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The map label for this gene is ksgA

Identifier: 88608711

GI number: 88608711

Start: 295681

End: 296469

Strand: Reverse

Name: ksgA

Synonym: NSE_0362

Alternate gene names: 88608711

Gene position: 296469-295681 (Counterclockwise)

Preceding gene: 88608240

Following gene: 88608713

Centisome position: 34.51

GC content: 41.06

Gene sequence:

>789_bases
GTGCAACGATATAATAAACTACTTGGACAACACTTCATCTACGACAGGGAAGTGCTTGACAAAATAATCGACGCAGCAAC
ATCAGTAAAGGGCAAACACATCTTCGAAATAGGTGCTGGATCAGGAACATTGTCTGCCGCCATACTTCTGAGAGAACCAG
CTTCACTTATATCAGTCGAAAAAGACAAAAGATTTTCAGAATCGCTGTCTTCCCTCATGGCACAGTATCAAAATTACAAA
TACACCATAGGTGATGCTTTACTCATTCGACTAAGCAGTTTATTTAAACAAGAAAAGGTCACCATAATTGCAAATTTGCC
ATACAACATTGCTACGCATCTGTTGCTAGGATGGATGAACGAACTAGAGCAAGTTCGAGAAATGGTGCTAATGTTCCAAA
AAGAAGTAGCAGACCGTATTTGTGCACAGCCTAAAAGCAAAAACTATGGCGCTTTGTCTGTTCTAGTGCAACTAGAATGC
AAAGCGGAGTCACAATTTGCCCTCGCACCAGAAGTTTTCACACCACCACCAAGAGTAACATCTACAGTCCTGAAGCTTAC
ACCACTGAAAAACAAATGGCCAAGAAACAAACCTGTTTTGGAAAAGATCCTGACAGAAGGTTTTTCCCAGCGAAGAAAAA
TGATCAAAAAAAGCCTATCAAGAATTTTCAAAGATTCTGAAGCACTACATTCCGCATTAGCGCAGGTAGGAGCCAGTCCT
ACAATGAGAATAGAGGAACTTAACCCCGAGCAACTCTGCAGGCTTTCTTGTATAGCAGAAATGGACTAA

Upstream 100 bases:

>100_bases
AAAAATAAATGGATAAGCAAACAGAAATCGGGCTTTGCTTCAGACGAAGTACTACGGCTATTATTTGAGTGACGAACTCA
TTTATCAGAACAAATAAAAG

Downstream 100 bases:

>100_bases
CTTCGAGAGAGCAACGCGTCTTTAAGCTTCGCACGAGAAAGTTAACTTCAATAAGTTGATTCCTCCTTGTAAAACACCCA
AGACGCACAAACGATAAAAC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQRYNKLLGQHFIYDREVLDKIIDAATSVKGKHIFEIGAGSGTLSAAILLREPASLISVEKDKRFSESLSSLMAQYQNYK
YTIGDALLIRLSSLFKQEKVTIIANLPYNIATHLLLGWMNELEQVREMVLMFQKEVADRICAQPKSKNYGALSVLVQLEC
KAESQFALAPEVFTPPPRVTSTVLKLTPLKNKWPRNKPVLEKILTEGFSQRRKMIKKSLSRIFKDSEALHSALAQVGASP
TMRIEELNPEQLCRLSCIAEMD

Sequences:

>Translated_262_residues
MQRYNKLLGQHFIYDREVLDKIIDAATSVKGKHIFEIGAGSGTLSAAILLREPASLISVEKDKRFSESLSSLMAQYQNYK
YTIGDALLIRLSSLFKQEKVTIIANLPYNIATHLLLGWMNELEQVREMVLMFQKEVADRICAQPKSKNYGALSVLVQLEC
KAESQFALAPEVFTPPPRVTSTVLKLTPLKNKWPRNKPVLEKILTEGFSQRRKMIKKSLSRIFKDSEALHSALAQVGASP
TMRIEELNPEQLCRLSCIAEMD
>Mature_262_residues
MQRYNKLLGQHFIYDREVLDKIIDAATSVKGKHIFEIGAGSGTLSAAILLREPASLISVEKDKRFSESLSSLMAQYQNYK
YTIGDALLIRLSSLFKQEKVTIIANLPYNIATHLLLGWMNELEQVREMVLMFQKEVADRICAQPKSKNYGALSVLVQLEC
KAESQFALAPEVFTPPPRVTSTVLKLTPLKNKWPRNKPVLEKILTEGFSQRRKMIKKSLSRIFKDSEALHSALAQVGASP
TMRIEELNPEQLCRLSCIAEMD

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=266, Percent_Identity=30.4511278195489, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI7657198, Length=226, Percent_Identity=27.8761061946903, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=33.852140077821, Blast_Score=137, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25146882, Length=228, Percent_Identity=29.3859649122807, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=283, Percent_Identity=26.5017667844523, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=196, Percent_Identity=30.1020408163265, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357273, Length=273, Percent_Identity=31.8681318681319, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21358017, Length=194, Percent_Identity=31.4432989690722, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NEOSM (Q2GE45)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506251.1
- STRING:   Q2GE45
- GeneID:   3931545
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0362
- TIGR:   NSE_0362
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q2GE45
- ProtClustDB:   PRK00274
- BioCyc:   NSEN222891:NSE_0362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29599; Mature: 29599

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 11-11 BINDING 13-13 BINDING 38-38 BINDING 60-60 BINDING 85-85 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYNKLLGQHFIYDREVLDKIIDAATSVKGKHIFEIGAGSGTLSAAILLREPASLISVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHH
KDKRFSESLSSLMAQYQNYKYTIGDALLIRLSSLFKQEKVTIIANLPYNIATHLLLGWMN
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHH
ELEQVREMVLMFQKEVADRICAQPKSKNYGALSVLVQLECKAESQFALAPEVFTPPPRVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCHHH
STVLKLTPLKNKWPRNKPVLEKILTEGFSQRRKMIKKSLSRIFKDSEALHSALAQVGASP
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TMRIEELNPEQLCRLSCIAEMD
CEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRYNKLLGQHFIYDREVLDKIIDAATSVKGKHIFEIGAGSGTLSAAILLREPASLISVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHH
KDKRFSESLSSLMAQYQNYKYTIGDALLIRLSSLFKQEKVTIIANLPYNIATHLLLGWMN
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHH
ELEQVREMVLMFQKEVADRICAQPKSKNYGALSVLVQLECKAESQFALAPEVFTPPPRVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCHHH
STVLKLTPLKNKWPRNKPVLEKILTEGFSQRRKMIKKSLSRIFKDSEALHSALAQVGASP
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TMRIEELNPEQLCRLSCIAEMD
CEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA