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The map label for this gene is purN

Identifier: 88608663

GI number: 88608663

Start: 391656

End: 392234

Strand: Reverse

Name: purN

Synonym: NSE_0474

Alternate gene names: 88608663

Gene position: 392234-391656 (Counterclockwise)

Preceding gene: 88608537

Following gene: 88608561

Centisome position: 45.66

GC content: 42.49

Gene sequence:

>579_bases
ATGAGAAAAAAGGTTGCCATTTTTATTTCAGGCAGGGGCTCGAACATGAAGTCCCTGCTGGAGTTTTCCAAAAATGAAGG
AAAAAAAATTTTCTCTGTTGCTCTTGTTATTTCAAATAAGCCCGATGCTGCTGGTATTAGCATAGCCCACACTTACGGCA
TAGATACACGAATTTGCACCTCAGAGGAGGAAATACTCACTGTACTGTCATATGTAAAGGTGGATCTTATATGTTTGGCC
GGTTTCATGAAAATACTAAGTAAGGACTTCATATCGAGAGTTGGGTGTGACATCATCAATATTCACCCTTCCTTACTACC
ATCATTCAGGGGATTAAACGCCCAAGCAGAAGCGCTGGCAGCAGGGGTAAAGATCGCTGGGTGCACTGTGCACTACGTCA
CCCCCGAGGTTGATGCAGGAAAAATAATTGTCCAAGGTGCCGTTCCGGTGCTTAAAAACGACACTGTCAAATCACTAAGT
GAGAGAATACTCAAAGCTGAGCACAAATGTTTCCCAATTGCCGTTGAAAAGGTACTTACCGACAATGTAGAGGAAGACTT
TATTATTTTACAGGGATAA

Upstream 100 bases:

>100_bases
CGGAATTTATATCGGAATCAGTTAAAGAAACTTACAAGTAAATCTTTGCTTTGCTTACGTACCTATAGCAGGAAATAAGA
CTCTGCGTTTTGAAGGTTGA

Downstream 100 bases:

>100_bases
AACGCAAATGAAGAAACAATAAATAAGACTGCAACTTTTCTACATAAAAAATACATACTCAGGTTTTCATACCCATTGAA
GAAAGGTAGTTAGTTCGTAT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MRKKVAIFISGRGSNMKSLLEFSKNEGKKIFSVALVISNKPDAAGISIAHTYGIDTRICTSEEEILTVLSYVKVDLICLA
GFMKILSKDFISRVGCDIINIHPSLLPSFRGLNAQAEALAAGVKIAGCTVHYVTPEVDAGKIIVQGAVPVLKNDTVKSLS
ERILKAEHKCFPIAVEKVLTDNVEEDFIILQG

Sequences:

>Translated_192_residues
MRKKVAIFISGRGSNMKSLLEFSKNEGKKIFSVALVISNKPDAAGISIAHTYGIDTRICTSEEEILTVLSYVKVDLICLA
GFMKILSKDFISRVGCDIINIHPSLLPSFRGLNAQAEALAAGVKIAGCTVHYVTPEVDAGKIIVQGAVPVLKNDTVKSLS
ERILKAEHKCFPIAVEKVLTDNVEEDFIILQG
>Mature_192_residues
MRKKVAIFISGRGSNMKSLLEFSKNEGKKIFSVALVISNKPDAAGISIAHTYGIDTRICTSEEEILTVLSYVKVDLICLA
GFMKILSKDFISRVGCDIINIHPSLLPSFRGLNAQAEALAAGVKIAGCTVHYVTPEVDAGKIIVQGAVPVLKNDTVKSLS
ERILKAEHKCFPIAVEKVLTDNVEEDFIILQG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=192, Percent_Identity=45.3125, Blast_Score=165, Evalue=3e-41,
Organism=Homo sapiens, GI209869995, Length=192, Percent_Identity=45.3125, Blast_Score=165, Evalue=3e-41,
Organism=Homo sapiens, GI209869993, Length=192, Percent_Identity=45.3125, Blast_Score=165, Evalue=3e-41,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=34.8066298342541, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787483, Length=190, Percent_Identity=27.8947368421053, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=45.3038674033149, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=210, Percent_Identity=28.0952380952381, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=45.1086956521739, Blast_Score=156, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20802; Mature: 20802

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKVAIFISGRGSNMKSLLEFSKNEGKKIFSVALVISNKPDAAGISIAHTYGIDTRICT
CCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
SEEEILTVLSYVKVDLICLAGFMKILSKDFISRVGCDIINIHPSLLPSFRGLNAQAEALA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCCCHHHHHHH
AGVKIAGCTVHYVTPEVDAGKIIVQGAVPVLKNDTVKSLSERILKAEHKCFPIAVEKVLT
CCEEEECEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
DNVEEDFIILQG
CCCCCCEEEEEC
>Mature Secondary Structure
MRKKVAIFISGRGSNMKSLLEFSKNEGKKIFSVALVISNKPDAAGISIAHTYGIDTRICT
CCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
SEEEILTVLSYVKVDLICLAGFMKILSKDFISRVGCDIINIHPSLLPSFRGLNAQAEALA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCCCHHHHHHH
AGVKIAGCTVHYVTPEVDAGKIIVQGAVPVLKNDTVKSLSERILKAEHKCFPIAVEKVLT
CCEEEECEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
DNVEEDFIILQG
CCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]