTGTATGTGTTGTTTTAATTAAGACGATAAGTTATATATAACCA
AGAATACTGCATCTTATAGT

Product: putative nitrogen regulation protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDIWLKGSDIDGLSVLEKLKERY
PYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKLVVKRAIESGRLRRENDELKSTFEDYEIVGNSPVIKNLRSM
INKAATTSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESSNILSHRVPPHIGIIEQA
NHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSVDVRIIVSSSKDIENEVRAGKFCEDLYYRLNVLPIRVPSL
VEYCTDIPELCRYFMSSICKKIGLCTHILSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEIITAKDLPVDIVSNSP
INDVLSAKVISVPLRQAREEFERQYLKTQLSRFGGNVSKTAEFVGMERSALHRKLKVLGLCSISE

Sequences:

>Translated_465_residues
MSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDIWLKGSDIDGLSVLEKLKERY
PYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKLVVKRAIESGRLRRENDELKSTFEDYEIVGNSPVIKNLRSM
INKAATTSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESSNILSHRVPPHIGIIEQA
NHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSVDVRIIVSSSKDIENEVRAGKFCEDLYYRLNVLPIRVPSL
VEYCTDIPELCRYFMSSICKKIGLCTHILSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEIITAKDLPVDIVSNSP
INDVLSAKVISVPLRQAREEFERQYLKTQLSRFGGNVSKTAEFVGMERSALHRKLKVLGLCSISE
>Mature_464_residues
SKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDIWLKGSDIDGLSVLEKLKERYP
YLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKLVVKRAIESGRLRRENDELKSTFEDYEIVGNSPVIKNLRSMI
NKAATTSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESSNILSHRVPPHIGIIEQAN
HGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSVDVRIIVSSSKDIENEVRAGKFCEDLYYRLNVLPIRVPSLV
EYCTDIPELCRYFMSSICKKIGLCTHILSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEIITAKDLPVDIVSNSPI
NDVLSAKVISVPLRQAREEFERQYLKTQLSRFGGNVSKTAEFVGMERSALHRKLKVLGLCSISE

Specific function: Member of the two-component regulatory system RBE_0312/RBE_0470 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=33.1196581196581, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=31.7391304347826, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=31.7073170731707, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=28.6666666666667, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=31.8611987381703, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=32.1875, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=30.6122448979592, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789087, Length=318, Percent_Identity=32.7044025157233, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=31.6614420062696, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=28.2798833819242, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081872, Length=307, Percent_Identity=27.6872964169381, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=27.6527331189711, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=26.7241379310345, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787487, Length=145, Percent_Identity=28.2758620689655, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=34.1666666666667, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=29.4964028776978, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52644; Mature: 52513

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDI
CCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHCCCCCEEEEEE
WLKGSDIDGLSVLEKLKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKL
EEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHH
VVKRAIESGRLRRENDELKSTFEDYEIVGNSPVIKNLRSMINKAATTSSRILITGSPGVG
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
KEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESSNILSHRVPPHIGIIEQA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCCEEEEC
NHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSVDVRIIVSSSKDIENEVRAG
CCCEEEEECHHCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEEEEECCCCHHHHHHHH
KFCEDLYYRLNVLPIRVPSLVEYCTDIPELCRYFMSSICKKIGLCTHILSDEALIAMQSY
HHHHHHHHHHHEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
EWPGNLRQLRNVIEWILIMKSPKEIITAKDLPVDIVSNSPINDVLSAKVISVPLRQAREE
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
FERQYLKTQLSRFGGNVSKTAEFVGMERSALHRKLKVLGLCSISE
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
SKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDI
CCCCEEEEEEEEECCCCCHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHCCCCCEEEEEE
WLKGSDIDGLSVLEKLKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKL
EEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHH
VVKRAIESGRLRRENDELKSTFEDYEIVGNSPVIKNLRSMINKAATTSSRILITGSPGVG
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
KEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESSNILSHRVPPHIGIIEQA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCCEEEEC
NHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSVDVRIIVSSSKDIENEVRAG
CCCEEEEECHHCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEEEEECCCCHHHHHHHH
KFCEDLYYRLNVLPIRVPSLVEYCTDIPELCRYFMSSICKKIGLCTHILSDEALIAMQSY
HHHHHHHHHHHEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
EWPGNLRQLRNVIEWILIMKSPKEIITAKDLPVDIVSNSPINDVLSAKVISVPLRQAREE
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
FERQYLKTQLSRFGGNVSKTAEFVGMERSALHRKLKVLGLCSISE
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA