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The map label for this gene is ribE-1

Identifier: 88608667

GI number: 88608667

Start: 325597

End: 326046

Strand: Reverse

Name: ribE-1

Synonym: NSE_0401

Alternate gene names: 88608667

Gene position: 326046-325597 (Counterclockwise)

Preceding gene: 88608477

Following gene: 88608339

Centisome position: 37.96

GC content: 40.67

Gene sequence:

>450_bases
ATGCAAAAAAAGGTCCTAGTAATTTGCAGTAAGGTCAACCCTAATATCTGCAAGGTGTTATATAAAACTGCAGCAAAACA
TCTTAAACAACAGGGGGTAGCTTGCAAGTCAGTTTATGTACCGGGCGCATTTGAGTTGCCTACCGCACTTAACATTTTAC
ATTCTAAAGATTTTGCTGGTTACGTCATCTTAGGATGCATCGTTAAATCTGGTACCCCACATTTCGATTATGTTTCGTCC
GCGGCCTGTCGTGGGATTATGGATGTCGCCATCAAGAATAACCTTGCCATAGGCTTCGGCGTTATAACAGCAAATAACAT
GGAGCAAGCAAATGAGAGATCTGTTTCTTATGGAATTGCAGCTGTAAATGCCTGCATGGCAATGATCAAGCTAAAAGAAA
GAAGTCCAACACTGTCAGAAGAAGATTTCTTTGAAGATACAGATCTGTAA

Upstream 100 bases:

>100_bases
TTTTTGATGTTTATTCCTCCACTTTCTATTGGAAGTTACTTCACTTTTTTTTACAGCCGACCGATGCTAGAATCACTCGG
TTTTCTGTATTAATTAAGCA

Downstream 100 bases:

>100_bases
GTACTCTTTCCTTCTCGGAAAGTTAATTTGTTCTTTAACTATGTTTGCTGGGATATTCTGCTAAATTGGAGCAGAACAGT
AATTGTAGAGCTTTTACCAA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MQKKVLVICSKVNPNICKVLYKTAAKHLKQQGVACKSVYVPGAFELPTALNILHSKDFAGYVILGCIVKSGTPHFDYVSS
AACRGIMDVAIKNNLAIGFGVITANNMEQANERSVSYGIAAVNACMAMIKLKERSPTLSEEDFFEDTDL

Sequences:

>Translated_149_residues
MQKKVLVICSKVNPNICKVLYKTAAKHLKQQGVACKSVYVPGAFELPTALNILHSKDFAGYVILGCIVKSGTPHFDYVSS
AACRGIMDVAIKNNLAIGFGVITANNMEQANERSVSYGIAAVNACMAMIKLKERSPTLSEEDFFEDTDL
>Mature_149_residues
MQKKVLVICSKVNPNICKVLYKTAAKHLKQQGVACKSVYVPGAFELPTALNILHSKDFAGYVILGCIVKSGTPHFDYVSS
AACRGIMDVAIKNNLAIGFGVITANNMEQANERSVSYGIAAVNACMAMIKLKERSPTLSEEDFFEDTDL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=116, Percent_Identity=33.6206896551724, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 16163; Mature: 16163

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKVLVICSKVNPNICKVLYKTAAKHLKQQGVACKSVYVPGAFELPTALNILHSKDFAG
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCCC
YVILGCIVKSGTPHFDYVSSAACRGIMDVAIKNNLAIGFGVITANNMEQANERSVSYGIA
HHHEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHH
AVNACMAMIKLKERSPTLSEEDFFEDTDL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKKVLVICSKVNPNICKVLYKTAAKHLKQQGVACKSVYVPGAFELPTALNILHSKDFAG
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCCC
YVILGCIVKSGTPHFDYVSSAACRGIMDVAIKNNLAIGFGVITANNMEQANERSVSYGIA
HHHEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHH
AVNACMAMIKLKERSPTLSEEDFFEDTDL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA