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The map label for this gene is pepA

Identifier: 88608537

GI number: 88608537

Start: 392294

End: 393814

Strand: Reverse

Name: pepA

Synonym: NSE_0475

Alternate gene names: 88608537

Gene position: 393814-392294 (Counterclockwise)

Preceding gene: 88608103

Following gene: 88608663

Centisome position: 45.85

GC content: 41.75

Gene sequence:

>1521_bases
ATGGAAGTCTTTTTTTTGAATCCAGTAGAGGCAATGCAAAGCGAGCCTATTCTATTCAGTGGACTGTATGAAAATTCTGA
GTTCTTTGGAAGGATTTCCATTTTGGATCAACAAAGCTCTTCGTTTATCTCCAAAAGCATTGCTTCGACTTCGTTTACTG
GAAAAGTTGGGGAAAACGTTTGTATTTTTCTCAGTGACCTTGTCGAAGGGTATCCAAAACTGCAACAACTGTGTCTCATT
GGGCTTGGAAAACAAAAAGATTTTGATGCTCATACCGCAGAGAAAACAGGAGCACAGATTGCTTCACTCATGAAGAAGAA
TAATGTTTCTTCCGCGACAGTACTTTTAGATGGTTTAAAAGATGACTATGCCTTGGATCTGGCTTTTGGGGCAAAGCTAA
AAGACTATTCGTTCGATAAATATAAAACAAAAAAGACCGATGATAAGAGCATAACGCTAGAGCGGCTTGTAATTGGAATT
GAGAACTACGAGCACATCTATTCGGTTTTTCGAGAGAAAGTCGAGCCTTTAATTGAATCAATGAAGTTAACTAGGGATCT
GATAAATGAACCCGCAAATCACCTTAATCCGGAAACTTATGCCAATGCTGTAAAGGAGATTACCAAGACAGCTCCAAACC
TAAAAGTCGAAATCCTTGATGAAAAGGGCATGCAGAAATTAGGCATGAACGCACTTCTAGGCGTTGGTCAGGGAAGCACT
TACCCTTCAAAATTAGTGGTGGTAAAATATAACGGTGCAGTGGATAAAGATGATCCCTACATCGCTCTTGTTGGAAAAGG
CGTTACTTTCGACAGCGGTGGTATTTCACTTAAGCCAGCACGTGGAATGTGGCATATGGTATCCGATATGGCTGGCTCTG
CAACAGTTTTGGGGGCAGTTCATGCTATTGCAAAACAGGGTGTGAAAGCAAATGTTGCAGCCGTACTCGGGATCGTTGAA
AACGCAGTTTCAGGAGTCGCACAACGTCCAGGGGACATAGTCAGGTCGGCTTCTGGGAAAACAATTGAAGTACTCAACAC
TGACGCAGAAGGTAGGTTGGTACTCGCAGATGCCTTGTGGTATGCGCAAGAACACTTAAAAGCAAACCAAGTAATAGACG
TAGCAACACTCACAGGAGCAATAGTTGTTGCTTTAGGACACGATCATGCAGGATTGTTCTCTAATGATGATGCATTAGCC
GAAAACCTTGCAAATGTGGGGAAAAAGGTTGGTGAGAAACTCTGGAGAATGCCTATGTCAAAAAATTATGATGATCTCAT
AAATTCTGAAGTCGCAGATGTGAAGAACATCTCTACAGAAAATCATGGCGCAGACAGCATAACAGCAGCTCAATTCTTGA
AACGATTCATAAACGACGGAACAAAATGGGCTCACCTCGATATTGCCGGCGTTGCATGGAATAACAGTACTTCCCATTTT
TCCAGCATTGGAGCTTCTGGATTTGGTGTACGACTACTAACGGAATTTATATCGGAATCAGTTAAAGAAACTTACAAGTA
A

Upstream 100 bases:

>100_bases
TAGAGCGCCAGTTTGTGGTACTGGATGTCGCCAGTTCGAATCTGGTCGTTCGCCCGTTGTTTTTTCGAAGCACCACTCTG
TTCTTTTTCTAGGAGTTATT

Downstream 100 bases:

>100_bases
ATCTTTGCTTTGCTTACGTACCTATAGCAGGAAATAAGACTCTGCGTTTTGAAGGTTGAATGAGAAAAAAGGTTGCCATT
TTTATTTCAGGCAGGGGCTC

Product: cytosol aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MEVFFLNPVEAMQSEPILFSGLYENSEFFGRISILDQQSSSFISKSIASTSFTGKVGENVCIFLSDLVEGYPKLQQLCLI
GLGKQKDFDAHTAEKTGAQIASLMKKNNVSSATVLLDGLKDDYALDLAFGAKLKDYSFDKYKTKKTDDKSITLERLVIGI
ENYEHIYSVFREKVEPLIESMKLTRDLINEPANHLNPETYANAVKEITKTAPNLKVEILDEKGMQKLGMNALLGVGQGST
YPSKLVVVKYNGAVDKDDPYIALVGKGVTFDSGGISLKPARGMWHMVSDMAGSATVLGAVHAIAKQGVKANVAAVLGIVE
NAVSGVAQRPGDIVRSASGKTIEVLNTDAEGRLVLADALWYAQEHLKANQVIDVATLTGAIVVALGHDHAGLFSNDDALA
ENLANVGKKVGEKLWRMPMSKNYDDLINSEVADVKNISTENHGADSITAAQFLKRFINDGTKWAHLDIAGVAWNNSTSHF
SSIGASGFGVRLLTEFISESVKETYK

Sequences:

>Translated_506_residues
MEVFFLNPVEAMQSEPILFSGLYENSEFFGRISILDQQSSSFISKSIASTSFTGKVGENVCIFLSDLVEGYPKLQQLCLI
GLGKQKDFDAHTAEKTGAQIASLMKKNNVSSATVLLDGLKDDYALDLAFGAKLKDYSFDKYKTKKTDDKSITLERLVIGI
ENYEHIYSVFREKVEPLIESMKLTRDLINEPANHLNPETYANAVKEITKTAPNLKVEILDEKGMQKLGMNALLGVGQGST
YPSKLVVVKYNGAVDKDDPYIALVGKGVTFDSGGISLKPARGMWHMVSDMAGSATVLGAVHAIAKQGVKANVAAVLGIVE
NAVSGVAQRPGDIVRSASGKTIEVLNTDAEGRLVLADALWYAQEHLKANQVIDVATLTGAIVVALGHDHAGLFSNDDALA
ENLANVGKKVGEKLWRMPMSKNYDDLINSEVADVKNISTENHGADSITAAQFLKRFINDGTKWAHLDIAGVAWNNSTSHF
SSIGASGFGVRLLTEFISESVKETYK
>Mature_506_residues
MEVFFLNPVEAMQSEPILFSGLYENSEFFGRISILDQQSSSFISKSIASTSFTGKVGENVCIFLSDLVEGYPKLQQLCLI
GLGKQKDFDAHTAEKTGAQIASLMKKNNVSSATVLLDGLKDDYALDLAFGAKLKDYSFDKYKTKKTDDKSITLERLVIGI
ENYEHIYSVFREKVEPLIESMKLTRDLINEPANHLNPETYANAVKEITKTAPNLKVEILDEKGMQKLGMNALLGVGQGST
YPSKLVVVKYNGAVDKDDPYIALVGKGVTFDSGGISLKPARGMWHMVSDMAGSATVLGAVHAIAKQGVKANVAAVLGIVE
NAVSGVAQRPGDIVRSASGKTIEVLNTDAEGRLVLADALWYAQEHLKANQVIDVATLTGAIVVALGHDHAGLFSNDDALA
ENLANVGKKVGEKLWRMPMSKNYDDLINSEVADVKNISTENHGADSITAAQFLKRFINDGTKWAHLDIAGVAWNNSTSHF
SSIGASGFGVRLLTEFISESVKETYK

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=428, Percent_Identity=34.8130841121495, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI47155554, Length=375, Percent_Identity=32.2666666666667, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1790710, Length=496, Percent_Identity=35.4838709677419, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI87082123, Length=332, Percent_Identity=37.6506024096386, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17556903, Length=302, Percent_Identity=38.7417218543046, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17565172, Length=360, Percent_Identity=29.7222222222222, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24661038, Length=325, Percent_Identity=33.8461538461538, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI21355725, Length=322, Percent_Identity=33.8509316770186, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI20129969, Length=396, Percent_Identity=29.7979797979798, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI161077148, Length=441, Percent_Identity=28.3446712018141, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI20130057, Length=441, Percent_Identity=28.3446712018141, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24662227, Length=397, Percent_Identity=28.7153652392947, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21357381, Length=318, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221379063, Length=320, Percent_Identity=33.125, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221379062, Length=320, Percent_Identity=33.125, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21355645, Length=499, Percent_Identity=27.4549098196393, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24662223, Length=499, Percent_Identity=27.4549098196393, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI19922386, Length=361, Percent_Identity=31.8559556786704, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI20129963, Length=326, Percent_Identity=32.8220858895706, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24646701, Length=251, Percent_Identity=27.0916334661355, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646703, Length=251, Percent_Identity=27.0916334661355, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358201, Length=251, Percent_Identity=27.0916334661355, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283 [H]

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 54807; Mature: 54807

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFFLNPVEAMQSEPILFSGLYENSEFFGRISILDQQSSSFISKSIASTSFTGKVGENV
CEEEEECCHHHHCCCCEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCC
CIFLSDLVEGYPKLQQLCLIGLGKQKDFDAHTAEKTGAQIASLMKKNNVSSATVLLDGLK
EEEHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
DDYALDLAFGAKLKDYSFDKYKTKKTDDKSITLERLVIGIENYEHIYSVFREKVEPLIES
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MKLTRDLINEPANHLNPETYANAVKEITKTAPNLKVEILDEKGMQKLGMNALLGVGQGST
HHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCHHHEECCCCCC
YPSKLVVVKYNGAVDKDDPYIALVGKGVTFDSGGISLKPARGMWHMVSDMAGSATVLGAV
CCCEEEEEEECCCCCCCCCEEEEEECCEEECCCCEEECCCCCHHHHHHHCCCCHHHHHHH
HAIAKQGVKANVAAVLGIVENAVSGVAQRPGDIVRSASGKTIEVLNTDAEGRLVLADALW
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCCEEEHHHHH
YAQEHLKANQVIDVATLTGAIVVALGHDHAGLFSNDDALAENLANVGKKVGEKLWRMPMS
HHHHHHCCCCEEEHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
KNYDDLINSEVADVKNISTENHGADSITAAQFLKRFINDGTKWAHLDIAGVAWNNSTSHF
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHH
SSIGASGFGVRLLTEFISESVKETYK
HHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEVFFLNPVEAMQSEPILFSGLYENSEFFGRISILDQQSSSFISKSIASTSFTGKVGENV
CEEEEECCHHHHCCCCEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCC
CIFLSDLVEGYPKLQQLCLIGLGKQKDFDAHTAEKTGAQIASLMKKNNVSSATVLLDGLK
EEEHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
DDYALDLAFGAKLKDYSFDKYKTKKTDDKSITLERLVIGIENYEHIYSVFREKVEPLIES
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MKLTRDLINEPANHLNPETYANAVKEITKTAPNLKVEILDEKGMQKLGMNALLGVGQGST
HHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCHHHEECCCCCC
YPSKLVVVKYNGAVDKDDPYIALVGKGVTFDSGGISLKPARGMWHMVSDMAGSATVLGAV
CCCEEEEEEECCCCCCCCCEEEEEECCEEECCCCEEECCCCCHHHHHHHCCCCHHHHHHH
HAIAKQGVKANVAAVLGIVENAVSGVAQRPGDIVRSASGKTIEVLNTDAEGRLVLADALW
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCCEEEHHHHH
YAQEHLKANQVIDVATLTGAIVVALGHDHAGLFSNDDALAENLANVGKKVGEKLWRMPMS
HHHHHHCCCCEEEHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
KNYDDLINSEVADVKNISTENHGADSITAAQFLKRFINDGTKWAHLDIAGVAWNNSTSHF
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHH
SSIGASGFGVRLLTEFISESVKETYK
HHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA