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The map label for this gene is rpoH

Identifier: 88608622

GI number: 88608622

Start: 279854

End: 280720

Strand: Direct

Name: rpoH

Synonym: NSE_0341

Alternate gene names: 88608622

Gene position: 279854-280720 (Clockwise)

Preceding gene: 88608693

Following gene: 88608360

Centisome position: 32.58

GC content: 43.02

Gene sequence:

>867_bases
ATGAGAGAGCTCTTATTACCCGTTGATAATTCTGAGTTTGATGCCTATGCACGGAAGATCTTAGCAATTCCGATACTGCA
GGAGGAAGAAGAACGCGAGCTTATAGATAGGTGGTTTGTTCACAGGGATATTTCGGCGGCTCAGAGGCTTGTTGAGGCCT
ATTTGAGGCTGGTAGTTAAGGTGACTGTACGATTTAGAAATTATGGCTTTCCTCTTATGGATCTTGCTTCCGAGGGAAAC
ATTGGTCTGATGAAGGCTATAAAAAATTTTAACCCGACACTCGGGCATGGAATCGCTACTTATGCAATATGGTGGATAAA
AGCTGCTATAAATGAGTATGTCATAAGGTCTTGGTCCATGGTGAAAATTGGTACGACTGCTGCTCAGCGGAGACTCTTCT
TTAATTTGCGCAAGTTGAAGAATTCTGGTAGTGTTGCTGGTCTGGATGAGAACCAGGTGGTGAAAAGGATTTCAGAAATT
CTTGCAGTATCAAAAAATGATGTAGAGGAAATGGAAAGGATTCTAAATGCTCGGGATGTTTCTCTGAATATGCAGTTGGG
CGAGGATGATAGTTCGGAGGTTCACGATCTCGTTCCTTTTCCAGAGGCAAATCAGGAGGCTGTTCTTTTAGAAACACAGG
AGAGACAGATGAAAAGTCAGTTGCTTTATGAGGCAGTTGAGGCACTTGAAGCGCGCCATAAAGAGATTTTTGTTGCCAGG
AGCCTAAGTGCAGTTCCTAGGACGCTTAAAGAGCTTGGGGAGAAATTTTCTATTTCTCAAGAGAGGGTTAGGCAGATACA
TGCTCGGGCAATGAAGAAAGTGACAGAGTATGTGGAAAAGCATGGAAGAAAACTTGGGATAGTATAA

Upstream 100 bases:

>100_bases
ACGTACTTCCCTTTTTGTCAGCGGATAGAAAAATCATTATAAGCAGAATGTAATCATGAGGTAGGTATGCTATAATCTCT
TAGTTTTTACTGTTGTTTGC

Downstream 100 bases:

>100_bases
CCGAACATGACCTTGCGTTTATCTGTACCGTTGGAACAGAATCAGCGTCAGAGTTTGCTTTGTCCGAAAATAGCGGTTAT
CGGAGTCGGCGGGGCAGGTG

Product: RNA polymerase sigma-32 factor

Products: diphosphate; RNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRELLLPVDNSEFDAYARKILAIPILQEEEERELIDRWFVHRDISAAQRLVEAYLRLVVKVTVRFRNYGFPLMDLASEGN
IGLMKAIKNFNPTLGHGIATYAIWWIKAAINEYVIRSWSMVKIGTTAAQRRLFFNLRKLKNSGSVAGLDENQVVKRISEI
LAVSKNDVEEMERILNARDVSLNMQLGEDDSSEVHDLVPFPEANQEAVLLETQERQMKSQLLYEAVEALEARHKEIFVAR
SLSAVPRTLKELGEKFSISQERVRQIHARAMKKVTEYVEKHGRKLGIV

Sequences:

>Translated_288_residues
MRELLLPVDNSEFDAYARKILAIPILQEEEERELIDRWFVHRDISAAQRLVEAYLRLVVKVTVRFRNYGFPLMDLASEGN
IGLMKAIKNFNPTLGHGIATYAIWWIKAAINEYVIRSWSMVKIGTTAAQRRLFFNLRKLKNSGSVAGLDENQVVKRISEI
LAVSKNDVEEMERILNARDVSLNMQLGEDDSSEVHDLVPFPEANQEAVLLETQERQMKSQLLYEAVEALEARHKEIFVAR
SLSAVPRTLKELGEKFSISQERVRQIHARAMKKVTEYVEKHGRKLGIV
>Mature_288_residues
MRELLLPVDNSEFDAYARKILAIPILQEEEERELIDRWFVHRDISAAQRLVEAYLRLVVKVTVRFRNYGFPLMDLASEGN
IGLMKAIKNFNPTLGHGIATYAIWWIKAAINEYVIRSWSMVKIGTTAAQRRLFFNLRKLKNSGSVAGLDENQVVKRISEI
LAVSKNDVEEMERILNARDVSLNMQLGEDDSSEVHDLVPFPEANQEAVLLETQERQMKSQLLYEAVEALEARHKEIFVAR
SLSAVPRTLKELGEKFSISQERVRQIHARAMKKVTEYVEKHGRKLGIV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=38.3512544802867, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=30.03663003663, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=26.4, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: 2.7.7.6

Molecular weight: Translated: 33089; Mature: 33089

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00716 SIGMA70_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELLLPVDNSEFDAYARKILAIPILQEEEERELIDRWFVHRDISAAQRLVEAYLRLVVK
CCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVRFRNYGFPLMDLASEGNIGLMKAIKNFNPTLGHGIATYAIWWIKAAINEYVIRSWSM
HHHHHHCCCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VKIGTTAAQRRLFFNLRKLKNSGSVAGLDENQVVKRISEILAVSKNDVEEMERILNARDV
EEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCHHCC
SLNMQLGEDDSSEVHDLVPFPEANQEAVLLETQERQMKSQLLYEAVEALEARHKEIFVAR
EEEEEECCCCCHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSAVPRTLKELGEKFSISQERVRQIHARAMKKVTEYVEKHGRKLGIV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MRELLLPVDNSEFDAYARKILAIPILQEEEERELIDRWFVHRDISAAQRLVEAYLRLVVK
CCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVRFRNYGFPLMDLASEGNIGLMKAIKNFNPTLGHGIATYAIWWIKAAINEYVIRSWSM
HHHHHHCCCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VKIGTTAAQRRLFFNLRKLKNSGSVAGLDENQVVKRISEILAVSKNDVEEMERILNARDV
EEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCHHCC
SLNMQLGEDDSSEVHDLVPFPEANQEAVLLETQERQMKSQLLYEAVEALEARHKEIFVAR
EEEEEECCCCCHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSAVPRTLKELGEKFSISQERVRQIHARAMKKVTEYVEKHGRKLGIV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]