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The map label for this gene is ndk

Identifier: 88608733

GI number: 88608733

Start: 486392

End: 486811

Strand: Reverse

Name: ndk

Synonym: NSE_0560

Alternate gene names: 88608733

Gene position: 486811-486392 (Counterclockwise)

Preceding gene: 88608883

Following gene: 88608779

Centisome position: 56.67

GC content: 41.67

Gene sequence:

>420_bases
ATGAAGAGAACTTTATCTATTCTTAAGCCAGATGTTATTTCTAGGAATATAACAGGTAAGGTTAATGCCTATATCGAGGC
TGCCGGTCTGCAGATTATTGCGATGAAGCAACTTCATCTGACGCGTATTCAAGCCGAAGCTTTTTATGTGGTCCACAAAG
ATAGATTCTTTTTCAACGACCTGGTTAACTTTATGACATCTGCTCCCGTTATAGTGCAGGTATTATCAGGAGATGATGCT
GTTCATCGCTACAGAAAGCTTATGGGTGATACGGATCCAAAAAAGGCTGCAAAGGGAACGATCCGTGGTGACTTCGCCGA
GAGTATAGATGCAAACTGCGTTCATGGCTCTGACAGCGAAGAGAATGCTAAGAATGAAATTGCATTCTTTTTTAGTCGGT
GCGAGATTTTTGACAGATGA

Upstream 100 bases:

>100_bases
TCGCAAGTAGAGTATAGTAATGTACATAAGTGTTATGGATTTCTAAATCCGTTAAGTCTAAGCCCTTTGGTTGTTAAAAA
AAAGCATTTTAACATTAGTC

Downstream 100 bases:

>100_bases
TATCATGAGGGCACTATCGCTCTTCTGACTAAAAGTTCGAGCACAGTTTGTGTTTTTAGATCGCTTATGTATCTGAAACA
AAAAATTTTTAGGATACTTT

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MKRTLSILKPDVISRNITGKVNAYIEAAGLQIIAMKQLHLTRIQAEAFYVVHKDRFFFNDLVNFMTSAPVIVQVLSGDDA
VHRYRKLMGDTDPKKAAKGTIRGDFAESIDANCVHGSDSEENAKNEIAFFFSRCEIFDR

Sequences:

>Translated_139_residues
MKRTLSILKPDVISRNITGKVNAYIEAAGLQIIAMKQLHLTRIQAEAFYVVHKDRFFFNDLVNFMTSAPVIVQVLSGDDA
VHRYRKLMGDTDPKKAAKGTIRGDFAESIDANCVHGSDSEENAKNEIAFFFSRCEIFDR
>Mature_139_residues
MKRTLSILKPDVISRNITGKVNAYIEAAGLQIIAMKQLHLTRIQAEAFYVVHKDRFFFNDLVNFMTSAPVIVQVLSGDDA
VHRYRKLMGDTDPKKAAKGTIRGDFAESIDANCVHGSDSEENAKNEIAFFFSRCEIFDR

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family

Homologues:

Organism=Homo sapiens, GI66392203, Length=138, Percent_Identity=36.9565217391304, Blast_Score=106, Evalue=5e-24,
Organism=Homo sapiens, GI66392227, Length=138, Percent_Identity=36.9565217391304, Blast_Score=106, Evalue=5e-24,
Organism=Homo sapiens, GI66392205, Length=138, Percent_Identity=36.9565217391304, Blast_Score=106, Evalue=5e-24,
Organism=Homo sapiens, GI4505409, Length=138, Percent_Identity=36.9565217391304, Blast_Score=106, Evalue=5e-24,
Organism=Homo sapiens, GI66392192, Length=138, Percent_Identity=36.9565217391304, Blast_Score=106, Evalue=5e-24,
Organism=Homo sapiens, GI37693993, Length=136, Percent_Identity=36.7647058823529, Blast_Score=103, Evalue=6e-23,
Organism=Homo sapiens, GI4557797, Length=137, Percent_Identity=36.4963503649635, Blast_Score=102, Evalue=9e-23,
Organism=Homo sapiens, GI38045913, Length=137, Percent_Identity=36.4963503649635, Blast_Score=102, Evalue=9e-23,
Organism=Homo sapiens, GI4826862, Length=135, Percent_Identity=36.2962962962963, Blast_Score=97, Evalue=5e-21,
Organism=Homo sapiens, GI37574614, Length=138, Percent_Identity=36.231884057971, Blast_Score=88, Evalue=2e-18,
Organism=Homo sapiens, GI7019465, Length=138, Percent_Identity=36.231884057971, Blast_Score=88, Evalue=3e-18,
Organism=Homo sapiens, GI5031951, Length=135, Percent_Identity=35.5555555555556, Blast_Score=86, Evalue=1e-17,
Organism=Homo sapiens, GI30023828, Length=143, Percent_Identity=27.972027972028, Blast_Score=70, Evalue=5e-13,
Organism=Homo sapiens, GI148839372, Length=138, Percent_Identity=29.7101449275362, Blast_Score=67, Evalue=6e-12,
Organism=Homo sapiens, GI4505413, Length=135, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1788866, Length=139, Percent_Identity=48.2014388489209, Blast_Score=147, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17506807, Length=137, Percent_Identity=39.4160583941606, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322783, Length=137, Percent_Identity=35.036496350365, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45549037, Length=135, Percent_Identity=37.037037037037, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18860097, Length=136, Percent_Identity=33.0882352941176, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDK_NEOSM (Q2GDK4)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506442.1
- ProteinModelPortal:   Q2GDK4
- SMR:   Q2GDK4
- STRING:   Q2GDK4
- GeneID:   3932166
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0560
- TIGR:   NSE_0560
- eggNOG:   COG0105
- HOGENOM:   HBG445152
- OMA:   ERPFFGA
- PhylomeDB:   Q2GDK4
- ProtClustDB:   CLSK2528109
- BioCyc:   NSEN222891:NSE_0560-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00451
- InterPro:   IPR001564
- InterPro:   IPR023005
- Gene3D:   G3DSA:3.30.70.141
- PANTHER:   PTHR11349
- PRINTS:   PR01243
- SMART:   SM00562

Pfam domain/function: PF00334 NDK; SSF54919 NDK

EC number: =2.7.4.6

Molecular weight: Translated: 15671; Mature: 15671

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00469 NDP_KINASES

Important sites: ACT_SITE 115-115 BINDING 9-9 BINDING 57-57 BINDING 85-85 BINDING 91-91 BINDING 102-102 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTLSILKPDVISRNITGKVNAYIEAAGLQIIAMKQLHLTRIQAEAFYVVHKDRFFFND
CCCCHHHCCCHHHCCCCCCCEEEEEEECCEEEEEEHHHHHHHEEEEEEEEEECCCHHHHH
LVNFMTSAPVIVQVLSGDDAVHRYRKLMGDTDPKKAAKGTIRGDFAESIDANCVHGSDSE
HHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCCHHCCCCCCCCCCCCC
ENAKNEIAFFFSRCEIFDR
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRTLSILKPDVISRNITGKVNAYIEAAGLQIIAMKQLHLTRIQAEAFYVVHKDRFFFND
CCCCHHHCCCHHHCCCCCCCEEEEEEECCEEEEEEHHHHHHHEEEEEEEEEECCCHHHHH
LVNFMTSAPVIVQVLSGDDAVHRYRKLMGDTDPKKAAKGTIRGDFAESIDANCVHGSDSE
HHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCCHHCCCCCCCCCCCCC
ENAKNEIAFFFSRCEIFDR
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA